Background: Development and progression of cancer is accompanied by different morphological and functional changes of cells. One of the most important changes is the expression and activity of enzymes in the cellular fatty acids metabolism that reflects in cell membrane lipid composition and increases fluidity of cancer cell membrane. The Ellipin, prepared from bovine liver, is a newly developed anticancer agent containing several important fatty acids.Goal: To investigate effects of Ellipin on hepatic cancer cell function such as proliferation, migration and adherent activity and apoptosis of cancer cell lines in vitro.Materials and Methods: This study was conducted in the Laboratory of Molecular Biology, Institute of Biology, MAS. The Ellipin was developed in the Drug Research Institute of Monos group. HepG2, HCC, 23132/87, MDCK cells lines and the primary liver cancer cells (PCC) were used for proliferation assay. Only HepG2 cell line was used for MTT, Migration, Spreading and Apoptosis assays.Results: The results of proliferation assay showed that the ellipin decreased the proliferation activity of HepG2 and PCC cells depending on concentrations; in 50μg/ml 2-3 times, 250μg/ml fully stopped cells divisions. The Ellipin reduced mitochondrial reeducates enzyme activity of HepG2 cells depending on its concentrations. For example, in 50μg/ml ellipin concentration case, the number of alive cells decreased 2 times. The migration of HepG2 cells treated with 100μg/ml ellipin was decreased by 22.3% compared to the control cells. Also the number of adhered cells was reduced by Ellipin treatment. After 50μg/ml, 100μg/ml, 250μg/ml ellipin treatment, the number of apoptic cells were 14,6%, 45,6%, 100% of initial culture cells, respectively.Conclusions: Our results showed that the Ellipin suppresses HepG2 cancer cell proliferation and decreases migration and spreading activities and also inducts the cell apoptosis.

BackgroundMonos Pharm LLC has been started production of Dentamon which is an elixir medicine for gumtissues and a oral cavity inflammation and consumer product has been under appreciated today since1998. Now days, as the technology develops, improved levels of consumer demand for consumptionand they want the product easier to use. In this study, sustainable refers to both the technology andstandardization characteristics of gel medicine for a new Dentamon or Dentos gels were prepared using20% ethanol extract for mixture of Chamaenerion angustifolium L, Stellera chamajasme L and Oxytropispseudoglandulosa which are pharmacological active for gum tissues and a oral cavity inflammation.GoalThe aim of this work was to standardize of Dentamon elixir gel medicine and make technological studyof Dentamon.Materials and MethodsThe present study included plant species which were Chamaenerion angustifolium L, Stellerachamajasme L and Oxytropis pseudoglandulosa. Those three medicinal plants were collected fromdifferent regions of Mongolia and samples their upper part of ground. The plants were used for thepurpose of their phytochemical analysis and technological study of gel formulation. For the contentof flavonoids, total coumarin and tannin in the gel and extract of those plants were determined byspectrophotometric method. The direct measurement of the microbiological climacteric was determinedin extract by according to Mongolian National Pharmacopeia and the viscosity property of gel medicinewas identified using viscometer.ResultsThis study has revealed the presence of photochemical considered as active medicinal chemicalconstituents. Chemical tests of the screening and identification of main active components in the plantsunder study were carried out in the ethanol extract (20, 40, 70%) and aqueous extract using generalextraction method. The tannin content of the upper part in water and three different concentrated ethanolextract was found to be (2.16±0.04%, 1.73±0.04%, 2.58±0.04% and 1.74±0.02%), respectively. Thetannin content of upper part in 40% of ethanol extract of the plants was 7.40±0.21% and coumarin contentwas 3.01+0.09% and the total flavanoids content were 0.70+0.03%. There were not detected Esherichiacoli, Salmonella, Pseudomonas aeruginosa and Staphylococcus aureus in plant extracts. The gelmedicine was prepared from concentrated plant extract using dispersion method and and gel formingmaterial selection using 0.5%, 1%, 1.5% and 2% of carbomer. The results from gel formulation assay,the 0.5% of the gel was turbid liquid state, and 1% of the gel was a colorless, clear liquid state, 1.5% gelwas colorless, created very clear and 2% gel was colorless but it was very dense. The pH condition ofthe 1% of Dentos gel was 7.6 and the viscosity property was 7400000 mPa/sec, the flavonoid contentwas 0.165%, the total coumarin content was 0.69 and Pseudomonas aeruginosa, Staphylococcusaureus, Enterobacteriaceae did not detected. Dentos 1% gel was compared its pharmacological trialwith Hi Ora gel which is produced by Himalaya LLC. On the treatment 14 days, Dentos gel more reduced45.9% of wound area index than Hi Ora gel.ConclusionThe 40% ethanolic extracts of the studied plants contained many bioactive chemical constituentsincluding alkoloids, flavonoids, tannin and coumarin. The 1.5% of carbomer was most effectivefor make a new Dentos gel and also new generated gel was most effective against Pseudomonasaeruginosa, Staphylococcus aureus, Enterobacteriaceae. The new generated gel was standardizedby its appearance, viscosity property and content of coumarin, alkaliod, flavonoids and microbiologicalpurity characteristics.

BackgroundThe works done during the research include: conducting photochemical study on the surface of the piece of land on which the medicinal herbs chosen as samples for the research grow; extracting dry infusion from plants; identifying main substance and antibacterial activity of dry infusion; conducting pilot pharmacological experiment with mineral samples.GoalBased on the results of these experiments, we aimed at finding out wound healing functions of Chelidonium majus. L, Stellerae chamaejasme.L etc, widely used in both conventional and modern medicine as well as of such minerals as Sinder, Zeolite, Tormohon, Baragshun after selecting from them.Materials and MethodsAlkaloid, a biologically active substance found in surface of plant land and dry infusion, was determined by titration method, alkaloids such as protopine and berberine by high performance liquid chromatography, coumarin and flavonoid by spectrophotometric method, the amount of infusible substance by scale method. The methodologies of Gatsura.N and Avtandilov.G.G were adopted to make artificial wound on skin and to heal a wound respectively.ResultsAs the research result shows, the surface of the area where Stellerae chamaejasme.L grow, contains 0.33% ±0.004 total coumarin, 0.19 % ±0.002 flavonoid and 37.14 % ±0.61 infusible substance while that of Chelidonium majus.L contains 0.19% ±0.003 total alkaloid, 0.12 % ±0.004 flavonoid, 0.09 % ±0.002 coumarin and 36.27%±0.74 infusible substance. After the infusion was extracted from Stellerae chamaejasme.L and Chelidonium majus.L through method of percolation with mixer, there were 16% and 14.5% dry substance remained in the infusions respectively. These figures were reduced to 12.6% and 11.4% after freezing them in -500C for 72 hours in dry freezing. The fact that total coumarin contained in dry infusion of Stellerae chamaejasme.L is 2.95% and protopine and berberine in that of Chelidonium majus.L, is 28 мг,% and 3.7 мг,% respectively meets the requirements for medicinal plant extracts. It was found during the pharmacological experiment of preparing 25% oil ointment composed of minerals including Sinder, Tormohon, Zeolite, Baragshun that zeolite was the highest effective mineral in healing a wound by wound index. Therefore, we chose zeolite as an ingredient of the wound ointment. Oil ointments of 6 variations with 5% and 10% content were prepared from the dry infusion as well as minerals of the study plants, and pilot pharmacological experiment was conducted on an experimental mouse that had an artificial wound. The result of the experiment proved that oil ointments with 5% content of Stellerae chamaejasme.L, Chelidonium majus.L and zeolite were more effective in healing the wound than others.ConclusionBy photochemical analysis, the chosen plants proved to be meeting the requirements for medicinal plants. Pilot pharmacological experiment showed that zeolite was the one that accelerated a wound healing process more efficiently than others. Oil ointments with 5% content of dry infusion and 5% of zeolite were effective in healing a wound quickly.

Introduction : Attention-Deficit/Hyperactivity Disorder (ADHD) is a disorder that occurs during childhood development, which presents with signs of reduced attention and hyperactivity [1]. Necessary nutrients, such as trace minerals, including manganese, iron, zinc, iodine, selenium, copper, and chromium, are associated with changes in neuronal function that can lead to adverse effects on behavior and learning [2]. In addition to these, social, emotional, behavioral problems, and cognitive impairments such as executive dysfunctions are common in ADHD [3]. Goal: To evaluate the hair elements and intelligence quotient in children with ADHD. Materials and Methods: This is a cross-sectional comparative study conducted at elementary schools of Ulaanbaatar city. All in all 60 children of both genders aged between 7-12 years old were included in the study. Children were divided into two groups as children with ADHD group and a control group. Each group had 30 children. For assessment of emotional Intelligence EQ-i:YV - Emotional Quotient Inventory: Youth Version (Bar-On & Parker, 2000; it ad. Sannio Fancello, & Cianchetti, 2012) was used. Scalp hair samples were randomly collected from approximately ten sites around both sides of posterior parietal eminences and external occipital protuberance. Samples were then packed at room temperature and submitted for laboratory analysis. The study was approved by the Research Ethics Committee of Mongolian National University of Medical Sciences (Reg. No. 2018/Д-10). Results: The IQ of children with ADHD group were 85.03±16.86 p<.0001 and the IQ of control group =108.9±21.22, p<.0001. We identified hair minerals such as Mg, Zn, Pb, Se, Mn. We have then compared to each group and normal ranges of ages. ADHD group and the control group had Pb concentration that was slightly higher and inversely Mg concentration was slightly lower (r=-0.502, p=.005). Concentration of Pb, IQ were directly opposite (r=-0.38, p=.03). Conclusion
1. IQ was lower in the ADHD group compared to control group 85.03±16.86 p<.0001, monitored group 108.9±21.22, p<.0001.
2. The group with ADHD had lower Mg, Zn, and higher Pb, Se, Mn (p<.0001). The IQ decreased when there was increased Pb and decreased Mg.