Objective To elucidate the neurological m ec hanism of the referred pains in pelvic floor from prostate and the relationship between pain from prostate and pelvic floor muscle dysfunction. Meth ods The prostate was electrically stimulated,and the elicited action potentials were recorded from iliohypogastric,ilioinguinal,genitofemoral,latera l femoral cutaneous,femoral,sciatic nerves,and compound muscle action potentials (CMAP) were recorded from anal external sphincter. Results Action potentials were elicited principally in the genitofemoral nerve (93 .3%),ilioinguinal nerve (55.5%);the action potentials of the genitofemoral nerve were not influenced by transaction of the cervical spinal cord,whereas they dis appeared immediately after rat death; the action potentials were reduced conside rably after destruction of the lumbosacral sympathetic trunks.CMAPs were elicite d in the anal external sphincter in 93.3% of the rats (14/15). Conc lusions Electrical stimulation of the prostate induced reflex discha rges in the nerves to perineal,pelvic floor areas.It was postulated that the ref lex discharges are related to muscle contraction leading to referred pain in the perineal,pelvic floor areas.Pain from prostate is correlated significantly with pelvic floor muscle dysfunction.

0.05 ).Fifty five patients of HoLEP group (78.6%,55/70) and 58 patients of TURP group(82.9%,58/70) were followed up at the third and sixth month postoperatively.In TURP group,6.9% of the patients(4/58) needed blood transfusion and 3.4%(2/58)developed TURS.In HoLEP group, no one needed blood transfusion or developed TURS. The differences were significant between the 2 groups ( P

Objective To study the neurogenic mechanism of urinary bladder dysfunction changes in the aged rats. Methods Wistar rats were divided into aged group (24 month old) and young adult group (6 month old, served as the control group). The reactivity of urinary detrusor strips in the two groups to different concentrations of carbachol, noradrenalin, isoproterenol, adenosine triphosphate (ATP) and atropin, was measured respectively using an in vitro tissue bath technique. Results Compared with the young adult rats, significantly increased relaxation response to high concentration of isoproterenol and contractile response to ATP were found in aged rats. No difference in responses to carbachol, atropin and noradrenalin was found in the aged rats. Conclusion The dysfunction in aging bladder may be related to the purinergic and ? adrenergic response modifications.

Objective To establish a brand new model of bladder outflow obstruction (BOO) at single cell level in vitro to offer a more stable and scientific experimental base. Methods Recurrent mechanical stretch generated by vacuum facility was applied to cells attached to flexible membrane of special culture plate, which led to 10%, 20% and 30% elongation of them. Immunocytochemistry was used to analyze the expression of ?-actin (sign of contractile phenotype) and RT-PCR was performed to detect the dynamic changes of mRNA expression of PCNA and Western blotting to protein expression of PCNA and cdk2 kinase. MTT assay was used to observe the changes of proliferation of cells. Results In the case of gradual elongation, the expression of ?-actin rose at first and fell afterwards; the mRNA expression of PCNA and protein expression of PCNA and cdk2 kinase rose gradually. Changes in 30% elongation group were greater than the control (P

0.05), but changed to be significant after stimulation in the rats stimulated respectively with formalin and saline (P

Objective To investigate the relationship between IP_(3)/Ca~(2+)signal transduction process and the changes of intracellular calcium in detrusor cells in rats.Methods The model of overactive detrusor(OAD) was successfully established in female Wistar rats.Intracellular calcium fluorescence intensity of cultured detrusor cells in OAD rats and detrusor stability(DS) rats was measured by laser scanning confocal microscopy(LSCM).After pretreated by 10~(-5) mol/L IP_(3 )alone or with its inhibitor heparin,the intracellular calcium fluorescence intensity of cultured detrusor cells of DS rats in Ca~(2+)-contained and Ca~(2+)-free solutions was measured with LSCM.Results Intracellular calcium fluorescence intensity of cultured detrusor cells in OAD rats was stronger than that of DS rats(P

Objective To study the innervation between baldder and rectum.Methods The retrograde fluorescent double labeling method was used.Propidium Iodide(PI)was injected into the right wall of the rectum of male SD rats and Bisbenzimide(Bb)into their right wall of urinary bladder.Results The fluorescent double labeled neurons were found in the right L6-S3 and L1-L3 dorsal root ganglia(DRG).Conclusion DRG cells at L6-S3 and L1-L3 simultaneously innervate the rectum and the urinary bladder,explaining that the abnormal micturition due to chronic constipation may be caused by axis reflex at the level of the peripheral process of DRG cells.

0.05), and there was no significant difference in instable contraction occurring at filling period. As compared with those before stimulation, the cervicitis model group had increased basic pressure and per-micturition pressure and significantly decreased micturition pressure, vesical volume and vesical compliance lowered (P0.05), but the changes were significant after stimulation (P

Objective To investigate the effects of prostatitis on secretory alterations of urethral glands in rats.Methods Twenty adult Sprague-Dawley rats were randomly divided into prostatitis group and control group.The prostatitis group(n=10) was injected with 0.1 ml Freund's complete adjuvant at each lateral lobe while the control group(n=10) with 0.1 ml 0.9% physiological saline.Two weeks later the rats were killed and the urethral glands were harvested for pathologic examination and immunohistochemical staining.Results ① There was a great quantity of lymphcells and mononuclear cells infiltrating the prostate gland in prostatitis group while there was no inflammatory cells in control group.② AB-PAS pathologic examination results in urethral glands showed that the grume area,diameter,optical density in control group were significantly less than those of the prostatitis group(P

Objective To study the causes, prophylaxis, and treatment of the severe complications due to transurethral electrovaporization of the prostate (TVP). Methods We analyzed the complications found in 1 910 men with moderate and severe symptoms of prostatism treated with TVP from August 1997 to February 2003 and studied the causes, prophylaxis, and treatment of the severe complications due to TVP. Results Severe complications were found in 78 cases (4.1%). Late mass bleeding in 35 cases (0.18%) was resulted from the abscission of coagulated necrotic tissue of the prostate after TVP. Extraperitoneal water extravasation (hydrocele) in 27 cases (0.14%) was caused by the early incision of the peritoneum in TVP and could be prevented by avoiding early incision of the peritoneum and stopping operation in time. Severe urethral stricture in 9 cases (0.05%) was caused by using thick sheath of cystoscope and cutting the neck of urinary bladder improperly. Urinary bladder explosion in 2 cases (0.01%) was caused by accumulation of air during the process of TVP, but could be protected by removing the air and water in urinary bladder at intervals. Incontinence in 2 cases (0.01%) was resulted from the injured urinary sphincter muscle. Acute renal failure in 3 cases (0.015%) was caused by hypotension. Conclusion Severe complications due to TVP can be prevented and treated. The occurrence rate of severe complications can be decreased by the proper preventive measures and skilled techniques of the operators.