Neuroglobin (Ngb) is an oxygen-carrying globin that specifically expresses in brain tissue.It is involved in energy metabolism,mitochondrial function,as well as cell survival and proliferation of signaling pathway regulation.Under physiological conditions,Ngb presents as a form of ferrous deoxy hexacoordination,which has stronger oxygen affinity.During ischemia and hypoxia,the expression of Ngb is upregulated in brain tissue and interacts through a variety of proteins of its downstream,and plays a protective role for the damaged brain tissue.

OBJECTIVE: To compare the pharmacokinetics of total glucosides of paeony (TGP) in rats with acute liver injury and normal rats, thus to provide evidence for determining dosage regimen in clinic. METHODS: Acute liver injury rat model was established by single CCl4 intraperitoneal injection. Three dosages of TGP were orally administered to normal and model rats. The concentrations of Pae and Alb in plasma at different time after dosing were determined with HPLC method. The pharmacokinetic parameters were calculated based on mean plasma concentration-time curves of Pae and Alb. RESULTS: In model rat group, the t1/2 of Pae and Alb at high, medium, and low dosage (0.047, 0.141, 0.282 g · mL-1) were 5.02, 4.52, 4.91 h and 5.22, 5.04, 5.05 h, respectively. The ρmax of Pae and Alb of TGP at different dosages were 22.54, 14.52, 5.49 μg · mL-1, and 9.02, 5.04, 2.34 μg · mL-1, respectively. The AUC0-t of Pae and Alb of TGP at different dosages were (146.55 ± 7.52), (84.14 ± 7.84), (31.62 ± 2.97) μg · h · mL-1, and (56.56 ±8.70), (32.18 ± 3.49), (13.48 ±1.66) μg · h · mL-1, respectively. The tmax of paeoniflorin(Pae) and albiflorin(Alb) of TGP at different dosages were both 2 h. In rats of normal group, the t1/2 of Pae and Alb of TGP at different dosages respectively were 3.95, 3.69, 3.95 h, and 3.74, 3.98, 3.79 h, respectively. The ρmax were 19.16, 11.60, 4.46 μg · mL-1, and 7.16, 4.00, 1.93 μg · mL-1, respectively. The AUC0-t were (116.26.6 ± 19.99), (67.74 ± 12.79), (25.01 ±2.62) μg · h · mL-1, and (45.03 ±5.84), (27.26 ±3.57), (10.59 ± 1.86) μg · h · mL-1, respectively. The tmax were both 2.5 h. CONCLUSION: Compared with normal group, the ρmax and AUC0-t of TGP in model group significantly increased, tmax decreased, and t1/2 prolonged; tmax and t1/2 had no difference among three dosages, and ρmax. and AUC0-t had correlation with dosage. Copyright 2012 by the Chinese Pharmaceutical Association.

Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Silicosis ; diagnostic imaging ; Tomography, Spiral Computed

Objective: To investigate the effects of rosuvastatin on reactive oxygen species (ROS) production and periostin, cardiotrophin-1 (CT-1) expression, and to explore rosuvastatin on ventricular remodeling in experimental rats after acute myocardial infarction (AMI). Methods: A total of 45 male Wistar rats were randomly divided into 2 group, Sham operation group,n=15 and AMI group,n=30, the AMI model was established by left anterior descending coronary ligation. After 24 hours of AMI, the rats were further divided into 2 groups, AMI + rosuvastatin group, the rats received gastric rosuvastatin 1mg/(kg?d), and AMI group, the rats received gastric normal saline.n=15 in each group and all animals were treated for 6 weeks. The mRNA and protein expressions of CT-1 and periostin were examined by real time RT-PCR and immunohistochemistry, the contents of superoxide anion (O2-·) and hydroxy radical (OH·) were detected by colorimetric method among different groups. Results: Compared with Sham operation group and AMI + suvastatin group, the mRNA and protein expressions of CT-1, periostin, the contents of (O2-·), (OH·) and left heart weight index were increased in AMI group at non-infraction zone,P<005. Compared with Sham operation group, the mRNA and protein expressions of CT-1, periostin, the contents of (O2-·), (OH·) and left heart weight index were increased in AMI + suvastatin group at non-infraction zone,P<005. Compared with AMI group, the mRNA and protein expressions of CT-1 and periostin were decreased in AMI + rosuvastatin group,P<005. Conclusion: Rosuvastatin may improve ventricular remodeling via inhibiting ROS production and CT-1, periostin expression in experimental rats after AMI.

With inlet temperature, specific gravity, feeding speed as independent variables, the comprehensive evaluating indexes of content of schisandrin and arctiin as dependent variable, the experimental data were fitted to a second order polynomial equation. Based on establishing the mathematical relationship between the comprehensive evaluating indexes and respective variables, Box-Benhnken central composite test and response surface analysis method was employed to optimize the spray drying technology of Biqiu granules ethanol extract. The optimal drying parameter was as follows: the inlet temperature was 175 degrees C, the specific gravity was 1.10, feeding speed was 32 r x min(-1). Under these conditions, the comprehensive evaluating indexes of spraying dry processes was 92.68, which was close to the model prediction. The spraying dry technology of Biqiu granules ethanol extract optimized by response surface methodology was accurate and feasible, which provided theoretical experiment basis for the industrialization production.
Chemistry, Pharmaceutical ; methods ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Ethanol

First with the qualified rate of granules as the evaluation index, significant influencing factors were firstly screened by Plackett-Burman design. Then, with the qualified rate and moisture content as the evaluation indexes, significant factors that affect one-step pelletization technology were further optimized by Box-Behnken design; experimental data were imitated by multiple regression and second-order polynomial equation; and response surface method was used for predictive analysis of optimal technology. The best conditions were as follows: inlet air temperature of 85 degrees C, sample introduction speed of 33 r x min(-1), density of concrete 1. 10. One-step pelletization technology of Biqiu granules by Plackett-Burman design and Box-Behnken response surface methodology was stable and feasible with good predictability, which provided reliable basis for the industrialized production of Biqiu granules.
Chemistry, Pharmaceutical ; methods ; Drugs, Chinese Herbal ; chemistry ; Temperature

Objective To investigate the relationship between estrogen receptor and cisplatin resistance in ovarian cancer SKOV3 cells.Methods A total of 30 mice were inoculated 4 × 106 ovarian cancer SKOV3 cells to establish the xenograft tumor model with human ovarian cancer SKOV3 cells.And they were randomly divided to control group and test group with 15 cases per group.Control group was given 0.9％ NaC1,intraperitoneal injection,once a week,a total of 5 times.Test group was given cisplatin 20 mg · kg-1,intraperitoneal injection,once a week,a total of 5 times.Then the ovarian cancer SKOV3 cells were collected from two groups.The expression of estrogen receptor in ovarian cancer SKOV3 cells was detected by immunocytochemistry and flow cytometry.MTT assay and flow cytometry were used to detect the effect of proliferation and apoptosis in ovarian cancer SKOV3 cells with different concentrations of cisplatin (0,1,5,10,15,20 μg · mL-1) combined with ICI182 (0,1,5,10,20 μmol · L-1) or alone.Results In control and test groups,the immune cell chemistry score (HIS score) were (10.04 ± 2.93),(6.73 ±2.37)points;the expression of estrogen receptor were 231.39 ± 15.33,205.81 ± 12.50 with significant difference(P ＞ 0.05).ICI182 had anti-proliferative effect on the control and test groups with dose-dependent and time-dependent manners.The inhibition rate of proliferation significantly increased by ICI182 combined with cisplatin (P ＜0.01).After treated with 10 μmol · L-1 ICI182 + 10 μg · mL-1 cisplatin in the test group and control group,the inhibition rates were (70.07-± 0.64) ％ and (82.13 ± 0.87) ％ with significant difference (P ＜ 0.01).Conclusion Estrogen receptor is involved in the process of drug resistance,and it is possible to reverse the cisplatin resistance of ovarian cancer cells by inhibiting estrogen receptor protein by ICI182.

This paper analyzes the scientific research guidance, author's area & units distribution, coauthor rate and quotations in "Chinese Journal of Medical Instrumentation", and offers some suggestions for the Journal so as to play an important role in scientific research in the future.
Bibliometrics ; China ; Humans ; Journalism, Medical ; Multivariate Analysis ; Periodicals as Topic ; statistics & numerical data

To investigate the effect of Tanreqing injection on immune activity of peripheral blood lymphocytes of patients with lung cancer. The peripheral blood lymphocytes of patients with lung cancer and healthy persons were separated by the density gradient centrifugation method for subsequent experiments, with those from healthy persons as the positive control. The effect of Tanreqing injection on stimulating the proliferation of lymphocytes with phytohemagglutinin (PHA) was determined by MTT method. The effect of Tanreqing injection on the lymphocyte secretions of IFN-γ and TNF-α and the subset ratio of lymphocytes cultured separately or with Tanreqing injection of different concentrations were examined by ELISA and flow cytometry (FCM) respectively. In addition, the LDH release assay was used to detect the cytotoxicity of cytotoxic T cells (CTL) and natural killer cells (NK). According to the findings, all of immunological indexes of lymphocytes from patients with lung cancer were weaker than that of healthy persons, but with the obvious increases in proliferation activity and IFN-γ and TNF-α secretions of lymphocytes co-cultured with Tanreqing Injection (P < 0.05). Among lymphocyte subsets co-cultured with Tanreqing Injection, CD3+, CD3+ CD4+ and CD3- CD16 + 56+ cell ratios notably increased, whereas CD4+ CD25+ Treg cell ratio obviously decreased (P < 0.05). In the meantime, Tanreqing injection can markedly promote the cytotoxicities of CTL and NK (P < 0.05). In conclusion, Tanreqing injection shows a significant effect in promoting the immune activity of lymphocytes from patients with lung cancer and their anti-tumor immunity.
Cell Proliferation ; drug effects ; Cells, Cultured ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Interferon-gamma ; genetics ; immunology ; Killer Cells, Natural ; drug effects ; immunology ; Lung Neoplasms ; drug therapy ; genetics ; immunology ; physiopathology ; T-Lymphocytes, Cytotoxic ; drug effects ; immunology ; Tumor Necrosis Factor-alpha ; genetics ; immunology

Objective To investigate the changes in the serum MMP-9 (matrix metalloproteinase-9) and the expressions of MMP-9 in lung, kidney and intestine in rats with multiple organ dysfunction syndrome (MODS) and confirm extracellular matrix injuries being the mechanism in MODS in order to propose a novel theoretical basis for cfinical treatment of MODS. Method Forty wister rats were randomly divided into two groups: control group (n=8) and MODS model group (n=32). The rats of model group were further divided into four subgroups ac-cordingto the time elapsed after modelling: 12 h (n=8), 24 h(n=8) ,48 h(n=8) and 72 h (n=8), and were modelled by celiac injection of mixed liquid of zymosan-paraffin (4 mL/100 g) after blood loss (1mL/100 g) by extirpating their left eyes. Blood,lung, kidney and intestine were sampled 12,24,48 and 72 hours after models were established. The histological changes in the lung, kidney and intestine of the rats were observed by light mi-croscope. The serum MMP-9 were measured by enzyme-linked immunosorbent assay (ELISA). The immunohisto-chemistry was used to observe the expression of MMP-9 in lung,kidney and intestine during different phases of MODS. The data were processed by one-way ANOVA and Bivariate analysis. Results Compared with control group, the organs were injured by congestion, edema and inflammatory cells infiltration to a certain extent in model groups. The serum MMP-9 increased markedly 12 hours after modelling (P<0.01 ) and peaked 48 hours later. The expressions of MMP-9 in lung, kidney and small intestine significantly increased from 12 h to 72 h after mod-elling (P<0.01 or 0.05). Conclusions The MMP-9 increased both in serum and tissue are closely associated with the pathological process of MODS. The mechanism of organ damage probably attributes to the damage of extra-celluar matrix and tissue construction.