OBJECTIVETo study the HIV-1 genotypes and transmitted drug resistance (TDR) in Dehong prefecture of Yunnan province in 2012.

METHODSReferring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS), 60 plasma samples of recently reported HIV-infected individuals between 16 and 25 years old were collected in Dehong prefecture from January to August 2012. Genotyping of partial pol gene was performed by using reverse transcriptional PCR. HIV-1 genotype and the prevalent levels of HIV-1 drug resistance transmission were analyzed.

RESULTS52 plasma samples were successfully sequenced and analyzed. Among them, 59.6% were Chinese, and the rest (40.4%) were Burmese. Based on pol sequences, identified HIV genotypes would include unique recombinant forms (URFs, 38.5%), subtype C (34.6%), CRF01_AE (21.2%), CRF08_BC (3.8%), and subtype B (1.9%). One drug resistant mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) was detected in respective two sequences. Based on the statistical method of HIVDR-TS, the prevalence of transmitted HIV-1 drug resistance was adjusted as a moderate level (5%-15%).

CONCLUSIONDiverse HIV-1 genotypes were found in this study, and the current HIV-1 drug resistant strains transmission was catalogued as moderate prevalence level in Dehong.

Adolescent ; Adult ; China ; epidemiology ; Drug Resistance, Viral ; genetics ; Female ; Genotype ; HIV Infections ; epidemiology ; virology ; HIV-1 ; drug effects ; genetics ; Humans ; Male ; Young Adult

OBJECTIVETo obtain information on HIV disease progression through western blot (WB) band profile.

METHODS1466 available serum samples of newly reported HIV/AIDS cases, confirmed by WB from Dehong prefecture of Yunnan province in 2011, were tested, using the BED HIV Incidence Capture Enzyme Immunoassay (BED-CEIA) and Limiting Antigen Avidity Enzyme Immunoassay (LAg-Avidity EIA), respectively. Differences of WB band profiled between recent infections and established infections were then compared.

RESULTSInfections defined as 'recent' showed lower mean antibody intensities to p17, p24, p31, gp41, p51, p55, p66, gp120 and gp160 in WB when compared to those established infections with statistical difference(P < 0.05).

CONCLUSIONThese WB band profiles which were considered as 'recent' infections would include factors as:lack of antibody reactivity to gp41 antigen, with intensity scores of all bands lower than 13, and those intensity scores of antibody to env protein lower than 5 or to pol protein lower than 4.

Acquired Immunodeficiency Syndrome ; blood ; diagnosis ; epidemiology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Blotting, Western ; Child ; Child, Preschool ; China ; Female ; HIV Seropositivity ; HIV-1 ; immunology ; Humans ; Male ; Middle Aged

OBJECTIVETo study the HIV-1 genotypes and transmitted drug resistance (TDR) in Dehong prefecture of Yunnan province in 2013.

METHODSReferring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS), 54 plasma samples of recently reported HIV-infected individuals, aged between 16 and 25 years, were collected in Dehong prefecture from January to August 2013. Genotyping of partial pol gene was performed by using reverse transcriptional PCR. HIV-1 genotype. Prevalent levels of HIV-1 drug resistance transmission were analyzed.

RESULTSForty-eight plasma samples were successfully sequenced and analyzed. Among them, 45.8% were Chinese and the rest 54.2% were all Burmese. Based on pol sequences, identified HIV genotypes included subtype C (41.7%), URF (31.3%), CRF01_AE (12.5%), CRF07_BC (10.4%), CRF08_BC (2.1%) and subtype B (2.1%), C subtype appeared dominated in Chinese while URF was dominated in Burmese. One drug resistant mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) was detected in one sequence from Burmese. Based on the statistical method of HIVDR-TS, the prevalence of transmitted HIV-1 drug resistance was adjusted as < 5%.

CONCLUSIONDiverse HIV-1 genotypes were found in this study, and the current HIV-1 drug resistant strains transmission was catalogued as at low prevalence level, in Dehong. To prevent the increase of the prevalence of transmitted HIV-1 drug resistance, standard treatment and scientific management for people living with HIV/AIDS should be strictly followed. Meanwhile, relevant surveillance, including drug resistance surveillance should also be performed among cross-border migrant population.

Anti-HIV Agents ; pharmacology ; therapeutic use ; China ; Drug Resistance, Viral ; genetics ; Genes, pol ; Genotype ; HIV Infections ; drug therapy ; virology ; HIV-1 ; drug effects ; genetics ; Humans ; Mutation ; Polymerase Chain Reaction ; Prevalence ; Reverse Transcriptase Inhibitors