remains the most frequent transfusion-transmitted viral infection; thus, the term occulthepatitis B virus infection (OBI) was introduced. OBI is simply defined as serologically undetectable hepatitis Bsurface antigen (HBsAg-ve), despite the presence of circulating HBV DNA with or without the presence of HBVantibodies.GoalTo determine the prevalence of occult hepatitis B among blood donors and evaluate the presence of HBV DNAin HBsAg negative plasma samples.Materials and MethodsIt includes 16700 samples which donated in NCTM in Ulaanbaatar in 2013. We used to “triplex” PCR assay thatincluded the detect of hepatitis B virus HBV-DNA in addition HCV-RNA and HIV1/2-RNA for whom with absenceof serological markers of infection. The studies used molecular biology methods were performed with the help ofequipment (ROCHE COBAS S 201) and technology based on Real Time PCR (pool size: 6 donation) Then wechoose HBsAg negative, DNA positive samples and determined, anti-HBc and anti-HBs by serological methods,of ELISA Wantai HBc and HBs 3.0 tests.ResultsThe 14948 samples were detected serological negative in the total of 16700 samples. PCR test results show 35(0.23%) positive by HBV-DNA 29 (82.9%) of the 35 DNA positive blood donors were alone anti-HBc positive and3 (8.6 %) were anti-HBs, anti-HBc positive. 7(17.1%) were seronegative. Of the 35 OBI cases, 28 (80%) weredetected the first time they were screened for HBV DNA while 7 (20%) gave one more HBV PCR-nonreactiveresults before detection. Callback studies we determined 2 cases were pre-HBsAg window period.Conclusion:The prevalence of HBV DNA positive in HBsAg negative blood donors is found 0.2%. HBV NAT needs eitherextreme sensitivity or to be performed on individual donations to eliminate HBV DNA-containing units.

BACKGROUND. WHO experts had identified as 4 percentage of total mortality in our glob is due toharmful use of alcohol [1]. Prevalence of the mental and behavioral disorders due to use of alcohol isoccurred 13.6 percent among the population aged 15-65 years old, thereof 22.0% of man and 5.0%of women in the morbidity of Mongolia [2].Thus, study in clinical features in patients with mental and behavioral disorders due to use of alcoholand result of psychotherapy among patients are required in this field.OBJECTIVE: Identifying of the result of psychotherapy and study on withdrawal features amongpatients with mental and behavioral disorders due to use of alcohol is proposed objective of thisclinical research.MATERIALS AND METHODS: In first stage, researchers had been used AUDIT tools and selected 200patients with mental and behavioral disorders due to use of alcohol among the alcohol users. Second,withdrawal scale determined among the selected patients using by SAWS test. In third stage, resultsof psychotherapy among the patients with mental and behavioral disorders due to use of alcohol hadbeing compared with results of control group without psychotherapy. In statistical analysis, there isused CI at P<0.05andRR=p1/p2) at the CDC-EpiInfoOE2.3.RESULTS: Result of psychotherapy among patients in trial group with mental and behavioral disordersdue to use of alcohol was identified directly 80.1%, and after 3 months - 82.3% on SAWS test,respectively direct result was 64.20% (RR=1.2) and after 3 months - 47% (RR=1.8)among patientsof control group on SAWS test .Furthermore, results were 77.3%, and after 3 months - 75.3% on SADD test among trial grouppatients. Same time the direct result was 71.30% (RR=1.1), and after 3 months -66.50% (RR=1.1)among patients in control group on SADD test.

Background. Most people infected with Cytomegalovirus (CMV) have no signs or symptoms, but newborns andpeople with weakened immune systems are more at risk of developing signs and symptoms. CMV remains in thebody throughout a lifetime. Infected people may spread infection.Goal. The main aim of this study is to determine the prevalence of infection of cytomegalovirus (CMV) by enzymeimmunosorbent assay.Materials and Methods. In this study included subjects, age range of 17-60 years (total of 716) blood donorswho were served by the National center for transfusion medicine. Screening for Cytomegalovirus CMV IgG andIgM specific antibody concentrations determined using DIALAB ELIZA kits.Results. CMV-specific IgG antibodies were detected in 99.2% (710) of the studied subjects and, specific IgMantibodies in 0.1% (1). IgG antibodies positive individuals are compared to 4 blood groups and (p = 0.01) differencewas not statistically significant. IgG antibodies negative 6 individuals are included all in blood type B(III). IgMspecific antibodies positive (1) individual was in the age group 26-40 years, female and type of regular donor.Conclusion. We can conclude from our results of the determination of CMV IgG and IgM specific antibodiesshows that a number of risks of transfusion transmitted infection cytomegalovirus.

IntroductionIn the total increase in the rate of smoking in the population of Mongolia, the smoking rate among women of the age of 15-24 years old increased up to 4.2% which is higher by 1.9 points against the level of the year 2005. The average age of starting smoking among women decreased by 3 years compared to 2005 evidencing the increase in the prevalence of smoking among the female population. However, there is no study on prevalence of smoking focusing on school age girls. Researches on the prevalence of smoking and KAP of the population shall play an important part in reduction of tobacco consumption, assisting smoking cessation and determining the effective forms of anti-tobacco communications.Materials and MethodsThe study of the tobacco consumption and risk factors among the school age girls of the 6-11th grades is a cross-sectional study covering the school age girls of 6-11th grades of Suhbaatar, Chingeltei, Bayangol and Songinokhairkhan districts of Ulaanbaatar and Uvurkhangai, Khovd, Dornod and Umnugobi aimags. The participants of the study were selected by random sampling. The study used qualitative and quantitative methods. The study involved 1208 school age girls of 6-11th grades. The data of the survey was analyzed with SPSS software.ResultsEvery third or 33.7% of the schoolchildren of 6-11th grades smoke. The rate of smoking is as twice higher in the metropolitan settings as in provincial aimags. The answer that there are smokers in the family given by 64% of the respondents implies that two in three target age school girls are exposed to second-hand smoking. Every fifth of the girls find themselves in tobacco affected areas daily. Of the target age/gender group, 36.5% reported that they buy cigarettes in kiosks, shops and stores and one in each two schools have any form of tobacco selling points within 100 meters which implies a potential impact of the surrounding environment. Around 90% of the participants of the quantitative survey were able to correctly list the health hazards of smoking, however, the qualitative survey results demonstrate that they have confusions on the health hazards of smoking and have insufficient understanding of the long term effects of smoking.Conclusions:1. Girl in the metropolitan settings smoke more than in provincial settings.2. Smoking at schools and public places are common in both metropolitan and provincial areas.3. The smoking behaviors of the girls are influenced by their and peers. insufficient knowledge 4. It was identified by the research that all smoking girls in the capital and in provinces are wishing to quit smoking.5. Though the girls receive information on tobacco from mass media, friends and Health Education subject, the information received through these channels are insufficient to impact their knowledge and change their behavior.6. The number of school children exposed to the impact of tobacco tends to increase by their age.

Background: Establishment of quantitative reference intervals of white blood cells and its subpopulations using a high accuracy analytic system is essential for clinical medicine, public health, and anthropology. We are unable to identify peer-reviewed literature sources describing white blood cell counts and their subpopulations using monoclonal antibodies to specific surface antigens in healthy Mongolians. This study aimed to measure the counts of white blood cells and their subpopulations in healthy Mongolians using flowcytometry. Materials and Methods: The absolute number (cell/L) of leukocytes (CD45+), granulocytes, monocytes and lymphocytes were measured by Magnetic Activated Cell Sorting Assay (MACSQuant Analyzer 10) in 287 blood donors (158 males and 129 females) 17-64 years of age (mean age 33.1±12.4). Peripheral blood samples were collected at the time of blood donation at the National Center for Transfusion Medicine. Results The mean values of leukocytes and granulocytes were lower in donors over 30 years of age (ANOVA: F=4.408, p=0.002 and F=5.685, p=0.001) and regression analysis demonstrated indirect correlation between counts of these cells and age of donors (r= - 0.198, p=0.001 and r=-0.221, p=0.001, respectively). Gender-related differences in white blood cell counts were not found.
Mean value of lymphocyte count in donors investigated in spring (May and March, n = 87; 2224.6±775.3) was significantly higher than those in winter (December – February, n=180; 1613.2±454.3, p=0.001) and autumn (October, n=20; 1576.1±438.6, p= 0.001).
Comparing of our findings with the data from available literature shown that healthy Mongolians have lower leukocyte count compared with Koreans, Chinese Han population and lower mean value of lymphocyte count comparing with Korean, Chinese Han population, and Arabian (Saudi Arabia) populations.