To investigate the safety of subconjunctivally injected mitomycin-C(MMC) as an adjunctive therapy following the glaucoma filtration surgery(GFS), various amounts of MMC have been given to rabbit eyes after the surgery. To the control group, balanced salt solution(BSS) was injected subconjunctivally and 0.05mg MMC dissolved in BSS was given to the I experimental group I, 0.1mg to the group II, and 0.2mg to the group III. When the concentration of MMC in aqueous humor and cornea were measured at 30 min after the administration of MMC by high performance liquid chromatography, while no MMC was detected in aqueous humor, 1.2 +/- 0.5 micro gram of MMC/g of corneal tissue was detected for group III and 0.3 +/- 0.1 micro gram/g was found in group II. No MMC could be detected in corneal tissues of group I. The scanning electron microscopy which was done on the third day after surgery, revealed the most serious destructive changes of corneal endothelium in group III, while slight changes in group II, and no alteration in group I and control group. Based on these observations, subconjunctival injection of low dose(0.05mg) of MMC may be safe as an adjunctive method to enhance the success rate of GFS.
Aqueous Humor ; Chromatography, Liquid ; Cornea ; Endothelium, Corneal ; Filtering Surgery* ; Filtration* ; Glaucoma* ; Microscopy, Electron, Scanning ; Mitomycin* ; Rabbits*

Intravascular papillary endothelial hyperplasia (Masson's hemangioma) is a disease characterized by exuberant endothelial proliferation within the lumen of medium-sized veins. In 1923, Masson regarded this disease as a neoplasm inducing endothelial proliferation, however, now it is considered to be a reactive vascular proliferation following traumatic vascular stasis. The lesion has a propensity to occur in the head, neck, fingers, and trunk. Occurrence within the abdominal cavity is known to be very rare, and especially in the liver, there has been no reported case up to date. The authors have experienced intravascular papillary endothelial hyperplasia of the liver in a 69-yr-old woman, and report the case with a review of the literature.
Aged ; Angiography ; Endothelium, Vascular/pathology ; Female ; Hemangioendothelioma/*pathology/surgery ; Human ; Liver/*blood supply/*pathology ; Necrosis ; Vascular Neoplasms/*pathology/surgery

OBJECTIVE: This study evaluates the effects of H-uvulopalatopharyngoplasty (H-UPPP) on endothelial function by endothelium dependent flow-mediated dilation(FMD) in patients with moderate to severe obstructive sleep apnea syndrome (OSAHS). METHOD: Forty-five healthy subjects and 49 patients with moderately to severe OSAHS who desired H-UPPP were prospectively enrolled. FMD was measured by high-resolution B-mode ultrasonography. All subjects participated in sleep studies. These studies were repeated 6 months after H-UPPP in patients with moderately to severe OSAHS. RESULT: There was significant difference in FMD between patients with moderately to severe OSAHS and healthy subjects at baseline (6.5 +/- 2.1)% vs (11.2 +/- 3.1)% (P < 0.01). FMD increased from (6.5 +/- 2.1)% to (9.7 +/- 2.7)% 6 months after H-UPPP in patients with moderately to severe OSAHS (P < 0.01). CONCLUSION FMD is decreased in patients with moderately to severe OSAHS, H-UPPP treatment of these patients leads to improvement of FMD.
Case-Control Studies ; Endothelium, Vascular ; physiology ; Humans ; Otorhinolaryngologic Surgical Procedures ; methods ; Palate ; surgery ; Pharynx ; physiology ; surgery ; Polysomnography ; Prospective Studies ; Sleep Apnea, Obstructive ; surgery ; Uvula ; surgery

Light microscopy and electron microscopic examination were carried out on the corneal buttons of two patients who required penetrating keratoplasty for treatment of corneal complication following the intraocular injection of silicone oil to repair recurrent retinal detachments in aphakic eyes. Light microscopic examination demonstrated increased cellularity and irregularity of collagen fibers of stromal layer, defect of endothelial cell layer and endothelial degeneration. Electron microscopy examination demonstrated marked decrease in endothelial cell population density, accompanied by flattening and thinning of the remaining cells and attenuation of cell borders. There were silicone droplets in the endothelial cell layer and collagenous layer posterior to endothelial layer. These findings are well correlated to clinical manifestation and are thought to be rather due to barrier effect of silicone oil than direct toxicity.
Adult ; Corneal Diseases/*chemically induced/pathology/surgery ; Descemet Membrane/ultrastructure ; Endothelium, Corneal/drug effects/surgery/ultrastructure ; Humans ; Keratoplasty, Penetrating ; Male ; Recurrence ; Retinal Detachment/surgery ; Silicone Oils/*adverse effects

When the continuous circular capsulorhexis (CCC) is being performed, the nucleus delivery using nucleus dislocation into the anterior chamber is safer and easier than using conventional "push and pull" method to maintain an intact lens capsule. This method include such procedures that after CCC, the nucleus being freed in the capsular bag by hydrodissection and hydrodelineation, then hooked with a Sinskey hook and drawn out to the anterior chamber by rotation. It may damage the corneal endothelium because of the manipulation in the anterior chamber. To investigate an effect of this method on the corneal endothelium, we performed two months time analysis of changes of the central corneal endothelial cell density (CECD) in two groups--a group with an extracapsular cataract extraction (ECCE) using nucleus dislocation into the anterior chamber and a group with a conventional ECCE. Eighteen eyes of 18 cataract patients who were operated on with ECCE using nucleus dislocation into the anterior chamber method, and ten eyes of 10 cataract patients who were operated on with a conventional ECCE method were included. The CECD was measured by specular microscopy, preoperatively, postoperatively at 1 month and 2 months. The average endothelial cell loss at postoperative 1 month was 7.20 +/- 2.98% in the experimental group and 7.88 +/- 2.93% in the control group, and at postoperative 2 months was 9.05 +/- 2.96% in the experimental group and 9.34 +/- 2.95% in the control group. The change in CECD between two groups was not statistically significant.
Adult ; Aged ; Anterior Chamber/*surgery ; Cataract Extraction/*methods ; Cell Count ; Endothelium, Corneal/*pathology ; Female ; Humans ; Lens Capsule, Crystalline/*surgery ; Lens Nucleus, Crystalline/*surgery ; Male ; Middle Aged

Lymphangiectasis(=acquirecl lymphangioma) ate dilated lymph caillaris and may be due to the obstruction of the lymphatic circulation from surgery, irradiation, chronic recurrent infection or trauma. The patient was a 5 years old girl who developed a straw-colcre, asymptomatic grouped vesicular mass resembling frog spawn measuring 9 cm in diameter the left axilla and arm, 2 months after the total excision of a previous lymphangiectatic mass. She had a history of previous open heart surgery at 2 years of age. Histopathologic findings showed large, irregularly shaped cysticalation lined by a single layer of endothelium in the superficial dermis. Immunohistochemical stainig showed vimentin positive and negative results in Factor VIII and HLA-DR antigen which was compatible with a lymphatic vessel.
Arm ; Axilla ; Child, Preschool ; Dermis ; Endothelium ; Factor VIII ; Female ; Heart* ; HLA-DR Antigens ; Humans ; Lymphangioma* ; Lymphatic Vessels ; Thoracic Surgery* ; Vimentin

Iridocorneal endothelial(ICE) syndrome is charaterized by unilateral glaucoma, abnormal corneal endothelium with proliferation, and iris stromal abnormalities. Uncontrolled glaucoma in ICE syndrome has been treated with filtering surgery, but the late failures occurred frequently. The medical records of nine patients with ICE syndrome that underwent glaucoma surgery were retrospectively reviewed. 6 eyes were progressive iris atrophy, and 3 eyes were Cogan-Reese syndrome. The success rates of primary trabeculectomy at 1 and 2 years were 57.1% and 33.3%, respectively. Ahmed glaucoma valve implant oeprations were performed on 6 eyes with failed previous filtering operation. 4 of 6 eyes resulted in controlled IOP until last follow up visit(mean ; 18.8 months). Overall, 1.9 times operations were needed to control IOP. Operations were performed only one time in 4 patients and two times in 3 patients to control IOP.
Atrophy ; Endothelium, Corneal ; Filtering Surgery ; Follow-Up Studies ; Glaucoma* ; Humans ; Ice ; Iridocorneal Endothelial Syndrome* ; Iris ; Medical Records ; Retrospective Studies ; Trabeculectomy

The endothelial glycocalyx (EG) is a gel-like layer lining the luminal surface of healthy vascular endothelium. Recently, the EG has gained extensive interest as a crucial regulator of endothelial funtction, including vascular permeability, mechanotransduction, and the interaction between endothelial and circulating blood cells. The EG is degraded by various enzymes and reactive oxygen species upon pro-inflammatory stimulus. Ischemia-reperfusion injury, oxidative stress, hypervolemia, and systemic inflammatory response are responsible for perioperative EG degradation. Perioperative damage of the EG has also been demonstrated, especially in cardiac surgery. However, the protection of the EG and its association with perioperative morbidity needs to be elucidated in future studies. In this review, the present knowledge about EG and its perioperative implication is discussed from an anesthesiologist's perspective.
Blood Cells ; Capillary Permeability ; Endothelium, Vascular ; Glycocalyx* ; Oxidative Stress ; Permeability ; Phenobarbital ; Reactive Oxygen Species ; Reperfusion Injury ; Thoracic Surgery

Diagnosis, Differential ; Endothelium, Vascular ; metabolism ; pathology ; Female ; Humans ; Hyperplasia ; Jejunal Diseases ; metabolism ; pathology ; surgery ; Jejunum ; blood supply ; pathology ; surgery ; Middle Aged ; Platelet Endothelial Cell Adhesion Molecule-1 ; metabolism

Three hundred and forty-eight eyes (246 patients, both eye radial keratototmy in 102 patients) which could be followed-up for at least one year or more were included in this study. Postoperative uncorrected visul acuity which was 20/40 or more could be obtained in 79% of the lower myopic eyes (-1.75--2.75 D), in 73% of the moderate myopic eyes (-3.00--5.75 D) and in 34% of high myopic eyes (-6.00 D-). The postoperative refractive correction (spherical equivalent) ranged from plano to -9.0 D with a mean decrease of -3.86 D with a mean reduction of keratometry in moderate myopia of 3.33 D with a range from 0.5 D to 6.75 D. But its mean reduction in high myopia did not parallel its myopic degree. Glare and fluctuation of vision were the most frequent complaints following surgery. Some patients had continuous constant vision improvement for a long period while some patients (or other eyes) had episodes of decreased vision because of the recurrence of myopia or hyperopia shift. The mean central corneal endothelial cell loss determined 6-l2 months later was 5.31-5.61%. Microperforation in 11 eyes (3%) occurred during the early part of the study and improved naturally without any problem. An over-correction of more than +1.0 D (2%) and an under-correction of more than -1.0 D (1%) with having induced or residual astigmatism (17%) were observed. Radial keratotomy is suceessful in carefully selected patients with mild and moderate myopia, and also in anisometropic high myopia.
Adolescent ; Adult ; Endothelium, Corneal/cytology ; Female ; Follow-Up Studies ; Humans ; Keratotomy, Radial/*statistics & numerical data ; Korea ; Male ; Myopia/*surgery ; Prospective Studies ; *Visual Acuity