Tissue array is a technique by taking and transfering pieces of tissue from different block paraffin to new block. After cutting the authors get a slice with a lot of different samples. This technique saves time, chemistry and work. Using this technique to study 220 Gastric cancer samples. The expression of protein P53 in Gastric cancer tissue is 52.3%. In situ hybridization was used to find the EBERs (EBV Encoded small RNAs) of EBV in Gastric cancer tissue
Tissue Array Analysis ; Epstein-Barr Virus Infections ; Stomach Neoplasms

No abstract available.
Epstein-Barr Virus Infections* ; Herpesvirus 4, Human* ; Humans ; Lymphoma*

Epstein-Barr Virus Infections ; Humans ; Lymphoproliferative Disorders ; diagnosis ; genetics

Epstein-Barr virus (EBV), a double-stranded DNA virus with an envelope, is a ubiquitous pathogen that is prevalent in humans, although most people who contract it do not develop symptoms (Kerr, 2019). While the primary cells EBV attacks are epithelial cells and B lymphocytes, its target range expands to a variety of cell types in immunodeficient hosts. Serological change occurs in 90% of infected patients. Therefore, immunoglobulin M (IgM) and IgG, serologically reactive to viral capsid antigens, are reliable biomarkers for the detection of acute and chronic EBV infections (Cohen, 2000). Symptoms of EBV infection vary according to age and immune status. Young patients with primary infection may present with infectious mononucleosis; there is a typical triad of symptoms including fever, angina, and lymphadenectasis (Houen and Trier, 2021). In immunocompromised patients, response after EBV infection may be atypical, with unexplained fever. The nucleic acid of EBV can be detected to confirm whether high-risk patients are infected (Smets et al., 2000). EBV is also associated with the occurrence of certain tumors (such as lymphoma and nasopharyngeal carcinoma) because it transforms host cells (Shannon-Lowe et al., 2017; Tsao et al., 2017).
Humans ; Trachea ; Epstein-Barr Virus Infections ; Herpesvirus 4, Human ; Virus Diseases ; Fever ; Granuloma

Epstein-Barr virus (EBV) infection occurs by distinct mechanisms across different cell types. EBV infection of B cells in vitro minimally requires 5 viral glycoproteins and 2 cellular proteins. By contrast, infection of epithelial cells requires a minimum of 3 viral glycoproteins, which are capable of interacting with one or more of 3 different cellular proteins. The full complement of proteins involved in entry into all cell types capable of being infected in vivo is unknown. This review discusses the events that occur when the virus is delivered into the cytoplasm of a cell, the players known to be involved in these events, and the ways in which these players are thought to function.
B-Lymphocytes ; Epithelial Cells ; Epstein-Barr Virus Infections ; Herpesvirus 4, Human ; Viral Proteins ; Virus Physiological Phenomena

OBJECTIVETo analyze the characteristic of nuclear antigen 1 gene and latent membrane protein 1 gene of Epstein-Barr virus in primary EBV infection in children in Beijing area in 2005-2012.

METHODSPolymerase chain reaction (PCR) was used to amplify the EBNA-3C, EBNA1 and LMP1 genes. The amplified products were sequenced directly and the sequences were analyzed by BioEdit 7. 0. 9 and MEGA 4. 0. 2.

RESULTSType A EBV was detected in 98% samples. Nucleotide sequence analysis of the carboxy-terminal region of EBNA1 showed that Vvvl was deteted in 98% samples. DNA sequence analysis of LMP1 C-terminus indicated that China 1 was 90% in this study. There were no significant differences in the frequency of Vvv1 and China 1 between the IM and HLH samples (P = 1.00). Linkage analysis of EBV types, EBNA1 and LMP1 variants indicated that 90% of EBV type A was associated with EBNA1-Vvv1 variant and LMP1-China 1 variant in 40 cases. Full length of LMP1 gene was successfully amplified in 35 cases. Four Chinese groups (CG1-4) were identified. The percentage of CG1-CG4 were 85%, 6%, 6% and 3%, respectively.

CONCLUSIONEBV type A is predominant in primary EBV infection in children in Beijing Area. EBNA1-Vvv1 and LMP1-China 1 variants were predominant genotypes in this area. There is a high linkage between EBNA1-Vvv1 variant and LMP1-China 1 variant. Four Chinese groups (CG1-4) were identified according to the full length of LMP1 gene and CG1 was the most prevalent.

China ; Epstein-Barr Virus Infections ; virology ; Epstein-Barr Virus Nuclear Antigens ; genetics ; Genetic Linkage ; Herpesvirus 4, Human ; classification ; genetics ; Humans ; Time Factors ; Viral Matrix Proteins ; genetics

No abstract available.
Epstein-Barr Virus Infections/*complications ; Herpesvirus 4, Human/*physiology ; Humans ; Neoplasms/*etiology

EBV infection has been causally associated with incidence of many carcinomas. EBV-associated gastric carcinoma (EBVaGC) has been classified as a unique gastric carcinoma subset, suggesting EBV infection is related to the development of gastric cancer. In this study, general trends of EBVaGC studies for last half-decades were reviewed in several perspectives of clinical significance, virological importance and etiological interests. Throughout this comprehensive reviewing, new study trends of EBV and EBVaGC for next half-decades were suggested.
Epstein-Barr Virus Infections ; Herpesvirus 4, Human* ; Incidence ; Methylation ; Prognosis ; Stomach Neoplasms

Hydroa vacciniforme (HV) is a rare self-limiting disease characterized by vesicles and crust formation after sun exposure. However, cases with HV-like eruptions and severe systemic symptoms have also been described. It had been regarded as a different entity from typical HV. But latent Epstein-Barr Virus (EBV) infection has also been detected in typical HV patients and the possibility has been suggested that typical HV and atypical HV are variants within the same disease spectrum of EBV-associated lymphoproliferative disorders, which ranges from typical HV as a benign pole to severe HV progressing to hematologic malignancy as a malignant pole. Chronic active Epstein-Barr virus infection (CAEBV) is an atypical illness associated with serologic evidence of persistent EBV infection. The extremely high levels of EBV measured in affected tissue or peripheral blood are the most recently proposed diagnostic criteria. Recently, we found high levels of EBV in the peripheral blood of patients with EBV-associated HV-like eruptions.
Epstein-Barr Virus Infections ; Hematologic Neoplasms ; Herpesvirus 4, Human ; Humans ; Hydroa Vacciniforme ; Lymphoproliferative Disorders ; Solar System

No abstract available.
Epstein-Barr Virus Infections ; Guillain-Barre Syndrome* ; Herpesvirus 4, Human* ; Humans