Objective To investigate the application of different sizes of renal calculi retrograde intrarenal operation ( RIRS) in the clinical treatment effect.Methods 120 cases of the implementation of RIRS operation in patients with kidney stones were retrospectively analyzed, according to the size of stone were divided into A group (CM<2 stone) in 59 cases,B group (2-3 cm 41 cases calculi),group C (stone>3cm) in 20 cases,comparison of different size of kidney stones in patients with a period of stone clearance rate, operation time and complications. Results With the increase of stones, prolonged operation time, operation time of up to C group ( 172.66 ± 13.21) min;C group for a period of stone clearance rate was 45%,significantly lower than those of A group 88.14%and 82.93%in the B group,the difference was statistically significant (χ2 =5.294,6.223,all P<0.05);group A and B group of stone clearance rate had no significant difference ( P >0.05 );C group, the complication rate was 25%,9.76%in B group,A group was 5.08%,A group,B group complications incidence was statistically significant difference (χ2 =4.048,P=0.034),B group,C group complications had significant difference (χ2 =9.654,P=0.007),A group,C group complications had significant difference (χ2 =11.421,P=0.002).Conclusion Retro-grade intrarenal operation has a good stone clearance rate of diameter <3 cm stones,stones less than 2 cm of patients with postoperative complication rate is lowest,the effect is better.

Objective To investigate the effects of medicine androgen deprivation therapy(ADT)on quality of life in patients with prostate cancer.Methods A total of 42 consecutive advanced prostate cancer patients without any other anti-androgen medications after ADT[A subcutaneous depot injection of LHRH-agonist(Zoladex) was instituted every 28 days]were enrolled.Levels of serum testosterone and prostatic specific antigen(PSA)were obtained just prior to ADT and after ADT.The general and disease-specific health-related quality of life were assessed.Results The average testosterone level was less than 50 mg/L after medical ADT in 3 weeks.And PSA level declined dramatically in one month.Although there were no significant differences on physical discomfort and limitations to daily activities.Urinary obstruction symptoms after ADT were disappeared and the size of prostate were reduced after one month.The appetite and vigor were worsened.Overall health status and sexual function were significantly reduced.Conclusion The ADT could make serum testosterone and PSA decline dramatically in short time,and worsen some general health-related quality of life.

Objective: To screen and identify serum amyloid A (SAA) in patients with prostate cancer with mass spectrum technique. Methods: SELDI technology was used to detect the changes in protein expression. SAA was screened and separated and then identified by peptide mass fingerprint (PMF) based on matrix-as-sisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database searching. Results: The analysis showed that a mass cluster in the ranges of 11.1-11.9KD in M/Z value in the serum of prostate cancer patients was much higher than that in the control group. Meanwhile, this protein peak was closely correlated with clinical stages of prostate cancer. The level of the protein peak was increased as the illness got serious. Through MALDI technology combined with HPLC, the mass cluster in the range of 11.1-11.9KD in M/Z value on the chip was identified as SAA. And it was also verified through ELISA method. Conclusion: Mass spectrum technology is an effective method to detect the biological markers in prostate cancer patients. This method is convenient, highly sensitive and with good reproducibility. The SAA can be used as a marker in the diagnosis of prostate cancer. These indices are also meaningful in screening and identifying signal proteins from the serum of prostate cancer patients.

Objective To explore the clinical effects of different types of transurethral resection of prostate in the treatment of benign prostatic hyperplasia(BPH)complicated with diabetes mellitus.Methods Ninety-four patients with BPH complicated with type 2 diabetes mellitus admitted to Taizhou Municipal Hospital from January to December 2021 were randomly divided into control group and observation group,with 47 cases in each group.The control group underwent transurethral vaporization resection of prostate,and the observation group underwent transurethral plasmakinetic resection of prostate.The blood sugar,nerve growth factor(NGF),urodynamic indexes and complications of two groups were compared.Results During the operation,the blood sugar in observation group was significantly lower than that in control group(P<0.05).The rate of NGF positive cells in observation group was significantly lower than that in control group(P<0.05).One month after operation,the maximum urine flow rate and bladder compliance in observation group were significantly higher than those in control group,and residual urine volume was significantly lower than that in control group(P<0.05).Within one month after operation,the incidence of complications in observation group was significantly lower than that in control group(P<0.05).Conclusion Transurethral plasmakinetic resection of prostate for BPH patients complicated with diabetes mellitus can reduce the influence of operation on blood sugar,improve detrusor function and urodynamics,improve the therapeutic effect and reduce the incidence of complications.