BACKGROUND:Many experiments have demonstrated that tissue engineering scaffolds prepared by polymer materials alone or biomaterials cannot meet the requirement of tissue engineering research.
OBJECTIVE:To evaluate biological characteristics and cel affinity of poly(hydroxybutyrate-co-hydroxyoctanoate)/col agen composite scaffold.
METHODS:Tissue engineering scaffolds were prepared by combination of poly(hydroxybutyrate-co-hydroxyoctanoate) and col agen at different proportions (2%, 4%, 6%, 8%and 10%) using solvent casting/particulate leaching method. Inner structure and apertures were observed by scanning electron microscope, and the porosity was determined by liquid displacement method. Rabbit chondrocytes were co-cultured with poly(hydroxybutyrate-co-hydroxyoctanoate)/col agen composite scaffold and poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold. Growth curve of cel s was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cel adhesion on the scaffolds was observed by scanning electron microscope.
RESULTS AND CONCLUSION:The pore size and porosity of the composite scaffold were about 200μm and (85±2)%, respectively. The cel affinity dynamical y increased with the increasing of proportion of col agen. Compared with the poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold, the poly(hydroxybutyrate-co-hydroxyoctanoate)/col agen composite scaffolds are better to improve cel adhesion and proliferation, with favorable cel ular affinity.

Objective　We aimed to analyze the gender differences by evaluating the motor and non-motor symptoms among the patients with parkinson’s disease and the patients with depression of parkinson’s disease，and to explore the possible pathogenesis further and make sex-specific therapeutic strategies. Methods　Two hundred patients with parkinson’s disease from both the outpatients department and the ward were included in this analysis. There were 56 patients whose HAMD scores exceeded 19，which were divided into the depession of parkinson’s disease （dPD） group。There are 144 patients whose HAMD scores under 20，which were divided into the non-depession of parkinson’s disease （ndPD） group. The scores from all the scales were analyzed using statistical software. Results　①The depression incidence of females was significantly higher than that of males in PD patients （P<0.05）. ②Rigidity score and Bradykinesia score in males were significantly higher than that in females in dPD patients （P<0.05）. Tremor score in males was significantly lower than that in females in dPD patients （P<0.05）. ③A significantly higher score in mood/apathy and feel problem/hallucination domains were observed in female PD patients （P<0.05）. ④The HAMA score，HAMD score，NMSQ total score of dPD patients were all significantly higher than that of ndPD patients （P<0.05）. The PDSS score of dPD patients was significantly lower than that of ndPD patients （P<0.05）. The tremor score，rigidity score，postural instability score，bradykinesia score，UPDRS-III score of dPD patients were all significantly higher than that of ndPD patients （P<0.05）. ⑤The HAMD scores correlated with levodopa equivalent doses （Led） （r=-0.31，P=0.01），H-Y stage （r=0.23，P=0.03），PDSS （r=0.36，P=0.01） and rigidity （r=0.27，P=0.04） scores. Conclusion　①There are gender differences bewteen males and females in motor disorders in depression of parkinson’s disease. ②The motor disorders and non-motor disorders were more severe in depression of parkinson’s disease than in non-depression of parkinson’s disease. ③The HAMD score in depression of parkinson’s disease correlated with levodopa equivalent doses，the motor disorders and the non-motor disorders. ④The spectrum of motor and non-motor symptoms showed different gender distribution in PD and dPD patients. Gender-specific therapeutic agents hold particula attention for developing treatments with optimal efficaty in men and women in the future.

Objective To analyze the accuracy and influencing factors of miniprobe ultrasonic endoscope in evaluating submucosal infiltration of colorectal laterally spreading tumor(LST).Method A retrospective analysis was conducted on the clinical data of 213 patients(268 lesions in total)with colorectal LST who underwent endoscopic submucosal dissection(ESD)treatment from June 2018 to August 2021.We summarized the clinical pathological characteristics and miniprobe ultrasonic endoscope examination results of LST,then analyzed the accuracy of miniprobe ultrasonic endoscope examination and the risk factors affecting the accuracy of miniprobe ultrasonic endoscope examination.Results The accuracy rate of miniprobe ultrasonic endoscope examination was 93.28%,and there was a statistically significant difference in the accuracy rate of miniprobe ultrasonic endoscope examination between different lesion surface morphologies(P = 0.000).Multivariate Logistic regression analysis showed that mix-ed nodule and false depression lesions were risk factors for inaccurate EUS assessment.Conclusion Colorectal LST is a special type of tumor,and miniprobe ultrasonic endoscope examination has a high accuracy in evaluating its infiltration depth.The surface morphology of the lesion is a risk factor that affects the accuracy of miniprobe ultrasonic endoscope examination.When the lesion is a nodule mixed type or pseudo depressed type,it can easily lead to inaccurate miniprobe ultrasonic endoscope examination.

OBJECTIVE To explore the influence of surgical history on chemotherapy -induced nausea and vomiting （CINV）. METHODS A retrospective case -control study was adopted ，with 824 patients undergoing chemotherapy as the object . A total of 27 items were collected ，including demographic data ，medical history data ，pre-chemotherapy data ，and chemotherapy treatment status. Logistic regression model was used to analyze the relationship between the history of surgery and the risk of CINV . The multiple models were constructed to correct potential confounding factors ，and subgroup analysis was performed on patients with surgical history . RESULTS The incidence of CINV was higher in patients with surgical history . The statistical result before adjustment was [OR＝1.72，95%CI（1.31，2.28），P＜0.001]；after adjusting potential confounding factors ，the statistical result was [OR＝1.78，95% CI（1.28，2.48），P＝0.001]. In the subgroup analysis ，the time between surgery and chemotherapy was different ， and the impact of surgical history on CINV was different ，and the results were statistically significant （P＝0.027）. The risk of CINV showed decreasing trend with the time ，and the results were statistically significant （P for trend ≤0.050）. Compared with patients who had not undergone surgery ，patients who had undergone surgery within one year had a higher risk of CINV [OR＝ 2.33，95%CI（1.52，3.59），P＜0.001]. CONCLUSIONS Patients with surgical history are more prone to CINV ，and the risk of CINV shows a downward trend in the length of time from surgery .