Objective To investigate the dietary nutrition status of plateau frontier forces and to make recommendations on food and nutrients intake for plateau forces.Methods The dietary status of the plateau forces located at an altitude of 4600 m and 4030 m was investigated by weighing method.The dietary ration for soldiers, their daily dietary allowance and dietary balance index were used to evaluate their dietary patterns and nutrient intake.The concentrations of serum vitamin A and 25-OH vitamin D(25-OH VD) were detected by high performance liquid chromatography (HPLC).The arm muscle circumference and body fat rate of soldiers were evaluated by GJB 1636A-2016 and body composition related standard respectively.Results The intake of eggs, milk and plant oil of Unit A was sufficient, while the rest of the food intake did not reach the military standard;The intake of grain and plant oil of Unit B was sufficient,but the rest of the food intake was insufficient.The intake of protein,calcium,phosphonium,sodium,iron,selenium,iodine,copper,manganese,vitamin E and vitamin B3 of Unit A were adequate, but that of energy,potassium,zinc,magnesium,vitamin D,vitamin C,vitamin B1 and vitamin B2 was insufficient, and the intake of vitamin A,vitamin B6,vitamin B9 and vitamin B12 of Unit A was deficient.The intake of energy, protein,vitamin E,vitamin C,vitamin B1 and vitamin B3,and most of the minerals was adequate in Unit B, but vitamin B2 was insufficient.Furthermore, the intake of calcium,iodine,vitamin A,vitamin D and vitamin B was deficient.The proportion of nutrients which supply energy and the energy distribution of three meals in the two units were imperfect.The concentration of plasma vitamin A in both units was sufficient,but the concentration of plasma 25-OH VD was deficient.93.5% soldiers of unit A and 97.7% soldiers of unit B reached the standard of proper muscle circumference,80.6% soldiers of unit A and 70.5% soldiers of unit B had a low body fat rate.Conclusion The dietary structure of plateau border forces is not balanced.The intake of some foods and nutrients is insufficient so that nutritional education is badly needed to improve the dietary status of plateau forces.

Objective To investigate the levels of nutritional knowledge, attitude and dietary habits and its influential factors of the officers and soldiers in frontier forces to provide the theory basis for nutrition education.Methods Using self-designed nutritional knowledge-altitude-practice (KAP) questionnaires to survey 90 officers and soldiers who lived in plateau for at least 6 months. Questionnaires were handed out and handed over on the spot. According toMilitary Nutrition Survey and Evaluation Methods (GJB 1636A-2016), we measured the physical index of officers and soldiers, including height, weight and skin thickness of triceps. And we also evaluated body mass index (BMI) and upper arm muscle circumference.Results Nutrition knowledge, attitude and practice conditions of plateau forces were at the average, better and average levels respectively. Nutritional knowledge was significantly positively related to the practices (r=0.283,P=0.003), and was also significantly positively related to the age of the soldiers (r=0.228,P=0.04). Nutritional attitude was positively related to source of officers and soldiers (r=0.339,P=0.035), and nutritional practices were positively related to the upper arm muscle circumference (r=0.222,P=0.030). 65.6% of the officers and soldiers would like to know the knowledge about relationship between diet and disease. 25.6% of the officers and soldiers in the plateau were alcohol users.Conclusions Although the nutrition knowledge of plateau forces were poor, and the nutritional practices were influenced by nutritional knowledge, but the soldiers' attitude of changing unhealthy dietary habits was very positive. It is necessary to undertake the dietary nutrition education related to the high altitude nutrition among plateau forces to guild their nutritional practice scientifically.

Talus fracture is a relatively rare injury. The indication of surgical treatment of talus fracture is the obvious displacement of talus neck or talus body fracture, the purpose of which is to achieve anatomical reduction of the fracture site. The anatomical structure of talus is special, most of its surface is covered by the articular surface, so the injury often involves the articular surface, so the treatment is difficult and the incidence of complications after fracture operation is high, which is prone to ischemic necrosis and traumatic arthritis. Based on the anatomical structure of talus, we reviewed the latest treatment methods of talus fracture and its complications, the advantages and disadvantages of different treatment methods and the existing problems.

N-glycosylation modification, one of the most common protein post-translational modifications, occurs in heat shock protein gp96. The purpose of this study is to investigate the effect of N-glycosylation modification on immunologic function of the recombinant gp96 using the mutant gp96 in N-glycosylation sites. Firstly, wild-type and mutant gp96 proteins were expressed by insect expression system and their glycosylation levels were detected. To determine the effect of N-glycosylation on gp96 antigen presentation function, the IFN-γ+ CD8+ T cells in gp96-immunized mice and secretion level of IFN-γ were examined by flow cytometry and ELISA. The ATPase activity of gp96 was further detected by the ATPase kit. Finally, the effect of N-glycosylation on adjuvant function of gp96 for influenza vaccine was investigated in immunized mice. It was found that total sugar content of mutant recombinant gp96 was reduced by 27.8%. Compared to the wild type recombinant gp96, mutations in N-glycosylation sites resulted in decreased antigen presentation ability and ATPase activity of gp96. Furthermore, influenza vaccine-specific T cell levels induced by mutant gp96 as adjuvant were dramatically reduced compared to those by wild type recombinant gp96. These results demonstrate that N-glycosylation modification is involved in regulation of ATPase activity and antigen presentation function of gp96, thereby affecting its adjuvant function. The results provide the technical bases for development of gp96- adjuvanted vaccines.
Adjuvants, Immunologic ; Animals ; CD8-Positive T-Lymphocytes/metabolism* ; Glycosylation ; Heat-Shock Proteins ; Influenza Vaccines ; Mice