1.Survey of the influencing factors of learning adaptability in nursing student of the emergency department
Xiaohong LIU ; Qi YU ; Fei′en CHEN ; Xiaoyan LIN ;
Modern Clinical Nursing 2015;(4):9-11,12
Objective To investigate the influencing factors of learning adaptability of nursing student in the emergency department and come up with relevant countermeasures. Methods Two hundred nursing student in the emergency department participated in the survey. A self-designed questionnaire on their learning adaptability and the results were analyzed. Result The job category, work environment, disease of the patients, internship time, teaching method and the professional identification were the top six factors influencing their learning adaptability situation. Conclusion Such measures as strengthening the orientation of student nurses, cultivating their observation ability and communication skills, enhancing the consciousness about law, increasing practice time appropriately, improving teaching methods and ability, and strengthening sense of identity can guarantee effective internship and adaptability of the nursing student in emergency department.
2.Effect of combined therapy of mild hypothermia and hibernation on severe brain injury
Yi-hua AN ; En-zhong LIU ; Chun-jiang YU ; Zhanqiang HAN
Chinese Journal of Rehabilitation Theory and Practice 2004;10(3):181-182
ObjectiveTo investigate the efficacy of combined therapy of mild hypothermia and hibernation to treat severe brain injury. Methods24 patients with severe brain injury were randomly divided into combined therapy group and normothermia group. Glasgow Coma Scale scores of all the patients were in the range of 3 to 8. No later than 10 hours after their injury, hypothermia patients were given half dosage of No.1 hibernation cocktail and had been cooled by cooling blankets to 32℃-34℃ (rectal temperature) for 5 days, then to 35℃ for 24 hours, and slowly increased to their normal level. 3 days and 7 days after their admission, intracranial pressure,creatine phosphate kinase,partial pressure of arterial O2 and CO2, platelet and Na+,K+ were measured.7 days after their admission, Glasgow Outcome Scale scores of each patient and mortality of each group were measured. ResultsThe mortality of combined therapy group(25.0%) was significantly lower than that of normothermia group (66.6%,P<0.05). The decreased values of intracranial pressure, creatine phosphate kinase and platelet number of combined therapy group were all significantly higher than that of normothermia group respectively (P<0.05). There were no significant difference in mean artery pressure, blood electrolyte, and partial pressure of arterial O2 and CO2 between these two groups(P>0.05). ConclusionThe combined therapy of mild hypothermia and hibernation can effectively reduce the mortality of patients with severe brain injury as it is much easier, less invasive and with less complications.
3.Surveillance for Respiratory Viruses in Children with Acute Lower Respiratory Infections in Chongqing between 2003 and 2007
dong-hong, PENG ; en-mei, LIU ; xiao-dong, ZHAO ; ying, HUANG ; yu, LIU ; xiao-ju, LUO
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To investigate the clinical spectrum of respiratory viruses in infants and young children with acute lower respiratory infection(ALRI) in Chongqing area from 2003-2007.And to assess the clinical diagnostic value of virus detection in nasopharyngeal secretions(NPS) and serum viral antibody detection for ALRI.Methods Cases of 2 529 specimens of NPS in hospitalized children with ALRI from Apr.2003 to Oct.2007 were taken for detecting 7 common respiratory virus antigens by immunofluorescence assay including respiratory syncytial virus (RSV),adenovirus(ADV),influenza A(IA),influenza B (IB),parainfluenza virus1-3 (PIV1,PIV2,PIV3).Fifty-five thousand eight hundred and eighty-seven samples were tested for ADV-IgM by ELISA.Among those,45 159 cases were further tested for RSV-IgM by ELISA.Results Respiratory virus pathogens were detected in 778 samples out of 2 529(30.76%) including RSV positive in 668 samples (85.86%),PIV3 positive in 75 samples (9.64%),IA positive in 22 samples (2.57%),ADV positive in 15 samples ( 1.93%),only 1 sample ( 0.13%) positive for both PIV1 and RSV. And the positive rate of RSV-IgM was 0.9%-15.2%,and the positive rate for ADV-IgM was about 0.6%-10.6%.RSV infection occured mainly in winter and spring.Conclusions Respiratory virus is the most common pathogen in children with ALRI during the survey period in Chongqing area,especially for RSV infection.The pattern of RSV circulation varied every year with seasonality.It is suggest that this year is peak one for RSV infection from the monthly positive results,especially in Feburary(50%) in 2007.But the infection rate of PIV3,IA,ADV and PIV1 are lower,particularly IB and PIV2 infection have not been seen for the last 5 years.It is fast and accurate to detect RSV antigen and suit to clinical diagnosis by using immunofluorescence assay than other antibody detection.
4.Effect of Recurrent Severe Hypoglycemia on Cognitive Performance in Adult Patients with Diabetes: A Meta-analysis
CHEN YU-XUE ; LIU ZHENG-REN ; YU YING ; YAO EN-SHENG ; LIU XING-HUA ; LIU LU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2017;37(5):642-648
The purpose of this study was to investigate the existence and extent of cognitive impairment in adult diabetes mellitus (DM) patients with episodes of recurrent severe hypoglycemia,by using meta-analysis to synthesize data across studies.PubMed,EMBASE and Cochrane library search engines were used to identify studies on cognitive performance in DM patients with recurrent severe hypoglycemia.Random-effects meta-analysis was performed on seven eligible studies using an inverse-variance method.Effect sizes,which are the standardized differences between the experimental group and the control group,were calculated.Of the 853 studies,7 studies met the inclusion criteria.Compared with control subjects,the adult DM patients with episodes of recurrent severe hypoglycemia demonstrated a significantly lowered performance on memory in both types of DM patients,and poor performance of processing speed in type 2 DM patients.There was no significant difference between adult DM patients with and those without severe hypoglycemia in other cognitive domains such as general intelligence,executive function,processing speed and psychomotor efficiency.Our results seem to confirm the hypothesis that cognitive dysfunction is characterized by worse memory and processing speed in adult DM patients with a history of recurrent severe hypoglycemia,whereas general intelligence,executive function,and psychomotor efficiency are spared.
5.Change of matrix metalloproteinase-9 in rats with multiple organ dysfunction syndrome
Min YU ; Guanlan LI ; Xianzhe LIU ; Min QIAN ; Xinli ZHANG ; Yuqi WU ; En LV ; Changyun GUO
Chinese Journal of Emergency Medicine 2009;18(2):165-169
Objective To investigate the changes in the serum MMP-9 (matrix metalloproteinase-9) and the expressions of MMP-9 in lung, kidney and intestine in rats with multiple organ dysfunction syndrome (MODS) and confirm extracellular matrix injuries being the mechanism in MODS in order to propose a novel theoretical basis for cfinical treatment of MODS. Method Forty wister rats were randomly divided into two groups: control group (n=8) and MODS model group (n=32). The rats of model group were further divided into four subgroups ac-cordingto the time elapsed after modelling: 12 h (n=8), 24 h(n=8) ,48 h(n=8) and 72 h (n=8), and were modelled by celiac injection of mixed liquid of zymosan-paraffin (4 mL/100 g) after blood loss (1mL/100 g) by extirpating their left eyes. Blood,lung, kidney and intestine were sampled 12,24,48 and 72 hours after models were established. The histological changes in the lung, kidney and intestine of the rats were observed by light mi-croscope. The serum MMP-9 were measured by enzyme-linked immunosorbent assay (ELISA). The immunohisto-chemistry was used to observe the expression of MMP-9 in lung,kidney and intestine during different phases of MODS. The data were processed by one-way ANOVA and Bivariate analysis. Results Compared with control group, the organs were injured by congestion, edema and inflammatory cells infiltration to a certain extent in model groups. The serum MMP-9 increased markedly 12 hours after modelling (P<0.01 ) and peaked 48 hours later. The expressions of MMP-9 in lung, kidney and small intestine significantly increased from 12 h to 72 h after mod-elling (P<0.01 or 0.05). Conclusions The MMP-9 increased both in serum and tissue are closely associated with the pathological process of MODS. The mechanism of organ damage probably attributes to the damage of extra-celluar matrix and tissue construction.
6.Long-term outcomes of endoscopic submucosal dissection for gastric stromal tumors
Changji YU ; Chaoqiang FAN ; Jin YU ; Xia XIE ; Xubiao NIE ; Xinwei DIAO ; En LIU ; Jianying BAI ; Shiming YANG
Journal of Regional Anatomy and Operative Surgery 2016;25(4):279-282
Objective To explore the long-term outcomes of endoscopic submucosal dissection (ESD) of gastric GISTs.Methods Data of 25 patients with gastric stromal tumors,who underwent ESD,were reviewed in terms of clinical characteristics,histopathologic results,complications and long-term outcomes.Results Twenty-five patients underwent one-time complete removal of lesion, with a average tumor size of 3.0 cm (range,0.6 to 7.6 cm).The average time of operation was 65 minutes (range,15 to 154 minutes).one case was observed with intraoperative hemor-rhage of 200 mL blood,which was successfully managed by hemostatic forceps.Perforation with incidence rate of 16%occurred in 4 cases and was closed well with clips.Mucosal mechanical laceration of esophagus occurred in 1 case when the big tumor was taken out.The average length of hospi-talization was 7.3 days (range,4 to 21 days).A follow-up for 30 months (range,13 to 54 months) showed no tumor recurrence or metastasis. Conclusion ESD is an effective and safe endoscopic procedure to remove gastric GISTs for long-term outcomes in patients with no metastasis.
7.Changes of metabotropic glutamate receptor subtype 1a in diffuse brain injury with secondary brain insults and the effects of 2-methyl-4-carboxyphenylglycine.
Zhou FEI ; Xiang ZHANG ; En-Yu LIU
Chinese Journal of Traumatology 2003;6(5):270-274
OBJECTIVETo observe the changes of metabotropic glutamate receptor 1a in rat brain in a rodent model of diffuse head injury with secondary insults and the effects of 2-methyl-4-carboxyphenylglycine (MCPG).
METHODSBased on Marmarous rodent model of diffuse brain injury (DBI), hypotension was made by blood withdrawal as secondary brain insults (SBI). 105 male SD rats were randomized into A and B groups. The changes of mGluR(1a) in cerebral cortex were studied by immunohistochemistry and the effect of MCPG by HE. Each group was divided into different subgroups at different time after injury.
RESULTSCompared with that of sham group, the number of mGluR(1a) positive neuron increased by 12.9+/-3.2 (P<0.05) 1 day after injury in the injured cerebral cortex in DBI group. However, in DBI and SBI group there was a more significant increase in the number of mGluR(1a) positive neuron at 4 hours after injury (15.6+/-3.0, P<0.05) and then the number of mGluR(1a) positive neuron gradually decreased. Administration of MCPG reduced total cortical necrotic neurons counts on the 7th day after injury (5.21+/-2.52, P<0.05).
CONCLUSIONSBrain injury can increase the gene expression of mGluR(1a) and the role of mGluR(1a) may be a key factor in the aggravation of head injury with SBI, and that MCPG may have therapeutic potential in head injury.
Analysis of Variance ; Animals ; Benzoates ; pharmacology ; Blood Pressure ; drug effects ; Brain Injuries ; metabolism ; physiopathology ; Glycine ; analogs & derivatives ; pharmacology ; Male ; Rats ; Rats, Sprague-Dawley ; Receptors, Metabotropic Glutamate ; metabolism
8.Effect of flurbiprofen on preemptive analgesia in teeth extraction under intravenous sedation by midazolam.
Ming GUAN ; En-Bo WANG ; Nian-Hui CUI ; Yu LIU ; Bei DING ; Wei ZHANG
Chinese Journal of Stomatology 2013;48(9):554-555
Adolescent
;
Adult
;
Analgesics
;
administration & dosage
;
Anesthetics, Intravenous
;
administration & dosage
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Flurbiprofen
;
administration & dosage
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Humans
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Hypnotics and Sedatives
;
administration & dosage
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Midazolam
;
administration & dosage
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Middle Aged
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Molar, Third
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Pain Measurement
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Pain, Postoperative
;
prevention & control
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Patient Satisfaction
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Preoperative Care
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Prospective Studies
;
Tooth Extraction
;
Young Adult
9.Protective effects of total flavones of metasequosia on cerebral ischemia-reperfusion injury in rats.
Fang WANG ; En-xin YU ; Wei-wan LIU
China Journal of Chinese Materia Medica 2004;29(2):179-181
OBJECTIVETo observe the protective effects of total flavones of metasequosia (TFM) on cerebral ischemia-reperfusion injury in rats.
METHODThe bilateral vertebral arteries of rats were occluded under anesthesia, and 4-5 h later the carotid arteries of rats in the conscious condition were occluded for 30 min followed by reperfusion for 90 min. The effects of TFM on the contents of water, Na+, Ca2+ in cortex, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in brain hemispheres, as well as the EEG activities were observed.
RESULTAfter 30 min ischemia and 90 min reperfusion, the contents of water, Na+ , Ca2+ and MDA were increased, and the SOD activity was reduced with abnormal EEG activity and ischemic injury in the brain tissues. TFM 25-100 mg x kg(-1) ip 30 min before the carotid arteries were occluded, decreased the elevated water, Na+, Ca2+ and MDA contents, increased the SOD activity, reduced the ischemic injury of brain tissue, and promoted the recovery of EEG activities.
CONCLUSIONTFM has a protective effect on cerebral ischemia-reperfusion injury in rats.
Animals ; Brain ; metabolism ; physiopathology ; Brain Ischemia ; complications ; Electroencephalography ; drug effects ; Flavones ; isolation & purification ; pharmacology ; Male ; Neuroprotective Agents ; isolation & purification ; pharmacology ; Plants, Medicinal ; chemistry ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; etiology ; metabolism ; pathology ; Taxodiaceae ; chemistry
10.Effects of extremely low frequency electromagnetic fields on apoptosis and cell cycle of mouse brain and liver cells.
Yun LIU ; Rong HONG ; Yun-mei YU ; En-qi WENG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(5):339-341
OBJECTIVETo study the effects of extremely low frequency electromagnetic fields (ELF EMFs) on apoptosis and cell cycle of mouse brain and liver cells.
METHODSMice were exposed to 50 Hz, 0.2 mT or 6.0 mT electromagnetic fields for 2 weeks. TUNEL and flow cytometric methods were used to analyze apoptosis and cell cycle of brain and liver cells.
RESULTSAfter exposure to 0.2 mT and 6.0 mT ELF EMFs for 2 weeks, apoptosis rates of brain cells [(5.60 +/- 1.47)% and (4.73 +/- 0.48)% respectively] were higher than that of control [(2.90 +/- 0.75)%], and apoptosis rates of liver cells [(4.19 +/- 2.08)% and (3.38 +/- 0.65)% respectively] were higher than that of control [(1.84 +/- 0.76)%]. G0/G1 cell percentage of brain cells [(80.21 +/- 1.68)% and (79.54 +/- 0.56)% respectively] were higher than that of control [(76.85 +/- 0.83)%], and those of liver cells [(79.42 +/- 1.80)% and (80.47 +/- 1.79)% respectively] were higher than that of control [(73.36 +/- 3.10)%]. The above differences were all statistically significant as P < 0.05. At the same time S and G2 + M cell percentage of brain and liver cells were significantly decreased.
CONCLUSIONExposure to 50 Hz EMFs may alter cell cycle and induce apoptosis of mouse brain and liver cells.
Animals ; Apoptosis ; radiation effects ; Brain ; cytology ; radiation effects ; Cell Cycle ; radiation effects ; Electromagnetic Fields ; Flow Cytometry ; In Situ Nick-End Labeling ; Liver ; cytology ; radiation effects ; Male ; Mice