Dysfunction of microRNA (miRNA) is associated with occurrence and development of tumor.MiRNA are very stable in blood serum,significantly tumor-related and tissue-specific.The detection of serum miRNA is convenient,little-invasive and fast,and then serum miRNA can be used as a new biomarker for tumor diagnosis.

The G protein-coupled receptor family C,member 5,group A (GPRC5A) gene is known as retinoic acid-induced gene,which is mainly distributed in lung tissue.The expression of GPRC5A in lung cancer is significantly decreased compared with normal lung.GPRC5A leads to lung cancer through knockout mice,which is proven to be a suppressor gene of lung cancer.GPRC5A may be a novel biomarker for the diagnosis of lung cancer and a new target for the treatment of lung cancer.

Objective To evaluate the clinical features and various diagnostic methods for sarcoidosis.Methods The data of 28 cases with sarcoidosis confirmed by pathology were reviewed and analized,and different diagnostic methods were compared.The disease was evaluated by regular serological tests,chest X ray film,chest high resolution computed topography(HRCT),bronchofibroscopy,and pulmonary function test.Results These patients shared a typical clinical presentation,consisting of dyspnea,cough,subskin nodes,enlargement of peripheral lymphnodes and splenomegaly.Laboratory test revealed elevated serum blood sedimentation,alkali phosphatase,Angiotensin-converting,and urine calcium.The saricoidosis was confirmed by pathological biopsy for bronchial mucosa,lung tissues,peripheral lymphnodes,subskin nodes biopsies and spleen.HRCT showed its superiority to traditional X ray film examination in fingding early staging chest sarcoidosis.Conclusions Chest HRCT and bronchopulmonary biopsy through bronchofibroscopy possess significant clinical value for sarcoidosis diagnosing and staging.And extropulmonary lessions biopsy also plays a complementary role to some extent.

OBJECTIVETo discuss the application and clinical effect of damage control concept in the treatment of severe limbs fractures combining with multiple trauma.

METHODSFrom July 2009 to July 2012, 30 patients with severe limbs fractures combining with multiple trauma were treated with the damage control concept, included 20 males and 10 females with an average age of (34.03 ± 12.81) years old ranging from 20 to 60 years old; the ISS averaged (35.00 ± 12.81) points (ranged from 26 to 54 points). And the control group also contained 30 patients with severe limbs fractures combining with multiple trauma treated by the traditional operation from June 2006 to June 2009, there were 23 males and 7 females with an average age of (34.23 ± 11.04) years old ranging from 18 to 65 years old. The ISS averaged (35.56 ± 11.04) points (ranged from 26 to 51 points). The age, gender, ISS, Gustilo classification, operation time, intraoperative blood loss, blood transfusion,postoperative complications and mortality rate were observed and compared.

RESULTSIn the damage control concept group,there were 28 cases surviving and 2 cases (6.7%) death; 6 cases of postoperative complication included 2 cases of adult respiratory distress syndrome, 1 case of multiple organ failure, 1 case of disseminated intravascular coagulation and 2 cases of wound infection. In the control group, there were 22 cases surviving and 8 cases death(26.7%); 13 cases of postoperative complication included 4 cases of adult respiratory distress syndrome,2 cases of multiple organ failure, 2 cases of disseminated intravascular coagulation and 3 cases of wound infection. There were no statistically significant differences between two groups in age, gender, ISS, Gustilo classfication and complication (P > 0.05), however there were statistically significant differences in mortality rate, operation time, blodd loss, blodd transfusion between two groups (P < 0.05).

CONCLUSIONDamage control concept is used to treat severe limbs fractures combining with multiple trauma which has the rapid and effective therapy, can improve survival rate and reduce complication.

Adolescent ; Adult ; Aged ; Embolism, Fat ; prevention & control ; Extremities ; injuries ; Female ; Humans ; Male ; Middle Aged ; Multiple Trauma ; surgery ; Postoperative Complications ; prevention & control

Objective To investigate the features of MR imaging of acute high voltage electric injury in forearm muscle. Methods Nine patients (17 forearms, 8 males and 1 female, 15～36 years of age) with clinically and pathological proved acute high voltage electric injury were studied on MRI retrospectively. MRI studies were obtained within 72 hours on Siemens 1 0 T MR scanner. 2 forearms were examined with body coil, and 15 with head coil. The severe area was placed as near as possible to the isocenter in the magnet and was used as the center of the MR imaging acquisition. Spin echo T 1 weighted images, spin echo and fast spin echo T 2 weighted images were acquired in all patients. 14 out of 17 were performed with Ⅳ administration of Gd DTPA. Results All 17 forearms had fascistomy after MRI. 11 had only debridement. The lesions were mainly observed in the flexor digitorum supericialis or profunduds muscle appearing as isointense on T 1 weighted images, hyperintense on T 2 weighted images, and strongly enhanced after Ⅳ administration of Gd DTPA in 8. The proximal aspect of the lesion appeared as sharp knife in 11. There was a weaker twitch response to electrocauterization in the injury muscle than in healthy muscle. It was variably necrotic in histopathology. Two transitional zones accompanied with the suffered forearm in 2, and one transitional zone in 6. Both of them had well defined margin. 6 forearms had amputation after debriding. There was Ⅰ,Ⅱ,and Ⅲ mixture signal all over the forearms. The proximal lesions showed type Ⅰ changes. Distal to the zone of forearm showed type Ⅱ and Ⅲ pattern appearing as isointense on T 1 weighted images, hyperintense and hypointense on T 2 weighted images. It was hardly enhanced after Ⅳ administration of Gd DTPA. There was no twitch response to electrocauterization in the injury muscle. It was almost completely necrotic in histopathology. ALL amputated forearms had two transitional zones and ill defined margin. The second transitional zone was enhanced something like flower border. Conclusion MR imaging of acute high voltage electric injury in forearm appeared as three kinds of signal mode, which was closely related with histopathology. MRI was useful in dealing with clinic problem and in judging the prognosis.

Dendritic Cell Sarcoma, Interdigitating ; Humans ; Lymph Nodes ; Male ; Middle Aged

Objective To investigate the effect of catalpol from Radix Rehmanniae on A?25-35-induced apoptosis of PC12 cells.Methods PC12 cells were routinely cultivated and treated by A?25-35(final concentration,20 ?mol/L) 24 hours after the addition of catalpol or saline.Forty-eight hours later,cells were examined for viability and apoptosis by MTT method and TUNEL method,respectively,while Bax and Bcl-2 mRNA expression were analyzed by semi-quantitive RT-PCR. Results Catalpol could significantly elevate the viability at 1?10-5 mol/L and 1?10-4 mol/L(P

Adolescent ; Fatal Outcome ; Female ; Fibromatosis, Aggressive ; diagnostic imaging ; surgery ; Humans ; Radiography

A rare strain of actinomycetes, with broad-spectrum antimicrobial activity, was isolated from the soil samples from the farmland in the area of Yaohu lake in Nanchang. The information about the taxonomic identification, such as the morphology, physiological properties, cell components and 16S rRNA gene se-quences, suggested that the rare strain of actinomycetes was identified as Micromonospora carbonacea.

Based on the strain of Micromonospora carbonacea JXNU-1 with board-spectrum antimicrobial activity, the technology for the isolation and purification of antibiotic from the fermentation broth of the Micromonospora carbonacea, and its physical-chemical properties were studied. The results showed that, the antibiotic was stable under the condition of high temperature and alkali, but not in acid solution. After the pretreatment of centrifugation and filtration to remove the cells and lipids, the antibiotic was absorbed to negative exchange resin, and the impurity was excluded when 2 mol/L NaCl was used as primary eluent. The antibiotic could be eluted with 20% alcohol as eluent, and the eluting speed of the antibiotic was greatly accelerated as 2 mol/L NaCl was added into 20% alcohol as final eluent. Aqueous solution of the antibiotic was yielded from the alcohol-salt eluant by decompression concentration to wipe off alcohol and by dialysis to exclude salt. One active component was detected in antibiotic solution by paper chromatography, and theHPLC purity was over 99%. As the antibiotic shows positive color-forming reaction to Molish reagents, Benedict’s reagents and Diohenvlamine reagents, combined with the characteristics of absorption spectra, it is deduced that the antibiotic belongs to nucleoside antibiotics.