Objective To observe the expression of heparan sulfate proteoglycan(HSPG)-Agrin in rat cortex of postnatal time in order to understand the relation between the development of cortex and agrin. Methods We performed immunohistochemical analysis of agrin expression in the postnatal rat cortex with agrin-specific polyclonal antibody. Results Agrin expression was shown to have significant differences in the different periods of postnatal time.It increased from postnatal 2 day(p2) to postnatal day 6(p6),then declined steadily after p6 and reached adult levels by 4w.In this periold of time,agrin has different expression peak following to the cortex layer development.Conclusion:Agrin is related to the development of cortex and may play an important role in the developing of central nervous system.;

ObjectiveTo explore the possibility of repairing sciatic nerve gap of rats with artifical nerve graft.MethodsA novel artifical nerve guide was developed and used to suture the 15 milimeter long right sciatic nerve gap of 10 rats, other 7 rats were the control with the right sciatic nerve gap alone.2 and 4 monthes after operation, immunohistochemistry, Osmium staining, Bodian staining,motor end plate staining,WGA-HRP stain tracing have been done to observe the effect of repairing.Results2 months after operations, the sciatic nerve gap were repaired by the regeneration nerve.There was not evident inflammation in the defects.ConclusionsThe artifical nerve graft can induce the nerve to regenerate.

【Objective】 To investigate the relationship of replication factor C subunit 2 (RFC2) with the prognosis of glioblastoma (GBM) and cell proliferation, as well as its underlying molecular pathway in GBM development. 【Methods】 Using bioinformatics methods, cell cycle genes were screened as independent prognostic factors for GBM. Combined with clinical indicators, a risk scoring model for GBM patients was established and validated. The target gene RFC2 was analyzed with GO, KEGG, and GSEA. U87 GBM cells at logarithmic growth stage were transfected with lentivirus and divided into different groups (control, ShRFC2 #1, and shRFC2#2 groups). qRT-PCR, Western blotting, Edu staining, and cloning assay were used to detect mRNA expression, protein expression, and cell proliferation. 【Results】 The expression of RFC2 was upregulated in GBM and showed an obvious upregulation trend with the increase of pathological grade of glioma. The analyses of gene function and pathway indicated that RFC2 was involved in the processes of sister chromosome segregation, chromosome segregation, organelle fission, and mitosis by promoting the transition of G1 to S phase during cell cycle. qRT-PCR and Western blotting showed that compared with the control group, the amount of mRNA and translated protein in the knockdowned groups decreased (P<0.000 1). The positive rate of Edu staining and the colony forming ability decreased (P<0.000 1, P<0.001). 【Conclusion】 RFC2 is highly expressed in glioblastoma and associated with pathological grade of glioma and poor prognosis of patients. It also promotes the cell proliferation function of glioblastoma. RFC2 may be a potential biomarker and therapeutic target for glioblastoma.