Objective To evaluate early changes of femoral artery elasticity in type 2 diabetes mellitus (T2DM) combined with microalbuminuria (MA) patients using ultrasound radio-frequency technology,and to study the clinical significance.Methods 51 T2DM without MA patients were enrolled in Group A,and 53 T2DM with MA patients were enrolled in Group B.In order to observe the early elastic changes in the subjects,two-dimensional ultrasound was used to eliminate patients whose intima-media thickness (IMT) was ＞1 mm.The femoral arteries were imaged by two-dimensional ultrasonography,and then the IMT,femoral artery inner diameter (D),distensibility coefficient (DC),compliance coefficient (CC),elasticity coefficient (α and β),and pulse wave velocity (PWV) were real-time tested using quality intima-media thickness (QIMT) and quality arterial stiffness (QAS) analyzing systems.Besides,the differences and correlation analyses of these parameters between the two groups were conducted.In addition,some other parameters of sujects in both group A and B were also determined and analysed,including total cholesterol (TC),triglyceride (TG),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C),creatinine (CREA),glycosylated hemoglobin (HbA1c),fasting blood glucose (FPG),2h postprandial plasma glucose (2hPG) and body mass index (BMI).Results There were no significant differences in the elastic parameters of femoral arteries between the male and female subjects (P＞0.05),and between left and right sides (P＞0.05) within a same group.Compared with group A,group B had lower CC and DC (P＜0.05) and higher α,β and PWV (P＜0.05).Pearsons correlation analysis indicateal that,CC was negatively correlated with α,β and PWV (P＜0.05),and DC was also negatively correlated with α,β and PWV (P＜0.05).There was no significant difference in the inner diameters of femoral arteries between the two groups (P＞0.05).The levels of TG,HbA1c,FPG and 2hPG in group B were significantly higher than those in group A (P＜0.05).There were no significant differences in TC,HDL-C,LDL-C,CREA and BMI between the two groups (P＞0.05).Conclusions The decrease of arterial elastic function is earlier than morphological changes,that is,it may have occurred before MA in T2DM patients.The application of ultrasound radio-frequency analyzing technology has certain significance in the clinical early diagnosis and treatment.

OBJECTIVE: To study the appropriate time to take out T tube, long-term effect and adverse consequence of children with chronic otitis media subjected to adenoidectomy and tonsillectomy plus tympanostomy. METHOD: One hundred and eighteen children with 226 ears operated were analyzed and summarized. RESULT: T tubes taken out at 1, 3, 6 and 12 month were 6, 102, 88 and 23 ears, respectively, and T-tubes were not taken out in 7 ears. CONCLUSION At present, the treatment is curable and reliable with fewer complications. The appropriate time to take out the T-tube should be 3 months after the operation.
Adolescent ; Catheters, Indwelling ; Child ; Child, Preschool ; Chronic Disease ; Female ; Humans ; Male ; Otitis Media with Effusion ; surgery ; Time Factors ; Treatment Outcome

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) system is a hotspot of gene editing and gene expression research, in which CRISPR/Cas13 system provides a new direction for RNA interference and editing. In this study, we designed and synthesized the corresponding gRNAs of CRISPR/Cas13a and CRISPR/Cas13b systems in non-homologous end joining (NHEJ) pathway, such as Ku70 and Lig4, and then detected the expression of ku70 and lig4 in HEK293T cells. The CRISPR/Cas13a system could efficiently knockdown the mRNA expression of ku70 and lig4 more than 50%, and CRISPR/Cas13b system also suppressed ku70 and lig4 about 92% and 76%, respectively. Also, CRISPR/Cas13a, b systems could down-regulate Ku70 and Lig4 proteins level to 68% and 53%, respectively. The study demonstrates that the CRISPR/Cas13 system could effectively knockdown the expression of RNA and protein in HEK293T cells, providing a new strategy for gene function and regulation research.
CRISPR-Cas Systems ; DNA Ligase ATP ; genetics ; Gene Expression Regulation ; genetics ; Gene Knockdown Techniques ; HEK293 Cells ; Humans ; Ku Autoantigen ; genetics