1.Association of Membrane Thickness, Histopathologic Findings and Premature Rupture of the Membranes.
Joon Cheol PARK ; Sung Do YOON
Korean Journal of Obstetrics and Gynecology 2003;46(7):1385-1390
OBJECTIVE: The aim of our study was to compare the thickness and histopathologic changes in the fetal membrane between premature rupture of membranes (PROM) and intact membrane after delivery. METHODS: In a prospective study involving 31 patients who were divided into 4 groups such as <37 weeks without PROM, <37 weeks with PROM, >or=37 weeks without PROM, and >or=37 weeks with PROM, we measured the thickness of membrane and studied the histopathologic findings in vitro by light microscopy of histological sections. RESULTS: The membrane thickness of <37 weeks with PROM group was thinner (35.9 micrometer) than that (42.3 micrometer) of <37 weeks without PROM group, but there was no statistical significance. The membrane thickness of >or=37 weeks with PROM and >or=37 weeks without PROM were similar (25.6 micrometer, 26.0 micrometer). But the membrane thickness of >or=37 weeks with/without PROM was significantly thinner (25.8 micrometer) compared with that (38.9 micrometer) of <37 weeks with/without PROM. The histopathologic features of PROM positive group was amnionitis with neutrophilic infiltration, focally or diffusely necrotic change of amniotic membrane, separation of amniotic membrane and degeneration of chorionic villi. CONCLUSION: The thickness of fetal membrane between PROM group and intact membrane group was not different but the thickness of fetal membrane between <37 weeks and >or=37 weeks was statistically significant. The histopathologic change of PROM positive group was prominent as amnionitis. Further evaluation will be needed about the relationship between membrane thickness and PROM.
Amnion
;
Chorioamnionitis
;
Chorionic Villi
;
Extraembryonic Membranes
;
Female
;
Humans
;
Membranes*
;
Microscopy
;
Neutrophils
;
Pregnancy
;
Prospective Studies
;
Rupture*
2.Clinical study of chorionic villi sampling(CVS).
Hong Joon KYE ; Yeon Woo PARK ; Soo Jai SHIN ; Sung Do KIM ; Jai Yeong AHN
Korean Journal of Obstetrics and Gynecology 1993;36(9):3405-3417
No abstract available.
Chorion*
;
Chorionic Villi*
3.Repair of an oroantral communication by a human amniotic membrane: a novel technique.
Subha LAKSHMI ; Siva BHARANI ; Kalhan AMBARDAR
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2015;41(4):194-197
The amniotic membrane is the innermost layer of fetal membrane and is attached to the chorion in the placenta. This membrane has been used for nearly a century in varied fields such as ophthalmology, reconstructive surgery, and burn treatment. In this case report, we used a human amniotic membrane to repair an iatrogenic oroantral communication that occurred during the extraction of the patient's right upper second molar. A splint was given after the perforation was covered with human amniotic membrane and healing was clinically evaluated at various intervals. The outcome of the study revealed that the human amniotic membrane was an efficient graft material for repairing the defect caused by an iatrogenic oroantral communication following tooth extraction.
Amnion*
;
Burns
;
Chorion
;
Extraembryonic Membranes
;
Humans*
;
Membranes
;
Molar
;
Ophthalmology
;
Patient Rights
;
Placenta
;
Splints
;
Tooth Extraction
;
Transplants
4.Telomerase Activity and Expression of MIB-1 and bcl-2 in Human Chorionic Villi from Early and Term Normal Pregnancy.
Jung Sook CHO ; Young Soon KANG ; In Gul MOON ; Bum Chae CHOI ; Jong Pyo LEE ; Hoon Taek LEE ; Sung Ran HONG
Korean Journal of Pathology 2000;34(11):927-933
Telomerase is an enzyme that maintains telomeres and prevents telomere shortening, and may be linked with cellular proliferation or the aging process. The purpose was to examine telomerase activity in human chorionic villi from early and term normal pregnancies, and to analyze the correlation of telomerase activity (TA) with MIB-1 & bcl-2. A total of 37 placentae were obtained from 16 early and 21 term pregnancies. TA was assayed by telomeric repeat amplification protocol, and immunohistochemical staining was performed for MIB-1 & bcl-2 expression. TA & MIB-1 expression were strong in early placenta, but bcl-2 was highly expressed in term placentae. Thirteen (81.25%) of 16 early placentae showed TA, but only 2 (9.52%) of 21 term placentae expressed TA (p<0.01). MIB-1 was observed in nuclei of cytotrophoblast, and the expression rate was 16.09% in early placentae and 2.87% in term placentae (p<0.01). bcl-2 was observed only in the cytoplasm of syncytiotrophoblast. Term placenta demonstrated stronger expression of bcl-2 compared to early placentae (p<0.05). These findings suggest that TA, MIB-1 & bcl-2 expression are critically regulated over the course of gestation: cytotrophoblast, main cells of early chorionic villi, may be a common source of telomerase and proliferative activity. The TA showed good correlation with cellular proliferative activity. Syncytiotrophoblast, may be a main source of bcl-2 expression which is stronger in the term placentae.
Aging
;
Cell Proliferation
;
Chorion*
;
Chorionic Villi*
;
Cytoplasm
;
Humans*
;
Placenta
;
Pregnancy*
;
Telomerase*
;
Telomere
;
Telomere Shortening
;
Trophoblasts
5.In vitro development and chimeric efficiency of mouse-porcine interspecies chimeric embryos in different culture systems.
Ying WANG ; Jilong REN ; Yuran SONG ; Tang HAI ; Qi ZHOU ; Zhonghua LIU
Chinese Journal of Biotechnology 2016;32(7):975-985
With the advancements of stem cells and regenerative medicine, interspecies chimera has become a hot topic and will pave a new way of providing donor sources in organ transplantation. However, the interspecies chimera is confronted with a number of scientific questions and technical obstacles, including selections of appropriate embryonic stage and appropriate culture medium; those factors will deeply influence the developmental balance between donor cells and receptor embryos. Due to its relatively rapid reproductive cycle and similar organ size to human's, porcine is a very potential donor candidate to study these questions. To compare the development and chimeric efficiency of interspecies embryos, we tested and evaluated three different culture systems, PZM-3 (Porcine zygotic medium), culture medium for iPSCs (N2B27) and 3.5 h of N2B27 before PZM-3 (N2B27(3.5 h)), and two different embryonic stages, 8-cell and blastocyst in mouse-porcine chimeric embryos using parthenogenetically activated porcine embryos and mouse induced pluripotent stem cells (miPS). The results showed that, PZM-3 was beneficial for both development of chimeric embryos and miPSCs proliferation in porcine embryos in the 8-cell injection group. After early blastocyst injection, the chimeric efficiency did not appear significantly different among the three culture systems but was lower than 8-cell injection. In summary, the results suggest that 8-cell injection and PZM-3 culture medium are more beneficial to the in vitro development and chimeric efficiency of mouse-porcine chimeric embryos.
Animals
;
Blastocyst
;
Chimera
;
Culture Media
;
Embryo Culture Techniques
;
veterinary
;
Embryo, Mammalian
;
Embryonic Development
;
Induced Pluripotent Stem Cells
;
cytology
;
Mice
;
Swine
6.Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm.
Kuisheng LIU ; Xiaocui XU ; Dandan BAI ; Yanhe LI ; Yalin ZHANG ; Yanping JIA ; Mingyue GUO ; Xiaoxiao HAN ; Yingdong LIU ; Yifan SHENG ; Xiaochen KOU ; Yanhong ZHAO ; Jiqing YIN ; Sheng LIU ; Jiayu CHEN ; Hong WANG ; Yixuan WANG ; Wenqiang LIU ; Shaorong GAO
Protein & Cell 2023;14(4):262-278
Self-organized blastoids from extended pluripotent stem (EPS) cells possess enormous potential for investigating postimplantation embryo development and related diseases. However, the limited ability of postimplantation development of EPS-blastoids hinders its further application. In this study, single-cell transcriptomic analysis indicated that the "trophectoderm (TE)-like structure" of EPS-blastoids was primarily composed of primitive endoderm (PrE)-related cells instead of TE-related cells. We further identified PrE-like cells in EPS cell culture that contribute to the blastoid formation with TE-like structure. Inhibition of PrE cell differentiation by inhibiting MEK signaling or knockout of Gata6 in EPS cells markedly suppressed EPS-blastoid formation. Furthermore, we demonstrated that blastocyst-like structures reconstituted by combining the EPS-derived bilineage embryo-like structure (BLES) with either tetraploid embryos or tetraploid TE cells could implant normally and develop into live fetuses. In summary, our study reveals that TE improvement is critical for constructing a functional embryo using stem cells in vitro.
Pregnancy
;
Female
;
Animals
;
Mice
;
Tetraploidy
;
Blastocyst
;
Embryo, Mammalian
;
Cell Differentiation
;
Embryonic Development
7.Modeling human pregastrulation development by 3D culture of blastoids generated from primed-to-naïve transitioning intermediates.
Zhifen TU ; Yan BI ; Xuehao ZHU ; Wenqiang LIU ; Jindian HU ; Li WU ; Tengyan MAO ; Jianfeng ZHOU ; Hanwei WANG ; Hong WANG ; Shaorong GAO ; Yixuan WANG
Protein & Cell 2023;14(5):337-349
Human pluripotent stem cells provide an inexhaustible model to study human embryogenesis in vitro. Recent studies have provided diverse models to generate human blastoids by self-organization of different pluripotent stem cells or somatic reprogramming intermediates. However, whether blastoids can be generated from other cell types or whether they can recapitulate postimplantation development in vitro is unknown. Here, we develop a strategy to generate human blastoids from heterogeneous intermediates with epiblast, trophectoderm, and primitive endoderm signatures of the primed-to-naïve conversion process, which resemble natural blastocysts in morphological architecture, composition of cell lineages, transcriptome, and lineage differentiation potential. In addition, these blastoids reflect many features of human peri-implantation and pregastrulation development when further cultured in an in vitro 3D culture system. In summary, our study provides an alternative strategy to generate human blastoids and offers insights into human early embryogenesis by modeling peri- and postimplantation development in vitro.
Humans
;
Pluripotent Stem Cells/metabolism*
;
Embryo, Mammalian/metabolism*
;
Cell Differentiation
;
Blastocyst
;
Cell Lineage
;
Embryonic Development
8.Chorionic villus sampling : experience of the first 510 cases in Korea.
Young Ho YANG ; Mi Sun KIM ; Yong Won PARK ; Sei Kwang KIM ; Jae Sung CHO ; Mee Jeong JEONG ; Chan Ho SONG
Korean Journal of Obstetrics and Gynecology 1993;36(7):906-915
No abstract available.
Chorion*
;
Chorionic Villi Sampling*
;
Chorionic Villi*
;
Female
;
Korea*
;
Pregnancy
9.Three-color Fluorescene in Situ Hybridization (FISH) Using Chorionic Villi Sampling (CVS) Transport Media.
Korean Journal of Perinatology 1997;8(2):153-156
No abstract available.
Chorion*
;
Chorionic Villi Sampling*
;
Chorionic Villi*
;
Female
;
In Situ Hybridization*
;
Pregnancy
10.Maternal Cell Contamination in Chorionic Villus Sampling : An Analysis of Frequency in 1,059 Consecutive Cases.
Bo Hoon OH ; Jung Min LEE ; Gyung Hwa LEE ; Ae Young JUNG ; Moo Sik GWON
Korean Journal of Obstetrics and Gynecology 2000;43(8):1384-1388
No abstract available.
Chorion*
;
Chorionic Villi Sampling*
;
Chorionic Villi*
;
Female
;
Pregnancy