Objective:To evaluate the safety and effectiveness of air bubble-assisted intraocular lens (IOL) implantation and its effect on corneal endothelial cell during phacoemulsification for age-related cataract.Methods:A non-randomized controlled study was conducted.One hundred and seventy eyes of consecutive 170 patients with age-related cataracts who received phacoemulsification combined with one-stage IOL implantation were included from March 2019 to September 2019 in First Affiliated Hospital of Zhejiang Chinese Medicine University in this study.Among the operative eyes, 92 eyes that underwent phacoemulsification combined with air bubble-assisted IOL implantation served as air bubble-assisted group and 78 eyes that received hyaluronan-assisted IOL implantation served as hyaluronan-assisted group.All the subjects were followed up for successive 12 weeks.The total operative duration and IOL implantation duration, corneal endothelial cell count (ECC) and the proportion of hexagonal cells before and 1, 4, 12 weeks after surgery, and intraocular pressure (IOP) before and 1 day, 1 week and 4 weeks after surgery were compared between the two groups.And the total surgery time and IOL implantation time were compared between the two groups.This study adhered to the Declaration of Helsinki and was approved by an Ethics Committee of First Affiliated Hospital of Zhejiang Chinese Medicine University (No.KL-010-01).Results:The operation went well in all the eyes and there was no serious intraoperative or postoperative complication in all the operative eyes.There was no significant difference in preoperative and postoperative ECC, the proportion of hexagonal cells and the IOP between the two groups ( Fgroup=0.004, P=0.953; Fgroup=0.706, P=0.042; Fgroup=1.896, P=0.170). The postoperative ECC and the proportion of hexagonal cells at different time points were significantly smaller than the preoperative ECC and hexagonal cells proportion in the two groups (all at P<0.05). The IOP was elevated in 2 eyes in the hyaluronan-assisted group one day after operation, which returned to normal after anterior chamber puncture.There was no postoperative high IOP in the air bubble-assisted group.The total surgery time and IOL implantation time were (357.78±36.07) seconds and (50.63±8.37) seconds respectively in the hyaluronan-assisted group, which were significantly longer than (256.95±47.74) seconds and (29.66±6.58) seconds respectively in the air bubble-assisted group, showing significant differences between the two groups (both at P<0.01). Conclusions:Air bubble-assisted technique can shorten the IOL implantation duration.Both air bubble-assisted and hyaluronan-assisted IOL implantation causes no additional risk to corneal endothelium.

Objective To investigate a kind of new operation style aiming directly at middle and late stage cardic cancer. Methods To use the routine resection and retrograde resection simultaneusly as surgical treatment for those difficult to be exercised. Results This is no exploration case in this group. There is no case perioperation death. This method has improved the patient's epitite and quality of life (QOL)obviously. Conclusion This operation style provided a new kind of effective method for the treatment of middle and late stage cardic cancer. It set a solid foundation for improving the patient's QOL and combined treatment after the operation.

In this paper, scientific theory was used to prove that the meridian was the resonant channel of information energy of life, and experiment and demonstration was performed to reveal meridians to explore the significance of revealing meridian. Based on the theory of physics, biochemistry, molecular biology and information theory, the essence of meridian was demonstrated in theory. According to TCM meridian theory, acupuncture was applied at selected acupoints; the infrared thermograms of stomach meridian of foot , large intestine meridian of hand , small intestine meridian of hand , pericardium meridian of hand , heart meridian of hand , triple energizer meridian of hand , liver meridian of foot , bladder meridian of foot were recorded before and after acupuncture. As a result, thermogram recorded and showed the visual image of the meridian. It is indicated that an energy transmission network which cannot be dissected existing in the body, which was believed to be meridian. It was the resonant channel of information energy of life, and could be shown by thermogram. These experiments and theory have significance in science.
Acupuncture ; Acupuncture Points ; Humans ; Meridians

RNAi technology has aroused wide public interest due to its high efficiency and specificity to treat multiple types of diseases. However, the effective delivery of siRNA remains a challenge due to its large molecular weight and strong anionic charge. Considering their remarkable functions and features that are often desired in drug delivery carriers, biomimetic systems for siRNA delivery become an effective and promising strategy. Based on this, covalent attachment of synthetic cell penetrating peptides (CPP) to siRNA has become of great interest. We developed a monomeric covalent conjugate of low molecular weight protamine (LMWP, a well-established CPP) and siRNA a cytosol-cleavable disulfide linkage using PEG as a crosslinker. Results showed that the conjugates didn't generate coagulation, and exhibited much better RNAi potency and intracellular delivery compared with the conventional charge-complexed CPP/siRNA aggregates. Three different synthetic and purification methods were compared in order to optimize synthesis efficiency and product yield. The methodology using hetero-bifunctional NHS-PEG-OPSS as a crosslinker to synthesize LMWP-siRNA simplified the synthesis and purification process and produced the highest yield. These results pave the way towards siRNA biomimetic delivery and future clinical translation.

In this paper, we prepared a dual functional system based on dextrin-coated silver nanoparticles which were further attached with iron oxide nanoparticles and cell penetrating peptide (Tat), producing Tat-modified Ag-FeO nanocomposites (Tat-FeAgNPs). To load drugs, an -SH containing linker, 3-mercaptopropanohydrazide, was designed and synthesized. It enabled the silver carriers to load and release doxorubicin (Dox) in a pH-sensitive pattern. The delivery efficiency of this system was assessed using MCF-7 cells, and using null BalB/c mice bearing MCF-7 xenograft tumors. Our results demonstrated that both Tat and externally applied magnetic field could promote cellular uptake and consequently the cytotoxicity of doxorubicin-loaded nanoparticles, with the IC of Tat-FeAgNP-Dox to be 0.63 µmol/L. The delivery efficiency of Tat-FeAgNP carrying Cy5 to the mouse tumor was analyzed using the optical imaging tests, in which Tat-FeAgNP-Cy5 yielded the most efficient accumulation in the tumor (6.7±2.4% ID of Tat-FeAgNPs). Anti-tumor assessment also demonstrated that Tat-FeAgNP-Dox displayed the most significant tumor-inhibiting effects and reduced the specific growth rate of tumor by 29.6% ( = 0.009), which could be attributed to its superior performance in tumor drug delivery in comparison with the control nanovehicles.