1.Effects on blood cell numbers and cytokines of dermal application rocket kerosene in mice.
Bingxin XU ; Jianying WANG ; Zhiguo LIU ; Chenglin LI ; Heming YANG ; Xiaotong LOU ; Jianzhong LI ; Yan CUI ; Email: DRYANCUI@ALIYUN.COM.
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(9):679-681
OBJECTIVETo detect the number of cells and the level of IL-2, IL-4, IL-6, IL-10, TNF-alpha, IFN-γ and IL-17 cytokines in the peripheral blood of mice exposed to rocket kerosene by skin.
METHODICR mice were randomly divided into the normal control group and RK experimental group (400 µl×1 group). RK undiluted fuel were applied directly to the dorsal skin of the mice. In control groups were treated with sesame oil (SO). the number of blood cells were detected by automatic blood cell counter and the level of IL-2, IL-4, IL-6, IL-10, TNF-alpha, IFN-γ and IL-17 cytokines in serum were detected by using flow cytometry and BD CBA Flex set kit.
RESULTCompared with the normal group, WBC and LYM had a decreasing tendency 2 h and decreased significantly 6 h, 12 h and 1 d after RK exposure (P<0.05). They increased significantly 7 d after RK exposure (P<0.05). Compared with the normal group, the level of IL-6 increased significantly 2 h, 6 h, 12 h,1 d and 3 d (P<0.05). The level of TNF-α increased significantly 2h, 3d, 5d and 7d (P<0.05). The level of IL-10 increased significantly 2 h, 6 h, 3 d, 5 d and 7 d (P<0.05). The level of IFN-γ increased significantly 6 h and 3 d (P< 0.05). The level of IL-17 significantly increased 3 d, 5 d and 7d (P<0.05).
CONCLUSIONRK can change the number of immune cells, causing the immune cytokine changes in mice after RK cutaneous exposure.
Administration, Cutaneous ; Animals ; Blood Cell Count ; Cytokines ; blood ; Flow Cytometry ; Kerosene ; toxicity ; Mice ; Mice, Inbred ICR
Result Analysis
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