Background: Development and progression of cancer is accompanied by different morphological and functional changes of cells. One of the most important changes is the expression and activity of enzymes in the cellular fatty acids metabolism that reflects in cell membrane lipid composition and increases fluidity of cancer cell membrane. The Ellipin, prepared from bovine liver, is a newly developed anticancer agent containing several important fatty acids.Goal: To investigate effects of Ellipin on hepatic cancer cell function such as proliferation, migration and adherent activity and apoptosis of cancer cell lines in vitro.Materials and Methods: This study was conducted in the Laboratory of Molecular Biology, Institute of Biology, MAS. The Ellipin was developed in the Drug Research Institute of Monos group. HepG2, HCC, 23132/87, MDCK cells lines and the primary liver cancer cells (PCC) were used for proliferation assay. Only HepG2 cell line was used for MTT, Migration, Spreading and Apoptosis assays.Results: The results of proliferation assay showed that the ellipin decreased the proliferation activity of HepG2 and PCC cells depending on concentrations; in 50μg/ml 2-3 times, 250μg/ml fully stopped cells divisions. The Ellipin reduced mitochondrial reeducates enzyme activity of HepG2 cells depending on its concentrations. For example, in 50μg/ml ellipin concentration case, the number of alive cells decreased 2 times. The migration of HepG2 cells treated with 100μg/ml ellipin was decreased by 22.3% compared to the control cells. Also the number of adhered cells was reduced by Ellipin treatment. After 50μg/ml, 100μg/ml, 250μg/ml ellipin treatment, the number of apoptic cells were 14,6%, 45,6%, 100% of initial culture cells, respectively.Conclusions: Our results showed that the Ellipin suppresses HepG2 cancer cell proliferation and decreases migration and spreading activities and also inducts the cell apoptosis.

Hysterectomy is still the most common major operation performed by gynecologists. Having said that the incidence of hysterectomy has increased last 20 years with new minimally invasive technique. Laparoscopy assisted vaginal hysterectomy has advantage like small incision, less operative pain, quicker recovery, less hospital stay comparing to the abdominal open surgery. Objective: To compare the outcome of abdominal hysterectomy and laparoscopic vaginal hysterectomy.The study population comprised all patients who had LAVH and abdominal hysterectomy at First Maternity Hospital, from June 2013 to December 2014.The mean age of both group is same (46.2±5.3). Duration time of LAVH is approximately 2.30±-5.9minutes and of total abdominal hysterectomy is a 1.41±2.7 minute. The general blood loss during LAVH is 74.4±19 ml and during total abdominal hysterectomy is 185.2 ± 67 ml. After LAVH any patient had no complaints. After LAVH the patient stayed at the hospital for 3 days. LAVH took significantly longer operating time than TAH. Blood loss was jess during LAVH. Hospital stay after LAVH was less than TAH. Drug cost is more case LAVH.