Chronic Periodontitis ; complications ; diagnosis ; diagnostic imaging ; therapy ; Dental Pulp Diseases ; complications ; diagnosis ; diagnostic imaging ; therapy ; Dental Scaling ; Female ; Humans ; Periodontal Debridement ; Periodontal Diseases ; complications ; diagnosis ; diagnostic imaging ; therapy ; Radiography ; Root Canal Therapy ; Root Planing ; Young Adult

OBJECTIVETo investigate the effects of serum from smoking individuals or non-smoking individuals with periodontitis on Porphyromonas gingivalis (Pg) internalizing KB cells, and the expression of matrix metalloproteinase(MMP)-1, MMP-9, tissue inhibitor of metalloproteinase-1 (TIMP-1) in the culture supernatant of KB cells.

METHODSThe venous blood of 20 periodontitis patients' (10 smoking and 10 non-smoking) was extracted under the informed consent and centrifuged for serum. The smoking-individual serum (Y group) and non-smoking-individual (N group) serum were added to the model of Pg internalizing KB cells for 12 hours, plated on brain-heart infusion (BHI) and incubated anaerobically at 37 °C for 5 days. The colony forming units (CFU) of cell-invasive bacteria were estimated by colony counting. MMP-1, MMP-9 and TIMP-1 protein levels in culture supernatant were determined by enzyme-linked immunosorbent assay(ELISA) in the two groups following co-culture of Pg with KB cells for 12 hours.

RESULTSThe CFU were (11.2 ± 1.1)×10(4), (12.6 ± 1.2)×10(4), (44.7 ± 1.3)×10(4) CFU/ml when adding 200, 400, 800 µl Y-group serum to the model of Pg co-culture with KB cells and when the serum was extracted from N group, the CFU were (33.6 ± 1.4)×10(4),(38.9 ± 1.1)×10(4), (11.2 ± 1.2)×10(4) CFU/ml respectively. When 200, 400, 800 µl Y group-serum was added to co-culture fluid of Pg internalizing KB cells, the concentrations of MMP-1 secreted from KB cells were (107.2 ± 21.5), (165.9 ± 20.2), (434.4 ± 48.0) µg/L respectively, the concentrations of MMP-9 were (3.99 ± 0.29), (4.21 ± 0.61), (5.62 ± 0.47) µg/L respectively, the concentrations of TIMP-1 were (401.3 ± 12.7), (418.3 ± 28.5), (637.3 ± 37.3) µg/L. When the serum (200, 400, 800 µl) extracted from N group, the concentration of MMP-1 and MMP-9 secreted by KB cell were (77.6 ± 10.8), (84.7 ± 10.2) and (98.2 ± 9.7) µg/L and (3.84 ± 0.52), (4.02 ± 0.68), (4.25 ± 0.37) µg/L, respectively. The concentration of TIMP-1 were (67.3 ± 26.9) , (89.4 ± 22.7) and (78.2 ± 16.5) µg/L secreted by KB cells in the course of Pg internalized KB cell. With the increasing of Y group-serum, the more MMP-1, MMP-9 and TIMP-1 were secreted by KB cells(P < 0.05). When 800 µl Y group-serum was added compared with N group-serum to the Pg co-culture with KB model, the more MMP-1, MMP-9 and TIMP-1 were secreted by KB cells(P < 0.05), when 400 µl Y group-serum was added compared with N group-serum to the Pg co-culture with KB model, the more MMP-1 and TIMP-1 were secreted by KB cells (P < 0.05).

CONCLUSIONSThe smoking-serum might enhance Pg internalizing KB cells and enhance the expression of MMP-1, MMP-9 and TIMP-1 secreted from KB cells. The local microenvironment of smoking individual may contribute to the recurrence and progression of chronic periodontitis.

Coculture Techniques ; Humans ; KB Cells ; Matrix Metalloproteinase 1 ; metabolism ; Matrix Metalloproteinase 9 ; metabolism ; Porphyromonas gingivalis ; enzymology ; RNA, Messenger ; Serum ; Smoking ; Tissue Inhibitor of Metalloproteinase-1 ; metabolism

OBJECTIVETo investigate the prediction effect of the self-reported periodontal health on moderate and severe chronic periodontitis and its feasibility.

METHODSA total of 330 subjects from five districts of Shenyang city attended the survey.Each subject answered questions about self-reported periodontal health and risk factors of periodontitis and was given a periodontal examination. The predictiveness was assessed by logistic regression modeling measuring receiver operator characteristic (ROC) curve and area under curve (AUC), sensitivity and specificity.

RESULTSSelf-reported periodontal health for the prediction of moderate and severe chronic periodontitis was moderately effective, with AUC of 0.837, sensitivity of 74.8%, specificity of 71.3%.Self-reported periodontal health combined with risk factors of periodontitis was highly effective in prediction of moderate and severe chronic periodontitis, with AUC of 0.912, sensitivity of 82.1%, specificity of 85.4%.

CONCLUSIONSSelf-reported periodontal health can be used for epidemiological investigation of moderate and severe chronic periodontitis.Self-reported periodontal health combined with risk factors of periodontitis can improve the accuracy of the results of the survey.

Chronic Periodontitis ; Data Collection ; Humans ; Logistic Models ; Periodontitis ; epidemiology ; ROC Curve ; Risk Factors ; Self Report ; Sensitivity and Specificity ; Smoking

OBJECTIVETo evaluate the degree and pattern of alveolar bone defect in aggressive periodontitis (AgP) using cone-beam CT (CBCT), and to investigate the distribution of alveolar bone defects in aggressive periodontitis.

METHODSForty AgP patients (age: 14-36 years, male: 15 cases, female: 25 cases) were selected by simple random method and scanned by CBCT. NNT software was applied to measure the average degree of alveolar bone defects and bone loss types in different regions.

RESULTSIn forty AgP patients, 86.6% (3,769/4,352) sites presented moderate and severe alveolar bone defects. In the maxilla, the molar areas presented the heaviest alveolar bone defect [(6.3±0.7) mm], the canine areas showed the lightest bone loss [(4.8±0.8) mm]. In the mandible, the incisal areas presented the heaviest alveolar bone defect [(5.9±0.9) mm], the canine areas showed the lightest bone loss[(5.1±0.7) mm]. The degree of alveolar bone defect in the areas of maxillary canine, maxillary molars, mandibular premolar was significantly different (P<0.05). The degree of alveolar bone defect in mandibular canine and mandibular molars was significantly differenct (P<0.01). The most serious alveolar bone defect was in the mesial side of maxillary molar [(6.9±0.7) mm] and the mesial side of mandibular incisor [(6.5±1.1) mm]. The oblique bone defects were found in the mesial part of the first molars in mandibula [13.6% (42/308)], the first molars in maxilla [12.0% (39/316)] and the first premolar in maxilla [10.8% (34/316)].

CONCLUSIONSThe alveolar bone defects of generalized AgP patients were serious. The most serious areas were located in the mesial side of maxillary molars and the mesial side of mandibular incisor.

Adolescent ; Adult ; Aggressive Periodontitis ; diagnostic imaging ; Alveolar Process ; diagnostic imaging ; Bicuspid ; Cone-Beam Computed Tomography ; Cuspid ; Female ; Humans ; Incisor ; Male ; Mandible ; diagnostic imaging ; Maxilla ; diagnostic imaging ; Molar ; Software