Objective To evaluate the effect of beat to beat alterations in the cardiac cycle length on systolic flow velocity patterns of the pulmonary vein in patients with atrial fibrillation(AF).Methods Pulmonary vein flow was recorded in 20 control subjects and 74 patients with AF (16 with nonvalvular AF,22 with mild to middle mitral stenosis,and 36 with severe mitral stenosis) by transesophageal echocardiography.The peak velocities of the early systolic reversal wave (PVR) and the systolic forward wave (PVS) were measured.The relationships between the preceding R-R intervals and PVR,PVS were analyzed.Results The early systolic reversal wave was detected in all patients with AF.PVR was significantly lower in patients with severe mitral stenosis than that in patients with nonvalvular AF and mild to middle mitral stenosis.The preceding R-R interval had a significant negative correlation with PVR.PVS was significantly lower in patients with AF than that in controls.The preceding R-R interval had a significant positive correlation with PVS.Conclusions PVR and the systolic forward wave vary with disease conditions and preceding R-R intervals in atrial fibrillation.The evaluation of systolic pulmonary venous flow velocity patterns provides important information about left atrial hemodynamic abnormalities.

Objective To evaluate short-term efficacy, predictability, and rotational stability of the Acrysof SN60TT toric intraocular lens (IOL). Design Prospective case series. Participants 25 senile or evolutionary cataract patients (30 eyes) with more than 1.0 diopter (D) of preexisting corneal astigmatism. Methods The type and aim-axis of IOL was calculated using calculator software (date from biometry). The site of incision and aim-axis of IOL was marked before operation. Phacoemulsification was performed, and an Acrysof SN60TT toric IOL was implanted and rotated into the astigmatic axis. All patients were followed up for 3 months. Main Outcome Measures Uncorrected visual acuity (UCVA), best corrected visual acuity(BCVA),refraction, corneal astigmatism, and cylind er axis of the toric IOL. Results After the implantation of the Acrysof SA60TT toric intraocular lens, the UCVAs were 0.73?0.18 (1 month), 0.79?0.17 (3 months). The BCVAs were 0.93?0.13 (1 month), 0.98?0.09 (3 months). The refractive astigmatisms were 0.68?0.53 D (1 month), 0.47?0.39 D (3 months). The surgically induced astigmatism was 0.62?0.32 D. Astigmatism reduced from IOL was 1.27?0.69 D, compared to predicted 1.36?0.44 D (P=0.31). The axis alignment error was 4.0?3.2 degrees. At the final visit, 76.7% of the IOLs were within 5 degrees of the intended axis. Conclusion Implantation of Acrysof SA60TT toric IOL for correction of astigmatism in short-term is effective and stable. This IOL is showed no significant rotation postoperatively with rotational stability in the capsular bag.

AIM: To establish the experimental acute pulmonary embolism(APE) model and observe the left and right ventricular pressure-volume relationship in different overload situations. METHODS: The present study consisted of seven anesthetized mongrel dogs that were divided into the control group, moderate APE group and severe APE group according to the various phase and different pressure load during the experiment. APE model was induced by suture piece injection through right cardiac catheterization. The hemodynamic indexes were measured by the simultaneous cardiac catheterization and echocardiography.RESULTS: (1) In the group with moderate APE, the pressure-volume relationship of right ventricle tended to right-upward shift, the area of chart increased, the shape of chart transformed form triangle to rectangle. The mild parallel leftward shift, the area of chart decreased mildly and no change of chart shape was observed in the pressure-volume relationship of left ventricle. (2) In the group with severe APE, the chart of right ventricular pressure-volume relationship tended to right-upward shift continuously, the area of chart decreased. The chart of the left ventricle tended to left-downward shift and no change of chart shape was observed in the pressure-volume relationship of left ventricle, the area of chart decreased. The erose shape of charts was also found.CONCLUSION: The chart of ventricular pressure-volume relationships is a practical and reliable method to evaluate left and right ventricular hemodynamic in APE.

Objective To investigate the mechanisms of quercetin in retinal angiogenesis via in vitro and in vivo studies.Methods Human umbilical vein endothelial cells (HUVECs) was used in in vitro study,and oxygen-induced retinopathy (OIR) and laser-induced choroidal neovascularization (CNV) animal models were used in in vivo study.In the in vitro cell study,normal control group,VEGF-165 treatment group and VEGF-165 combined with quercetin treatment group were used.Cell counting kit-8 (CCK8) and Transwell were used to measure HUVECs cell viability,including proliferation and migration.OIR and CNV animal models were administrated through intraperitoneal injection of quercetin,and the non-perfusion area and CNV aera were evaluated.Western blot assay was used to detect the expression of integrin α5 and integrin β3 expression.In the animal study,18 breastfeeding mice and 18 infant mice were randomly divided into normal control group,model control group and quercetin treatment group,6 for each group.The animal feeding and use was in accordance with the standards set by the ARVO,and the experiment was approved by the Ethic Committee for Experimental Animal of Fu Xing Hospital,Capital Medical University (2016-KY-0036).Results In the in vitro study,VEGF-165 (20 ng/ml) promoted the proliferation and migration of HUVECs,while quercetin (50 μmol/L) inhibited HUVECs proliferation and migration significantly,comparing to the VEGF-165 treatment group (proliferation:Fgroups =18.51,P =0.00;migration:F =85.74,P =0.00).In the in vivo studies,quercetin 20 mg/(kg·day) decreased the non-perfusion area and CNV area comparing to the untreated groups,with significant differences between them (t =6.02,P =0.00;t =5.79,P =0.00).Besides,quercetin down-regulated the expression of integrin α5 and integrin β3 significantly both in the in vitro and in vivo studies.Western blot test showed that integrin α5 and integrin β3 in VEGF-165+quercetin processing 24 hours group were significantly decreased than those in the VEGF-165 group,with significant differences between them (t =4.46,P＜0.05;t =5.18,P＜0.01).Compared with the VEGF-165 +quercetin 24 hours group,the integrin α5 and integrin β3 in VEGF-165 +quercetin processing 48 hours group were increased,but they were still significantly increased than those in the VEGF-165 group,with significant differences between them (t =6.54,P ＜ 0.05;t =7.17,P ＜ 0.01).For the animal studies,quercetin also inhibited the levels of integrin α5 and integrin β3 in OIR and CNV models (t =5.44,13.52;both at P=0.00).Conclusions Quercetin can inhibit the retinal and choroidal neovascularization through integrin pathway,which provides a new treatment strategy for clinical therapy.

Animals ; Antigens, Surface/genetics* ; Cell Communication/genetics* ; Copulation/physiology* ; Fallopian Tubes/metabolism* ; Female ; Fertilization/genetics* ; GPI-Linked Proteins/genetics* ; Gene Expression Regulation ; Genes, Reporter ; Green Fluorescent Proteins/metabolism* ; Litter Size ; Luminescent Proteins/metabolism* ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mitochondria/metabolism* ; Reproduction/genetics* ; Signal Transduction ; Sperm Count ; Sperm Motility/genetics* ; Spermatozoa/metabolism* ; Uterus/metabolism*