Introduction It grows a larch forest, in its meadow, edge and glade, bank of the river and thick bushes forests covering Khuvsgul, Khentii, Khangai, Gobi Altai, mountainous and fertile pastures, Mountain range and Mongol Altaic Mountain range. Upper part of the land of Gentiana barbata, its preparation is effective to release poisoning, reduce inflammation, support liver and gall procedure internal treatment and an application external are inflammation fast healing, recover transfer activities of cell membrane. Drug preparations extracted from upper part of the land is confirming cell membrane, to jaundice. Its upper part of the land are for fast healing scald and inflammation, gallbladder diseases, to jaundice in Mongolian traditional Medicine. Goal The aim is to study of phytochemical Gentiana barbata. Materials and MethodsWe performed the research taking material assistance of pharmacognozy laboratories in Monos university from 2014-2015. The quantitative and qualitative analyses of plant bioactive compounds were evaluated by Russian XI Pharmacopeia and the method according to Mongolian National Standard, mineral substances were evaluated using roentgen fluorescence method. Result of the study was developed by SPSS 16 and End notе programme.ResultThe quantitative and qualitative analyses of plant bioactive compounds of Gentiana barbata upper part of the land were evaluated alkaloid, tannin, coumarone, flavonoid and mineral substances were found. Conclusion It was carried out phytochemical investigation of the Gentiana barbata using spectrophotometer as a result we determined that contains 6.0 % of sum flavonoid, 15.7 % coumarone. Amount of biological active compound-tannins was 3.2 %, alkaloid 0.5 %. When content of mineral substances in upper part of the land of Gentiana barbata contained K 60.2 %, Ca 25.12%, S 6.7%, CL 5.8 %, Sr 0.09%, Fe 0.83%, Ba 0.73%, Mn 0.152%, Rb 0.058%, Cu 0.069%, Zn 0.207%.Key words: Gentiana barbata, phytochemical, mineral substances, flavonoid, and 5,7,3´-Trihydroxy-4´-methoxyflavon.