Objective To evaluate the effect of anti-rejection of glucocorticoid-free immunosuppressive regimen on allogenic islet transplantation.Methods Tacrolimus (FK506)+ mycophenolate mofetil (MMF) and FK506+MMF+prednisone (Pred) were administered respectively for two weeks to inhibit rejection after allogenic islet transplantation in rats,which were compared with control group. The concentrations of blood glucose,insulin and C-peptide were determined dynamically in recipients and the sites of transplantation were observed morphologically.Results As compared with control group without immunosuppressive agents,FK506+MMF and FK506+MMF+Pred could prolong the survival time of grafts significantly. There were many morphologically intact islets in liver of recipients two months after transplantation. Group FK506+MMF kept normal blood glucose,insulin and C-peptide beyond 60 days after transplantation,but compared with the former,group FK506+MMF+Pred secreted less C-peptide ( P

Objective:To evaluate intestinal stenting in patients of left colon cancer complicated with acute intestinal obstruction and analyze its short-term and long-term efficacy.Methods:Sixty patients who suffered from left colon cancer complicated with acute intestinal obstruction at the First Department of General surgery, Chengdu Second People's Hospital from Sep 2016 to Sep 2019 were retrospectively enrolled. According to treatment methods, the patients were divided into the stent implantation staged surgery group (24 patients ) and the control group receiving emergency surgery (36 patients).Results:There was no significant difference in perioperative course between the two groups. There was no significant difference in three year overall survival rate or recurrence and metastasis rate between the two groups. Preoperative bowel preparation rate in stent implantation group was obviously higher than the control group (100% vs. 44%, P=0.000). The laparoscopic surgery rate of the stent implantation group was higher than the control group (21% vs. 0, χ2=9.857， P=0.002). The enterostomy rate of the stent implantation group was lower than the control group (0 vs. 19%, χ2=10.237， P=0.005). There was a higher rate of one-stage excision and anastomosis in the stent implantation group than in the control group (100% vs. 81%, χ2=7.760， P=0.005).There was a higher lymph nodes deissection rate in the stent implantation group than in the control group, [11(7-14) vs. 6(3-10), Z=-2.672， P=0.006]. Conclusions:For patients who suffered from left colon cancer complicated with acute intestinal obstruction, the intestinal stent can improve the bowel preparation to a greater extent, which can increase the proportion of one-stage anastomosis and reduce enterostomy rate.