Background and Objectives: Hepatic functions in patients with nodular goiter, who received long-term hormonal suppressive treatment was not well enlightened in publications. We studied liver specific enzymes activities in patients with nodular goiter after thyroidectomy. The aim of this study was comparative analysis of treatment outcomes of patients who were previously treated with L-thyroxine and were operated skipping hormonal treatment. Materials and Methods: 88 patients (basic group) with nodular goiter underwent surgical operation without any drug treatment. 86 patients in control group received hormonal suppressive treatment with L-thyroxine for 6-12 months and then underwent thyroidectomy. Liver ultrasound (US) scanning and laboratory tests were comparatively studied 3, 6, 12 months after thyroidectomy. Results: Patients that received long-term hormonal suppressive treatment and then admitted for surgery, suffered from discomfort in upper right abdominal quadrant. The ALT, AST, Alkaline Phosphates and GGT activity of those patients was increased, and steatohepatitis was identified in US investigations. Also, total bilirubin, especially the unconjugated fraction concentration was higher in control group. The hepatocytes functional activity in basic group was slightly affected. Conclusion Long-term hormone suppressive therapy resulted in dysfunction of hepatocytes. It’s highly recommended to the patients with hormonal therapy resistant nodules to undergo surgical treatment.