1.Age-related expression of plasma membrane Ca(2+) -ATPase isoform 2 in the cochleas of C57BL/6J mice.
Yan GAO ; Yanling TAO ; Email: 18907183041@163.COM. ; Hanqi CHU ; Jin CHEN ; Qingguo CHEN ; Liangqiang ZHOU ; Yun LIU ; Yang YU ; Yonghua CUI
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2015;50(11):934-938
OBJECTIVETo investigate the location and distribution of plasma membrane Ca²⁺ -ATPase isoform 2(PMCA2) in the cochleas of C57BL/6J mice at various ages (4w, 14w, 22w, 45w), and to reveal the relationship of PMCA2 and age-related hearing loss (AHL).
METHODSThe distribution of PMCA2 in the cochleas of C57BL/6J mice was detected by immunohistochemistry at various ages (4w, 14w, 22w, 45w). Real-time polymerase chain reaction (Rt-PCR) was used to detect the level of PMCA2 mRNA in the cochleas of C57BL/6J mice at the ages of 4, 14, 22 and 45 weeks old respectively. Using SPSS17.0 software for statistical analysis.
RESULTSPMCA2 was mainly located in the hear cells, stria vascularis, and spiral ganglion cells. Faint labeling of PMCA2 was also observed in spiral ligament. Hair cells missed and the number of spiral ganglion cells reduced with age. Expression of PMCA2 in the cochleas of C57BL/6J mice also showed age-related decreasing. The results of Rt-PCR demonstrated the expression of mRNA of gene (Atp2b2) at 14 weeks age was significantly less than 4 week-old mice cochlears (P<0.05). The expression of mRNA of gene (Atp2b2) at 22 weeks age was significantly less than 14 week-old mice cochlears (P<0.05). The expression of mRNA of gene (Atp2b2) at 45 weeks age was significantly less than 14 week-old mice cochlears (P<0.01).
CONCLUSIONSPMCA2 is mainly located in the hear cells, stria vascularis, and spiral ganglion cells. Faint labeling of PMCA2 is also observed in spiral ligament. The expression of PMCA2 demonstrates an age-related decrease with age. The mRNA expression level of PMCA2 gene(Atp2b2) in the cochleas of C57BL/6J mice displayed an age-related decrease. PMCA2 transporters may play a critical role in maintaining the normal morphology of the inner ear and it may be related to AHL.
Aging ; Animals ; Cochlea ; enzymology ; Hair Cells, Auditory ; metabolism ; Isoenzymes ; metabolism ; Mice ; Mice, Inbred C57BL ; Plasma Membrane Calcium-Transporting ATPases ; metabolism ; RNA, Messenger ; metabolism ; Real-Time Polymerase Chain Reaction ; Spiral Ganglion ; cytology ; metabolism ; Stria Vascularis ; cytology ; metabolism
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