Background: Stenotrophomonas maltophilia is a non-fermentative gram-negativebacillus which is widely recognised as an important nosocomial pathogen causing pneumonia,blood-stream, wound and urinary tract infections, particularly in immunosuppressed patients. Theaim of this study was to evaluate a nosocomial outbreak of by S. maltophilia in an intensive careunit of a tertiary hospital and evaluate unexpected multiclonality.Methods: A total of 11 isolates from respiratory cultures in intensive care unit of a 24 bedtertiary hospital obtained over a one months period and one isolate obtained from the nebuliserduring environmental screening were investigated. The bacteria were identified by Phoenix 100system. The clonal relatedness was evaluated by PFGE and semi-automated repetitive sequencebasedPCR. Genotyping tests were repeated for 10 serial subcultures.Results: PFGE and DiversiLab yielded 10 genotypic profiles for 12 isolates. Four to eightdifferent genotypes were observed from 10 subcultures of the same isolate.Conclusion: We conclude that, high genetic diversity and supposed multiclonalappearance of the outbreak isolates may be due to changing profiles during subcultures mostprobably depending on hypermutation.

Myxoinflammatory fibroblastic sarcoma (MIFS) is a rare low-grade, malignant soft tissue tumor that is usually observed in the extremities of adult patients. Magnetic resonance imaging findings for this tumor type have rarely been reported. We report a case involving the distal left femur of a middle-aged man and tumoral invasion of the bone, which, to our knowledge, has been previously described only once. He was treated with distal femoral tumor resection and reconstruction with a modular prosthesis. Histopathologic diagnosis confirmed MIFS. We reviewed literature of the diagnostic imaging and bone invasion findings associated with this tumor type.
Femoral Neoplasms ; pathology ; Fibrosarcoma ; pathology ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged