Purpose: To study effect of San Gen talkh medicine and San Gen granule medicine, on healthy rats immune system Method: In this study 32 male healthy Wistar rats were randomly divided into normal group, low dose group of San Gen talkh (SGTLD), high dose group of San gen talkh (SGTHD) and group of San Gen granule (SGG). In normal group rats were administrated 0.2m l/kg saline solution, in San gen talkh groups rats with low dose and high dose were administrated 0.85g/kg, 1.70g/kg San Gen talkh respectively and in San gen granule medicine groups rats 1.3 g/kg San gen granule medicine, once daily for 42 day. By Elisa method measured IL-10, IL-12, IL-17, IFN-γ and TGF-β in blood plasma and spleen tissue of rat. Result: In this experimental result, there was no significantly difference all measured cytokine of blood plasm (P>0.05) between healthy group and all medicine group. In spleen tissue, there was significantly increased IFN-γ (P<0.05) and TGF-β (P<0.05) in SGTHD group and was significantly increased IFN-γ (PO.05), IL-10 (P<0.01)and TGF-β (PO.001) in SGG group compared with normal group. Conclusion High doses San Gen talkh medicine and San Gen granule medicine can support cellular immune system, humoral immune system on healthy rats.