Objective To study the expression of cyclooxygenase 2 (COX-2) in neonatal rats after hypoxic-ischemic brain damage (HIBD). Methods A model of HIBD in the left brain of 7-day-old Wistar rats was established. The ischemic cortex was sampled for analysis at 2, 6, 24, and 72 h, and 7 d after the onset of hypoxic-ischemic damage. The expression and number of the positive cells of COX-2 in the cortex and hippocampus at different time points were observed by immunohistochemistry. Results Low level of COX-2 immunohistochemical expression was observed in the control group. COX-2 positive cells were upregulated in ischemic brain at 2 h and peaked between 6-24 h significantly (P

Objective To study the cell apoptosis after injecting NS398 in neonatal rat brain injured by hypoxia and ischemia.Methods The model of hypoxic-ischemic brain damage(HIBD)was established in 48 Wistar rats.Intraperitoneal injection of NS398 at dose of 5,20,40 mg/kg respectively was carried out in 36 HIBD rats 30 min before hypoxia.There were also sham operation group in which the normal saline took the place of NS398.Ischemic cortex and hippocampus were sampled for analysis at 24 h,and 7 d after the onset of hypoxic-ischemic damage.The expression and number of apoptotic cells in the cortex and hippocampus were examined with TUNEL.Results The percentage of TUNEL-positive cells in HIBD group was higher than that of sham operation group.The percentage of TUNEL-positive cells in NS398 groups significantly decreased as compared with that of HIBD group.NS398 group at dose of 20 mg/kg was of no difference with NS398 group at dose of 40 mg/kg,but significantly lower than NS398 group at dose 5 mg/kg.Conclusion NS398 can reduce the TUNEL-positive cells,and 20 mg/kg and 40 mg/kg are the more effective dosage.NS398 is of the effective capability to inhibit the brain damage in the growth period when HI happened.

Objective To observe the effect of β-blocker on dilated cardiomyopathy (DCM) in children.Methods Sixty-one children cases of DCM in this hospital from 1995 to 2015 were retrospectively analyzed and divided into the treatment group(taking β-blocker,n=35) and control group(non-taking β-blocker,n=26) according to whether taking β-blocker.The clinical effect of β-blocker was preliminarily observed.Results The heart rate,LVDD,LVSD and LA after treatment in the treatment group were significantly decreased compared with before treatment(P＜0.05),while EF was significantly increased(P＜0.05);LVDD after treatment in the control group was significantly decreased(P＜0.05),while hear rate,RV,LVDD,LVSD,LA,MV and EF had no obvious change compared with before admission(P＞0.05).The heart rate,LVDD,LVSD and EF after treatment had statistical difference between the treatment group and control group (P＜0.05),the heart function after treatment in the treatment group was significantly improved compared with the control group (P＜0.05).The heart function improvement degree after treatment in the patients with hypertension of the treatment group was significantly better than those with normal blood pressure(P＜0.05).DCM with hypertension was significantly improved after treatment and was better than DCM without hypertension (P＜0.05).The The heart function improvement degree after treatment in the patients with carvedilol treatment was obviously better than that in the patients with metoprolol treatment(P＜0.05).Conclusion β-blocker could be used in the treatment of DCM,its effect is especially good in DCM children patients with hypertension,in which the effect of carvedilol is better than that of metoprolol.

OBJECTIVETo observe the ability of triple helix-forming oligonucleotides (TFOs) modified with manganese porphyrin to combine with and cleave HBV DNA fractions.

METHODSTFO were modified with manganese porphyrin and acridines, and then reacted with the (32)P labeled HBV DNA fragments at 37 degrees C in vitro (pH 7.4). Electrophoretic mobility shift assays and DNase I footprinting tests were used to show the affinity and specificity of TFO to bind to target sequences. The ability of TFO to cleave HBV DNA fragments was tested by cleavage experiments.

RESULTSTFO modified with manganese porphyrin and acridine could bind to the target sequence in a sequence-dependent manner, with a Kd value of 3.5 x 10(-7) mol/L and a relative affinity of 0.008. In the presence of potassium monopersulfate (KHSO(5)), TFO modified with manganese porphyrin and acridine could cleave the target sequence where the triplex DNA was formed.

CONCLUSIONIn the presence of KHSO(5), TFO modified with manganese porphyrin and acridine could bind and cleave the target HBV-DNA in a sequence-dependent manner.

DNA ; drug effects ; pharmacology ; DNA, Viral ; chemistry ; drug effects ; Hepatitis B virus ; genetics ; Manganese ; pharmacology ; Metalloporphyrins ; pharmacology ; Potassium Compounds ; pharmacology ; Sulfates ; pharmacology