1.Recurrent double coronary artery-pulmonary fistulae one year after surgical ligation.
Jun-Sheng MU ; Jian-Qun ZHANG ; Fan ZHOU ; Hong-Xing NIU
Chinese Medical Journal 2013;126(22):4288-4288
Aged
;
Arterio-Arterial Fistula
;
pathology
;
surgery
;
Coronary Vessel Anomalies
;
pathology
;
surgery
;
Coronary Vessels
;
pathology
;
surgery
;
Humans
;
Male
2.Aortic valve replacement for quadricuspid aortic valve with regurgitation and stenosis in a renal transplant recipient.
Junsheng MU ; Xianshuai LI ; Jianqun ZHANG ; Ping BO ;
Chinese Medical Journal 2014;127(16):3033-3033
Aged
;
Aortic Valve
;
surgery
;
Cyclosporine
;
therapeutic use
;
Female
;
Heart Defects, Congenital
;
drug therapy
;
surgery
;
Heart Valve Diseases
;
drug therapy
;
surgery
;
Heart Valve Prosthesis
;
Humans
;
Immunosuppressive Agents
;
therapeutic use
;
Kidney Transplantation
;
Mycophenolic Acid
;
analogs & derivatives
;
therapeutic use
;
Transplant Recipients
3.Cardiac surgical operation after renal transplantation.
Junsheng MU ; Fan ZHOU ; Xianshuai LI ; Jianqun ZHANG ; Ping BO
Chinese Medical Journal 2014;127(10):1990-1991
4.Culture effects of mouse induced pluripotent stem cells by three-dimensional films in vitro.
Shijun XU ; Junsheng MU ; Email: WESLEYMU@HOTMAIL.COM. ; Jianqun ZHANG ; Ping BO
Chinese Journal of Cardiology 2015;43(12):1073-1077
OBJECTIVETo observe the proliferation and differentiation capacities of mouse induced pluripotent stem cells (miPSCs) cultured in 3-hydroxybutyrate-co-3-hydroxyhexanoate(PHBHHx) three-dimensional films three-dimension films for the purpose of finding a suitable polymeric biomaterials for forming myocardial patches.
METHODSmiPSCs were recovered, passaged, cultured and identified, then miPSCs divided into the experimental and control groups. MiPSCs in the experimental group were cultured with PHBHHx three-dimension films. MiPSCs in the control group were cultured with traditional culture dish. Stem cell culture medium or differential medium were added to miPSCs to detecte cell vitality by CCK-8 after 72 hours or to measure the cTnT expression of miPSCs through immunofluorescence or the cTnT expression quantity through flow cytometry after 15 days.
RESULTSCell activity assay showed that the absorbance values were 0.836 ± 0.038 in the experimental group, 0.312 ± 0.004 in the control group (P<0.05). Scanning electron microscope (SEM) observation showed that miPSCs grew well on the PHBHHx dimensional films with normal shape. Immunofluorescence results demonstrated positive cTnT expression in both groups and flow cytometry measured cTnT expression was (60.32 ± 1.76)% in the experimental group and (47.54 ± 1.46)% in the control group (P<0.05).
CONCLUSIONSmiPSCs can survive, proliferate and differentiate on PHBHHx dimensional films. miPSCs proliferation and differentiation capacities are significantly higher in PHBHHx three-dimensional films culture compared with the traditional cell culture.
3-Hydroxybutyric Acid ; Animals ; Biocompatible Materials ; Caproates ; Cell Culture Techniques ; Cell Differentiation ; Induced Pluripotent Stem Cells ; Mice
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