OBJECTIVETo ascertain the optimal harvest time of Erigeron breviscapus.

METHODThe dry matter weight accumulation of different organs in growth process and contents of scutellarin and coffeic acid ester in whole plant of E. breviscapus were determined.

RESULTThe number of leaves per plant, the dried weight of single leaf and dry matter weight of whole plant and different organs reached the highest after seedling 130-140 d. The content of scutellarin gradually decreased with growth period, and sharply decreased after seedling 140 d. The content of coffeic acid ester varied irregularly with growth period.

CONCLUSIONThe optimal harvest time of E. breviscapus is in early bloom period after seedling 130 d.

Apigenin ; analysis ; Biomass ; Erigeron ; chemistry ; growth & development ; Gardening ; Glucuronates ; analysis

Two novel isocoumarins, erigeronone C (1) and D (2), were isolated from the ethanol extract of the whole plant of Erigeron breviscapus (Vant.) Hand.-Mazz (Compositae). Their structures were respectively elucidated as 8, 9-dihydroxypyrano [3, 2-c] isochromen-4, 6-dione (1) and 4, 7-dihydroxy-3-(3-hydroxy-4-oxo-4H-pyran-2-yl)-1H-isochromen-1-one (2) on the basis of spectral analyses. Both structures of 1 and 2 possess a gamma-pyrone moiety and that is rare in natural products.
Erigeron ; chemistry ; Isocoumarins ; chemistry ; isolation & purification ; Molecular Structure ; Plants, Medicinal ; chemistry

Dengzhan Shengmai Capsules( DZSMC),a well-known traditional Chinese medicine( TCM) formula,is comprised of the main drug of Erigeron breviscapus,and supplemented with Panax ginseng,Ophiopogon japonicus and Schisandra chinensis,with functions of supplementing Qi and nourishing Yin,promoting blood circulation and strengthening brain. DZSMC is the only Chinese patent drug with A-level evidence-based medicine in secondary prevention for stroke and ranks first among TCMs for neurological treatment. Modern studies indicate that the chemical constituents of DZSMC mainly include flavonoids,phenolic acids,lignans,saponins and so on. Pharmacological experimental studies have shown that DZSMC has such pharmacological effects as anti-oxidation,anti-inflammatory and anti-myocardial ischemia. DZSMC is mainly used in the convalescent care of ischemic cardiovascular and cerebrovascular diseases,and is often used in combination with various conventional therapeutic drugs to exert clinical efficacy through brain protection,neuroprotection,etc.,and improve clinical symptoms in patients. In this review,according to domestic and international related literature combined with research results obtained by our project,the research advances in the chemical constituents,pharmacological effects and clinical application of DZSMC have been systematically reviewed and summarized,providing reference and support for further study and secondary development of the formula.
Drugs, Chinese Herbal/pharmacology* ; Erigeron/chemistry* ; Humans ; Medicine, Chinese Traditional ; Ophiopogon ; Panax ; Phytochemicals/pharmacology* ; Phytotherapy ; Schisandra

OBJECTIVETo establish the fingerprint detecting standard of xinshao Injection by reversed phase high performance liquid chromatography.

METHODThe chromatographic conditions were as follows: an Diamonsil C18 column was used, the mobile phase was composed of 80% acetontrile and 0.1% phosphoric acid with gradient elution, the flow rate 1.0 mL min(-1), column temperature was 40 degrees and the UV absorbance detection was set at 230 nm.

RESULTA standard HPLC fingerprint procedure was developed for xinshao Injection, with 8 common peaks. Under the same selected chromatographic conditions, the HPLC fingerprints of raw drugs and their intermediate, finished products were obtained with good separation and correlation (no less than 0.97) according to the technical requirements of fingerprint on Injection of Chinese traditional medicine.

CONCLUSIONThis method was accurate, repeatable and can be used as the important parameters of the quality control for xinshao injection.

Chromatography, High Pressure Liquid ; methods ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; Erigeron ; chemistry ; Injections ; Paeonia ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Reproducibility of Results

OBJECTIVEA HPLC-ECD method was established to determine scutellarin in rat bile.

METHODThe analytical column was Prontosil C18 (4.6 mm x 250 mm, 5 microm), and the mobile phase was consisted of methanol, 50 mmol x L(-1) phosphate buffer (adjusted by phosphoric acid to pH 2. 6), and tetrahydrofuran (40: 60: 10) , the flow rate was 1.0 mL x min(-1); the potential electrode voltage was 100 mv.

RESULTConcentration profile of scutellarin in rat bile was shown in this paper after oral administration of scutellarein.

CONCLUSIONOnly scutellarin was detected in rat bile, while both of scutellarin and scutellarein were detected in rat plasma.

Animals ; Apigenin ; analysis ; blood ; isolation & purification ; Bile ; chemistry ; Chromatography, High Pressure Liquid ; Erigeron ; chemistry ; Glucuronates ; analysis ; Male ; Plants, Medicinal ; chemistry ; Rats ; Rats, Sprague-Dawley

OBJECTIVEA high-performance liquid chromatographic (HPLC) method was developed for simultaneous determination of chlorogenic acid, scutellarin, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid in different parts of Erigerontis Herba.

METHODThe four constituents were measured on an Agilent Zorbax SB-C18 column (4.6 mm x 450 mm, 5 microm) with a gradient elution of acetonitrile (A) -0.3% phosphoric acid solution (B) (0-10 min, 12%-15% A, 10-32 min, 15% A, 32-33 min, 15%-20% A, 33-50 min, 20%-22% A) at wavelength of 335 nm and 327 nm, and a flow rate of 1.0 mL x min(-1) and the column temperature was 30 degrees C.

RESULTLinearity of each standard was established in the concentration range of 0.050 1-1.002 microg for chlorogenic acid, 0.165 9-3.318 microg for chlorogenic acid, 0.049 7-0.994 microg for 3,5-dicaffeoylquinic acid, 0.048 7-0.974 p.g for 4,5-dicaffeoylquinic acid respectively, with correlation coefficient r > 0.999 6. Average recoveries (n = 6) of 4 compounds were 98.53% with a RSD of 0.94%, 99.68% with a RSD of 0.49%, 98.78% with a RSD of 1.1%, 99.06% with a RSD of 0.81%, respectively.

CONCLUSIONThe developed method is simple, accurate, and precise, it can be used for the quantitative analysis of Erigeron breviscapus.

Apigenin ; analysis ; chemistry ; Chlorogenic Acid ; analogs & derivatives ; analysis ; chemistry ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; Erigeron ; chemistry ; Glucuronates ; analysis ; chemistry ; Quinic Acid ; analogs & derivatives ; analysis ; chemistry

Dengzhanxixin injection is extracted from herbs of Erigeron breviscapusis. Its function includes activate blood, dispel stasis, unblock the collaterals and relieve pain. In clinical, it is widely used for static blood obstruction, wind-stroke and hemiplegia, numbness of limbs, deviated eyes and mouth, dysphasia, chest impediment, heart pain, ischemic stroke, coronary heart disease, and angina pectoris with the pattern mentioned above. In this paper, we planed to review the pharmacological and toxicological effects of Dengzhanxixin injection from relevant studies.
Animals ; Coronary Disease ; drug therapy ; Drug Therapy ; Drug-Related Side Effects and Adverse Reactions ; Drugs, Chinese Herbal ; adverse effects ; pharmacology ; Erigeron ; chemistry ; Humans ; Stroke ; drug therapy

OBJECTIVETo investigate the neuroprotective effects of the constituents in Herba Erigerontis on neuroblastoma SH-SY5Y cells, and find out its possible material foundation in treating Alzheimer's disease (AD).

METHODDifferent combinations of the three constituents in Herba Erigerontis were prepared according to the orthogonality experiment, and the indexes (MTT reduction assay, lipid peroxidation and expressions of nAChR alpha7 protein)were observed upon the SH-SY5Y cells followed by treatment of these combinations and beta-amyloid peptide (AP). The pharmacology data thus obtained and peak data in UPLC fingerprint were analyzed through ANOVA and correlationship by SPSS to give the information of active possible material foundation.

RESULTConstituents B and C showed clear activity and peaks of 4, 7-12 did positive correlationship according to the correlation of fingerprints and pharmacology.

CONCLUSIONThis study makes a valid approach for deducing the active constituents even the exact compounds against neurotoxicity induced by Abeta by correlation of fingerprints and pharmacology.

Alzheimer Disease ; drug therapy ; Amyloid beta-Peptides ; toxicity ; Analysis of Variance ; Animals ; Cell Line, Tumor ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; therapeutic use ; Erigeron ; chemistry ; Humans ; Nervous System ; drug effects ; Neurotoxins ; toxicity

OBJECTIVETo study the chemical constituents from herbs of Erigeron breviscapus.

METHODThe compounds were isolated and purified by various chromatographic techniques. Their structures were identified on the basis of physicochemical properties and spectral analysis.

RESULTTwelve compounds were isolated and structurally identified as quercetin-3-O-beta-D-glucoside (1), 5, 7-dihydroxychromone (2), 3-O-caffeoyl-gamma-quinide (3), naringenin (4), 3, 5-di-O-caffeoylquinic acid (5), 3,4-di-O-caffeoylquinic acid (6), 4, 5-di-O-caffeoylquinic acid (7), 1,3-di-O-caffeoylquinic acid (8), 1, 5-di-O-caffeoylquinic acid (9), 3-O-caffeoylquinic acid (10), 4-O-caffeoylquinic acid (11) and chlorogenic acid (12).

CONCLUSIONCompounds 1-4 were isolated from this plant for the first time.

Chlorogenic Acid ; analysis ; isolation & purification ; Chromatography ; Chromones ; analysis ; isolation & purification ; Erigeron ; chemistry ; Flavanones ; analysis ; isolation & purification ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Plants, Medicinal ; chemistry ; Quercetin ; analogs & derivatives ; analysis ; isolation & purification ; Quinic Acid ; analogs & derivatives ; analysis ; isolation & purification

AIMTo establish RP-HPLC method for determination of plasma scutellarin concentration and study of the pharmacokinetic behavior of scutellarin in rat after ig administration of breviscapine and its beta-cyclodextrin complex (breviscapine-beta-CD).

METHODSMobile phase composed of methanol and acetate buffer. The column was Shim-pack C18. Twelve rats randomized into 2 groups were separately given breviscapine and breviscapine-beta-CD at single dose of 10.8 mg.kg(-1). Drug in plasma was extracted and determined by HPLC. The pharmacokinetic parameters were calculated by 3P97 software.

RESULTSLinearity was obtained over the range of 10-400 ng.mL(-1). The recovery was 95.32%-98.81%. C(max) and AUC(0-12 h) of breviscapine were (154 +/- 18) ng.mL(-1) and (710 +/- 126) ng.h.mL(-1). For breviscapine-beta-CD, C(max) and AUC(0-12 h) were (328 +/- 31) ng.mL(-1) and (1,093 +/- 200) ng.h.mL(-1), respectively. There were significant differences of AUC(0-12 h) between breviscapine and breviscapine-beta-CD (P < 0.01).

CONCLUSIONThe assay method was suitable for the determination of scutellarin plasma concentration in rat. Brevescapine-beta-CD showed greater absorption compared with that of brevescapine.

Animals ; Area Under Curve ; Chromatography, High Pressure Liquid ; methods ; Drug Compounding ; Erigeron ; chemistry ; Flavonoids ; blood ; isolation & purification ; pharmacokinetics ; Male ; Plants, Medicinal ; chemistry ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; beta-Cyclodextrins ; blood ; pharmacokinetics