OBJECTIVETo compare acoustically evoked short latency negative responses (ASNR) elicited from normal and profound hearing loss guinea pig ears and to confirm their vestibular nuclei origination.

METHODSForty healthy guinea pigs were employed in the experiment, which were randomly divided into the control group (8 subjects, 16 ears), the masking group (16 subjects, 32 ears) and the deafened group (16 subjects, 32 ears). Air conductive white noise was chosen for masking. Masking dilemma was avoidable by an appropriate 15 dB gap between stimulus and masking sound. Both the masking group and the deafened group were further divided into ASNR group and non-ASNR group based on the presence of ASNR. Electrolytic lesion was conducted to the vestibular nuclei, followed by ABR/ASNR recording. The lesioned brainstem slices were microscopically verified.

RESULTSIn the masking group, ASNR were present in 24 ears (75.0%, 24/32) and 12 ears (46.2%, 12/26) in deafened group, showing statistically higher presence rate for masking group (χ(2)=5.07, P=0.024). There were no significant differences for the ASNR threshold and latency between the masking ASNR group and the deafened ASNR group. For the two ASNR groups, electrolytic destruction to the vestibular nuclei subsequently eliminated the ASNR. Brainstem slice proved the accurate sites of electrolytic lesion.

CONCLUSIONModerate white noise masking prevents hearing system potential overlap without affecting vestibular system, therefore, ASNR is successfully elicited in normal guinea pigs. Both ASNRs from normal and deafened guinea pigs are of similar natures and origination from, the vestibular nuclei.

Animals ; Deafness ; physiopathology ; Evoked Potentials, Auditory, Brain Stem ; Guinea Pigs ; Hearing ; physiology ; Hearing Tests ; Noise ; Perceptual Masking ; Reaction Time ; Sound ; Vestibular Nuclei ; physiology

OBJECTIVEThis study established a model of acoustically evoked short latency negative response (ASNR) in guinea pigs. Stereotaxic coordinate guided electrolytic lesion was applied to animal brainstem nuclei, the vestibular nucleus and the cochlear nucleus, to define the neural origin of ASNR.

METHODSTwenty four guinea pigs with normal hearing were randomly divided into the control group (8 subjects, 16 ears) and the deafened group (16 subjects, 32 ears). Each animal experienced the auditory brainstem response (ABR) test. According to the presence of ASNR, the deafened group was further divided into ASNR group and non-ASNR group. Electrolytic lesion was conducted to the vestibular nucleus and cochlear nucleus respectively, followed by ABR test. The lesion structures were verified by brainstem slice and microscope.

RESULTSIn deafened group, the ASNR was elicited in 10 ears (31.3%). The ASNR was eliminated due to the electrolytic destruction to the vestibular nucleus, but it remained unchanged after the same procedure to the cochlear nucleus.

CONCLUSIONIt is clear that the ASNR is originated from the vestibular nucleus, but not the cochlear nucleus.

Acoustics ; Animals ; Cochlear Nucleus ; Evoked Potentials, Auditory, Brain Stem ; Guinea Pigs ; Reaction Time ; Saccule and Utricle ; Vestibular Nuclei

OBJECTIVESSonotubometry is wildly used in developed countries, offers a easy, versatile and objective way to assess the function of Eustachian tube. The objectives of this study are to develop a low-cost digitalized device.

METHODSThe digitalized sonotubometry device was made of a personal computer with sound input and output accessories. The PC sound card, connected to a signal amplifier, exported probe band noise, while feedback sound was collected by a microphone. All 36 volunteers (72 ears) were involved in the sonotubometry test with JK-04A and CHN-08, in the conditions of dry-swallowing and wet-swallowing. The data were statistically analyzed using SPSS 13.0.

RESULTSThe basic electroacoustic performance of the CHN-08 was acceptable. CHN-08 (7 kHz) detection and JK-04A consistency of kappa index was 0.472; CHN-08 (8 kHz) and JK-04A consistency of kappa index was 0.487; CHN-08 (7 kHz) and CHN-08 (8 kHz) consistency of kappa index was 0.688.Under the JK-04A, CHN-08 (7 kHz) and CHN-08 (8 kHz) three setups, Eustachian tube opening frequency was significantly lower for wet-swallowing than dry-swallowing, P < 0.05.

CONCLUSIONSA low-cost digitalized sonotubometry is successfully developed. Its electroacoustic performance proved acceptable by authority assessments. The detection rate of CHN-08 is in accordance with that of JK-04A moderately. The study revealed that Eustachian tube is opened more easily by dry-swallowing than by wet-swallowing.

Acoustic Impedance Tests ; Audiometry ; instrumentation ; Equipment Design ; Eustachian Tube ; physiology ; physiopathology ; Humans ; Man-Machine Systems