Objective To investigate the application value of ganglioside treated for neonatal hypoxic-ischemic encephalopathy (HIE).Methods The 88 neonatal HIE children were divided into observation group and control group according to random digits table method with 44 cases each.The children in the two groups were given general comprehensive treated measures.The children in observation group were treated with ganglioside.The children in control group were treated with citicoline.The clinical treatment situation,clinical symptoms situation,neonatal behavioral neurological assessment (NBNA) score situation,the changes of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in the two groups were compared and analyzed.Results The total efficiency in observation group was 90.91％(40/44),in control group was 68.18％ (30/44),there was statistically significant (P ＜ 0.01).The times of reflex recovery,muscle tension recovery and consciousness recovery in observation group were (7.04 ± 1.28),(8.13 ± 1.42) and (5.08 ± 0.90) d,in control group were (10.16 ± 1.57),(10.98 ± 1.33) and (9.76 ± 1.18) d,the targets in observation group were significantly shorter than those in control group (P ＜ 0.05).The change of NBNA score before and after treatment was (10.91 ± 1.83) scores in observation group,which was higher than that in control group [(9.07 ± 1.69) scores],and there was significant difference between the two groups (P＜ 0.05).The change ofTNF-α and IL-6 levels before and after treatment in observation group were (65.78 ± 12.25)and (88.58 ± 18.01) ng/L,which were lower than those in control group [(30.10 ± 11.93) and (32.69 ±17.94) ng/L],and there were significant differences between the two groups (P ＜0.05).Conclusions Ganglioside can be used as an ideal therapeutic drug for neonatal HIE to clinical application.Its mechanism may be related to the reduction of TNF-α and IL-6 levels.

Objective To investigate the significance of total bilirubin/albumin in predicting bilirubin neurotoxicity.Methods Eighty-three cases with hyperbilirubinemia who treated from May 2007 to August 2010 were selected,the serum total bilirubin and albumin were detected and total bilirubin/albumin was calculated.According to brainstem auditory evoked potential(BAEP)results,the patients were divided into normal BAEP group and abnormal BAEP group and compared.Results There were 27 cases of abnormal BAEP group and 56 cases of normal BAEP group.Total bilirubin and total bilirubin/albumin in abnormal BAEP group were higher than those in normal BAEP group[(356.50±59.23)μmol/L vs.(318.70±55.12)μmol/L,(5.02±0.49)×10-3 vs.(4.56±0.43)×10-3],the differences were significant (P<0.05).Multiple regression analysis showed:abnormal BAEP was closely related to total bilirubin/albumin(r=0.72,P<0.05),whih abnormal BAEP was not obviously related to total bilirubin(r=0.19,P>0.05).Conclusion Total bilirubin/albumin can reflect serum unconjugated bilirubin level of neonatus with hyperbilirubinemia better than total bilirubin,and it can can be taken as one index to evaluate the risk factors of bilirubin neurotoxicity.

The efficacy of growth hormone (GH) treatment in short children with small for gestational age (SGA) was evaluated and the association of serum insulin-like growth factor-1 (IGF) and IGF-binding protein-3 (IGFBP-3) levels with height increments was investigated.Fifty-five short children with SGA in our hospital from Sep 2009 to Sep 2013 were collected.Before GH treatment, insulin and arginine stimulation test were performed.The children were treated with recombinant GH with 0.15 U · kg-1 · d-1 for at least 12 months.After GH treatment, the height was (120.5 ± 17.63) cm, height SDS was (-1.53 ± 1.04) SDS, HtSDS was-2.04 ± 0.47, bone age was (6.19 ± 2.34) year, growth velocity was (7.24 ± 1.19) cm/year, all these parameters were significant higher than those before GH treatment.Pre-treatment serum levels of IGF-Ⅰ and IGFBP-3 were negatively correlated with post treatment growth velocity(r =-0.37 and r =-0.31;P =0.004 and P =0.021).GH treatment could effectively improve the height of short children with SGA.Children with lower pre-treatment serum levels of IGF-Ⅰ and IGFBP-3 may reach the higher growth velocity.

Objective To explore the application of Oxybuprocaine Hydrochloride Gel in colonoscopy examination. Methods 1000 patients received colonoscopy examination were randomly divided into experimental group and control group, 500 cases in each. Oxybuprocaine Hydrochloride Gel was applied on anal region in the experimental group before endoscopy for perianal anesthesia and lubrication. Paraffin oil was used in the control group to lubricate perianal and enteroscopy. The success rate of primary insertion, visual analogue pain score (VAS) score, examination time and postoperative complications were compared between the two groups. Results In the experimental group, the success rate of primary insertion (95%) was higher than that of the control group (76%), and the pain score was lower than that of the control group.The examination time was shortened, and the difference was statistically significant (P < 0.05). Conclusions Oxybuprocaine Hydrochloride Gel applied to colonoscopy can effectively reduce the patient's pain and discomfort, improve the success rate of primary insertion, shorten the examination time. And the method is worthy of clinical popularization and application.

Objective To study the effect of minimal enteral nutrition on the immune system of newborn infants under mechanical ventilation (MV) with intratracheal intubation.Methods Eighty-one patients under MV with intratracheal intubation were divided into two groups:treatment group and control group.Forty patients in treatmet group accepted early minimal enteral nutrition,while other 41 patients in control group accepted normal feeding,then T-lymphocytes (CD3,CD4,CD8),CD4/CD8,immunoglobulins (IgG,IgA,IgM),occurane of secondary infection,time of beginning fully enteral feeding,occurance of abdominal distension were recorded.Results There were no significant differences of T-lymphocytes (CD3,CD4,CD8),CD4/CD8,immunoglobulins (IgG,IgA,IgM) changes between the two groups in which the time of patients beginning MV under intratracheal intubation was less than five days (P ＞ 0.05) ; while comparing patients in the two groups whose MV time was equal or greater than five days,the changes of T-lymphocytes CD3[(37.3 ± 1.6)％,(42.6 ±2.8)％],CD4[(29.1 ±1.7)％,(34.6 ±2.1)％],CD4/CD8 (17.9 ±1.8,18.9 ± 1.6),immunoglobulins IgA[(55.42 ±3.98) mg/L,(129.00 ±7.76) mg/L],IgM[(130.29 ±10.92) mg/L,(317.33 ± 11.64) mg/L] in treatment group were more significant than those in control group(P ＜0.05) ;furthermore the differences of the secondary infection occurance,the time beginning fully enteral feeding,intravenous nutrition and hospital stays in the treatment group [20％ (8/40),(8.56 ± 1.78) d,(10.56 ± 1.78) d,(12.63 ± 1.73) d] were lower significantly than those in the control group [48.78％ (20/41),(10.20 ± 1.65) d,(12.15 ± 1.69) d,(14.15 ± 1.64) d] (P ＜ 0.05).These were no significant differences on abdominal distension and gastric retention between the treatment group [20％ (8/40),17.5％ (7/40)]and the control group [24.39％ (10/41),19.51％ (8/41)] (P ＞ 0.05).Conclusion Early minimal enteral nutrition in infants under long time MV with intratracheal intubation could improve immune function recovery,reduce the occurance rate of secondary infection,shorten the time beginning fully enteral feeding,intravenous nutrition and hospital stay.

Objective:To investigate the efficacy and safety of noninvasive high-frequency oscillatory ventilation in the treatment of respiratory failure in premature infants.Methods:Sixty-one premature infants with respiratory failure who received treatment in Neonatal Intensive Care Unit, Wenzhou Central Hospital from June 2019 to June 2020 were included in this study. They were randomly assigned to undergo either noninvasive high-frequency oscillatory ventilation (observation group, n = 31) or transnasal continuous positive airway pressure ventilation (control group, n = 31). Blood gas indicators, including pH value, oxygen partial pressure (PaO 2), partial pressure of carbon dioxide (PaCO 2), oxygenation index (OI), and respiratory index (RI), were determined before ventilation and 2, 8, 12, and 24 hours after ventilation. At the same time, the incidence of complications post-ventilation, including intracranial hemorrhage, bronchopulmonary dysplasia, air leakage, nasal crush injury, retinopathy of prematurity, abdominal distension, and necrotizing enterocolitis, was recorded in each group. Results:There were no significant differences in pH value, PaO 2, PaCO 2, OI, and RI pre-ventilation between observation and control groups ( t = 0.58, 0.64, 0.85, 0.43, 0.70, all P > 0.05). pH value, PaO 2, and OI measured 2, 8, 12 and 24 hours post-ventilation were significantly higher in the observation group than in the control group (pH value: t2 h = 3.20, t8 h = 4.81, t12 h = 6.39, t24 h = 5.22; PaO 2value:t2 h = 5.80, t8 h = 6.31, t12 h = 7.55, t24 h = 6.97; OI value: t2 h = 6.38, t8 h = 8.37, t12 h = 11.30, t24 h = 9.61). PaCO 2 and RI values were significantly lower in the observation group than in the control group (PaCO 2value:t2 h = 4.29, t8 h = 5.09, t12 h = 6.83, t24 h = 8.30; RI value: t2 h = 3.26, t8 h = 5.81, t12 h = 7.36、 t24 h = 6.54) (all P < 0.05). The incidence of complications was significantly lower in the observation group than in the control group [25.81% (8/31) vs. 46.67% (14/30), χ2 = 8.75, P < 0.05]. Conclusion:Compared with transnasal continuous positive airway pressure ventilation, noninvasive high-frequency oscillatory ventilation leads to better ventilation performance and a greater oxygenation ability, exhibits stronger effects on CO 2 clearance and acidosis correction, and has fewer complications in premature infants with respiratory failure. Therefore, noninvasive high-frequency oscillatory ventilation is suitable for clinical application.