The objective of this study was to examine the association between body mass index (BMI) and blood pressure. Two sets of cross-sectional data were obtained from annual health examinations for adults aged 40 years and over (n=1,327 in 1993; n=1,302 in 2000) in Tsunagi area of Kumamoto Prefecture, Japan. BMI was associated with mean blood pressure and with prevalence of hypertension both in 1993 and 2000. The association was independent of age, smoking status and alcohol consumption. A significant increase in risk of hypertension was found in most categories of BMI 25.0 and above, and a greater than three fold increase in those with BMI of 27 and above compared with those with BMI of 18.5-22.9. Although mean blood pressure and prevalence of hypertension sharply decreased in 2000 compared with that in 1993, BMI was positively and independently associated with increased blood pressure.

OBJECTIVEClarify the relation between the complaints of wheelchair users and the vibration characteristics of the wheelchair, to improve wheelchair comfort and design.

METHODSThe question naires were distributed to 33 wheelchair users directly by the experimenter in order to identify the causes of complaints from wheelchair vibrations that they experienced. The vibration transmissibility of wheelchairs of ten subjects was measured to clarify the causes of complaints of wheelchair vibration according to the ISO 10326-1 standard in the laboratory using a broadband random vibration spectrum with a frequency-weighted vibration magnitude of 0.1 ms(-2) r.m.s. over the frequency range from 0.2 to 100 Hz. Each vibration exposure lasted 60 seconds.

RESULTSThe following findings were clear from the questionnaire results; (i) the vibration rom the wheelchair affected psychological comfort; (ii) the effects of different riding surfaces were important engineering issues affecting wheelchair ride comfort; (iii) the wheelchair users felt the vibration during wheelchair usage at locations on the neck, lower back and buttocks; (iv) vertical vibration was the most noticeable vibration from the wheelchair to each participant's body. The following findings were clear from the results of the transmissibility measurement of the wheelchair; (i) the resonance frequency-ranges of the transmissibility of the wheelchair showed significant differences between the subjects; (ii) intra-subject variability from three repeated transmissibility measurements was small; (iii) the first resonant frequency occurred approximately 5 to 7 Hz and the second resonant frequency occurred at around 8 Hz and the third resonant frequency occurred approximately 13 to 15 Hz; (iv) the magnitude of the peak transmissibility varied from 1.3 to 2.6.

CONCLUSIONFrom the comparison of the results of questionnaires and the transmissibility measurement of the wheelchair, the resonance frequency-ranges of the maximum vibration transmissibility of the manual wheelchairs were consistent with the frequency-ranges of the body parts of the causes of the complaints of wheelchair users. In addition, from these experimental results, it was suggested that the main point for improving a wheelchair user's comfort was to reduce the wheelchair seat vibration transmissibility at around 8 Hz and also to design wheelchair stiffness and damping characteristics to minimize vibration transmission at specific frequencies at body locations that caused the discomfort reported by wheelchair users.

OBJECTIVEThe aim of this study was to explore the association between exposure to specific pesticides (including endocrine disrupters) of greenhouse workers and spontaneous abortion in their spouses.

METHODSA group of exposed workers was compared with a non-exposed group. The risk of spontaneous abortion was evaluated using a logistic regression model.

RESULTSA significantly higher rate of spontaneous abortion was observed among spouses of workers exposed to specific compounds (atrazine, benomyl-carbendazim, carbaryl and DDT) in comparison with spouses of the unexposed group. The logistic regression model confirmed the increased risk of spontaneous abortion (OR=11.8; 95% CI 2.3-59.6).

CONCLUSIONSThese findings suggest that occupational exposure to pesticides might have an adverse effect on the partner's reproductive health.

OBJECTIVESTo clarify whether di (2-ethylhexyl) phthalate (DEHP) has immunotoxic effects on both the expression of surface molecules (CD3, CD4, CD8 and CD28) on T cells of the thymus and spleen in male C57BL/6 mice.

METHODSAnimals were orally administered a 0.1% or 0.2% DEHP-containing diet for 10 or 20 days. Dietary corn oil was used as the vehicle for DEHP in preparing the diet.

RESULTSSignificant hepatic hypertrophy was clearly observed in the DEHP-exposed groups, while no atrophy was seen in the thymus or spleen in any treatment groups. In the thymus and spleen, no variation in the proportions of both T cells expressing CD3, CD4 and CD8 was shown with cytometry analysis. The surface expression of CD3, CD4, CD8 and CD28 on both T cells was also invariable in all analyzed stages of thymic differentiation and in the spleen. No effect of DEHP on mitogenesis was shown in the splenic T cells with anin vitro [(3)H]-thymidine-incorporation technique.

CONCLUSIONSDEHP is probably not an immunosuppressor, particularly for T cells.

OBJECTThe purposes of this study were to investigate the actual status in subjective symptoms of fatigue (SSF) and lifestyles of young male students living in two different life spheres of the Tokyo metropolitan area and a rural area, and to find, regional differences in these characteristics and relations between SSF and lifestyles in school life.

METHODSA questionnaire regarding SSF and lifestyle was administered to 412 healthy male students (16-18 yr) attending the two National Colleges of Technology located in the Tokyo metropolitan area (MA) and a local city (LC). Statistical techniques such as a chi-square test and Hayashi's Quantification Theory I analysis were used to examine relations between SSF and lifestyles.

RESULTS AND CONCLUSIONA significant difference was found only in "feeling of physical disintegration,", and the MA group was higher than the LC group. The MA students showed shorter sleeping hours and earlier awaken time compared with LC students. In the relations between SSF and lifestyles, sleeping habit was an important factor significantly relating to SSF in both groups. Paying attention to good sleeping habits is an important countermeasure for preventing SSF in the male students.

OBJECTIVESThe epigallocatechin-3-gallate (EGCg) that is present in human diet originates mainly from tea leaves. Catechins have a number of possible application as medicines, however, there is no consistent evidence showing their influence on the gastrointestinal tract. Thus, the aim of the present study was to investigate the effect of EGCg on the motility of the murine isolated intestine.

METHODSSegments of jejunum submerged in Krebs buffer were exposed to EGCg and the response was recorded under isometric conditions.

RESULTSEGCg induced a dose-dependent inhibition of spontaneous activity in the jejunum. EGCg induced a decrease in the amplitude and frequency of jejunal contractions. moreover, the rythmicity of spontaneous, activity was altered in the presence of EGCg. A significant effect of EGCg was observed in the presence of 10(-4) M. The effect of EGCg was in part inhibited by pretreatment with methylene blue (guanylate cyclase inhibitor), while tetrodotoxin, (sodium channel blocker), L-nitro arginine methyl ester (nitric oxide synthase inhibitor), and N-ethylmaleimide (adenylate cyclase inhibitor) showed no effect.

CONCLUSIONSThe results of the present study suggest that EGCg inhibits the motility of the jejunum by direct action on smooth muscle cells where a guanylate cyclase-dependent mechanism may be partly involved.

OBJECTIVESThe purpose of this study was to quantitatively and qualitatively examine a gender difference of subjective symptoms of fatigue (SSF) in healthy students.

METHODSThe subjective fatigue scale for young adults (SFS-Y) consisted of the following 6 subscales: concentration thinking difficulty, languor, reduced activation, reduced motivation, drowsiness and feeling of physical disintegration. The SFS-Y was administered to 5,435 students aged 15 to 20 yr. Student's t test was used to examine quantitative gender differences for items and factor scores. The qualitative gender difference was examined by comparing factor structures obtained from confirmatory factor analysis. A similarity in the factor structure was evaluated from: 1) interpreted factor names, 2) factor loadings, 3) degree of fit, and 4) congruence coefficient.

RESULTSA complaint of SSF shows gender differences, but the difference is not remarkable. The factor structure of SSF in males and females is suggested to be the same, because six factors with the same name were interpreted in both groups and the similarity of factor structure was very high.

CONCLUSIONThere are some qualitative gender differences in SFS, but no qualitative gender differences in factor structure.

Catalase-deficient mouse strains was initially established by Feinstein et al. through a large scale screening of the progeny of irradiated C3H mice in 1966. Later, Feinstein provided the mice of catalase mutant strain C3H/AnICs(a)Cs(a) (wild-type), C3H/AnICs(b)Cs(b) and C3H/AnlCs(c)Cs(c) to Okayama University Medical School in Japan. It is known that a point mutation at amino acid 11 (from glutamine to histidine) of acatalasemic mouse catalase and a point mutation at amino acid 439 (from as paragine to serine) of hypocatalasemic mouse catalase are responsible for the catalase deficiency of acatalasemic and hypocatalasemic mice, respectively. Recently, a liver cell line from an acatalasemic mouse andEscherichia coli (E. coli) strains with murine normal, hypocatalasemic, or acatalasemic catalase have been established. The construction of these new systems would be useful for studying the effects of oxidative stress at the cellular level. In this review, we give a brief overview of recent findings of studies in utilizing the catalase-deficient mice and evaluate the possibility of these mouse strains as a candidate animal model for oxidative stress research.

OBJECTIVESTo use the evaluation of a specific brain function obtained by optical topography. This system uses a non invasive method to measure brain function unlike other major systems.

METHODSTwelve optical fibers were attached to the subject's head. Hemodynamic changes in the motor cortex were measured during finger tapping before and after alcohol intake for eachALDH2 genotype.

RESULTSDifferent hemodynamic changes in the motor cortex were observed among, theALDH2 genotypes.

CONCLUSIONSOptical topography is a useful tool for evaluating specific brain functions. Further research is needed on the relations between various environmental factors and brain functions by optical topography.