1.Associations between urinary bisphenol A concentrations during pregnancy and gestational age
Jie HU ; Ying LU ; Wei MIAO ; Tong ZHOU ; Jian-qiu GUO ; En-mao CAI ; Chun-hua WU
Shanghai Journal of Preventive Medicine 2021;33(1):47-
Objective We aimed to assess the association between urinary bisphenol A(BPA)concentrations and gestational age in pregnant women. Methods A total of 248 pregnant women were recruited from a maternal and child care hospital in Shanghai. A questionnaire survey was completed to collect socio-demographic information and spot urine samples were collected during pregnancy. Gas chromatography-tandem mass spectrometry(GC-MS/MS)was used to measure BPA concentrations in urine samples. Linear relationship between urinary BPA level and gestational age was assessed by using generalized additive models. Multivariate regression model was used to evaluate associations of prenatal BPA exposure with gestational age. Results BPA was detected in all the urine samples. Median value and geometric mean of urinary BPA levels were 0.85 μg/L and 1.21 μg/L, respectively. Linear relationship between urinary BPA concentration and gestational weeks was confirmed(non-linear
2.Effects on the survival of rat's abdominal axial skin flap after the combination of VEGF gene therapy and skin flap delay.
Yan ZHANG ; Jun-Sheng MAO ; Bing-Chen WANG ; Kai ZHANG ; En-Dong SHI ; Yong-Jie LIN ; Kang-Mei HUANG ; Jin-Fang CAI
Chinese Journal of Plastic Surgery 2008;24(1):39-41
OBJECTIVETo investigate the effect of VEGF (vascular endothelial growth factor) gene therapy and skin flap delay on the survival of rat's abdominal axial skin flap.
METHODSIn 48 Wistar rats, the model of a abdominal axial skin flap supplied by right superficial epigastric vessel was created. The rats were divided into six groups. The group was treated with subcutaneous injection of pcDNA4-VEGF165, skin flap delay or VEGF injection combined with skin flap delay. 7 days later, the survival rate of the skin flap was measured; specimens were harvested from the skin flap for histological investigation of the microvessels and for immunohistochemical staining to observe the expression of VEGF.
RESULTSEvery treated group was significantly higher than blank group in the average survival rate of the skin flap and group V (gene injection when delayed) has the highest one. The average number of the microvessels in group II, III, V, VI was significantly higher than group IV and blank group. Group IV > group V, VI > group II, III > blank group in lumen diameter of the microvessels. Immunohistochemical staining documented more deposition of VEGF DNA in group II, III, V, VI than group IV and blank group.
CONCLUSIONSBoth administration of pcDNA4-VEGF165 and skin flap delay can improve the survival of rat's abdominal axial skin flap, but the mechanisms of the effect were different. The combination of the two ways has stronger effect.
Abdominal Wall ; surgery ; Animals ; Disease Models, Animal ; Genetic Therapy ; Genetic Vectors ; Graft Survival ; Male ; Rats ; Rats, Wistar ; Surgical Flaps ; Vascular Endothelial Growth Factor A ; genetics
3.Treatment of gastric varices by endoscopic sclerotherapy using butyl cyanoacrylate: 10 years' experience of 635 cases.
Liu-fang CHENG ; Zhi-qiang WANG ; Chang-zheng LI ; Feng-chun CAI ; Qi-yang HUANG ; En-qiang LINGHU ; Wen LI ; Guo-jun CHAI ; Guo-hui SUN ; Yong-ping MAO ; Yan-mei WANG ; Jing LI ; Ping GAO ; Tie-yan FAN
Chinese Medical Journal 2007;120(23):2081-2085
BACKGROUNDGastric varices (GV) are life-threatening for patients with portal hypertension. Endoscopic injection with butyl cyanoacrylate (BC), the mainstay of the therapy for GV, has been reported to be effective for hemostasis of bleeding varices, but its efficacy in the obliteration of GV and impact on the survival of patients still needs clarification. Here we summarized our experience of 10 years' practice to evaluate the efficacy and safety of endoscopic therapy using BC for GV patients.
METHODSFrom January 1997 to April 2006, GV cases treated with endoscopic injection using BC were collected. The "sandwich method" and the "modified sandwich method" were used to inject BC intravascularly. Retrograde analysis was made on the data of treatment and follow-up.
RESULTSA total of 635 GV cases treated with endoscopic injection using BC were collected, most of them (90.2%) suffered from post-hepatitis cirrhosis. Emergency hemostasis was achieved in 139 out of 146 sessions (95.2%). Complications occurred in 32 cases (5.2%), including hemorrhage due to early expulsion of tissue glue (3.1%), septicemia (1%) and ectopic thrombosis (0.5%), such as spleen infarction. Endoscopic follow-up in 503 patients showed complete disappearance (76.9%), collapse (17.3%) or remnants (5.8%) of gastric varices. A total of 550 patients were followed up clinically for 3 to 115 months. Of these patients, 44 had recurrent bleeding (8.0%) and 44 died from hepatic failure, recurrent bleeding, hepatic carcinoma or other causes. The longest survival was 115 months, with a median survival of 25 months. Survival rates at 1, 2, 3, 4 and 5 year were 95%, 92%, 90%, 83% and 81%, respectively.
CONCLUSIONSEndoscopic sclerotherapy with BC is effective for the hemostasis of bleeding GV, as well as obliteration of GV which contributes to less rebleeding and better survival. The modified sandwich method may be useful to minimize ectopic embolism, which we speculated to result from excess iodized oil.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Enbucrilate ; therapeutic use ; Endoscopy, Gastrointestinal ; methods ; Esophageal and Gastric Varices ; mortality ; therapy ; Female ; Humans ; Male ; Middle Aged ; Sclerotherapy ; adverse effects ; methods ; Tissue Adhesives ; therapeutic use
4.Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program.
Rong HAI ; Dong-zheng YU ; Xian-ming SHI ; Zhong-bing ZHANG ; Yong-jiao TANG ; Peng WANG ; Lian-xu XIA ; Shao-zhen WEI ; Bing XU ; Ying-xu QIN ; Zhi-kai ZHANG ; Guo-xiang SHI ; Xu DONG-LEI ; Hong CAI ; En-min ZHANG ; Jian-chun WEI ; Ying-zhi GENG ; De-hui HUANG ; Bin ZHAO ; Li-mao WANG ; Feng-qin MA ; Fu HUANG ; Yue WANG ; Tao ZHANG ; Jian-hua ZHANG
Chinese Journal of Epidemiology 2007;28(5):426-429
OBJECTIVETo apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.
METHODS1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.
RESULTSThe overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.
CONCLUSIONThe new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.
Animals ; Bacterial Proteins ; genetics ; immunology ; Enzyme-Linked Immunosorbent Assay ; Mice ; Plague ; microbiology ; Polymerase Chain Reaction ; Yersinia pestis ; genetics ; immunology ; pathogenicity
5.Comparative study of confirmed and suspected undiagnosed cases of COVID-19 in Changning District, Shanghai
Hui GAO ; Jia-ling GU ; Wen-sui ZHAO ; Hong TANG ; Yi XIA ; Gen-ming ZHAO ; En-mao CAI ; Jian-lin ZHUANG
Shanghai Journal of Preventive Medicine 2021;33(5):453-457
Objective:To analyze and compare the differences between the epidemiological data and clinical indicators of confirmed and suspected undiagnosed cases of COVID-19 in Changning District, Shanghai. Methods:A retrospective comparative study was conducted. We included 20 confirmed and 34 suspected but undiagnosed COVID-19 cases from January 20 to February 29, 2020. We analyzed the differences in epidemiological history, early clinical symptoms, blood routine indicators, and clinical imaging characteristics between the two groups. Results:The epidemic status of COVID-19 in Changning District of Shanghai was mainly imported, and most cases were promptly confirmed. Early clinical symptoms of confirmed and suspected undiagnosed cases often manifested as respiratory symptoms such as fever and dry cough. Compared with the confirmed cases, the cell counts of leukocytes, eosinophils, and neutrophils in suspected undiagnosed cases were significantly higher. Also, the concentration of serum C-reactive protein in suspected cases was higher than that in confirmed cases (
6.Investigation and clarification of traditional measuring units of Tibetan medicine.
Qi-En LI ; Di-Gao WAN ; Fa-Rong YUAN ; Cai-Jia SUONAN ; Dai-Ji QINGMEI ; Yang-Xiu-Cuo DUOJIE ; Zhuo-Ma GENGJI ; Cuo-Mao TABA ; Peng-Cuo DAWA ; Zhong BANMA ; Cai-Rang DUOJIE ; Qu-Pei DANZENG ; Ci-Ren NIMA ; Xiao GUO
China Journal of Chinese Materia Medica 2023;48(5):1393-1401
Quantity is the key factor to ensure the safety and effectiveness of medicines. It is very important to study and determine the traditional measuring units and their quantity values of Tibetan medicine. Based on the literature records of Tibetan medicine and combined with modern experimental verification and investigation research, this study determined the reference, name, and conversion rate of traditional measuring units of Tibetan medicine. Meanwhile, through large sample sampling and repeated quantification of refe-rence of basic units, its weight and volume were clarified. The modern SI volume and weight unit values corresponding to the traditional volume and weight units of Tibetan medicine were deduced, and the correctness, reliability, and practicability of these determination results were demonstrated. This study also put forward some specific suggestions and reference values for formulating the standards of measuring units of weight and volume of Tibetan medicine. It is of great significance in guiding the processing, production, and clinical treatment of Tibetan medicine, and promoting the standardization and standardized development of Tibetan medicine.
Medicine, Tibetan Traditional
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Reproducibility of Results