3.Detection of tubular proteinuria using gradient gel SDS-PAGE.
Korean Journal of Clinical Pathology 1991;11(3):529-536
No abstract available.
Electrophoresis, Polyacrylamide Gel*
;
Proteinuria*
4.Macro creatine kinase, type 2 on electrophoresis.
Hyub Youbg CHI ; Hee Sun JEON ; Young Joo CHA ; Hyoun Tae KIM ; Ae Ja PARK
Korean Journal of Clinical Pathology 1991;11(1):19-22
No abstract available.
Creatine Kinase*
;
Electrophoresis*
5.Features of hemoglobin in stieng minority in Binh Duong province
Journal of Vietnamese Medicine 1999;233(2):14-16
On studying 147 hemoglobin electrophoresis samples of the Stieng community, we refound that hemoglobin varieties distributed as following: - HbE: 54.45% including HbE homozygous: 6.12%; HbAe: 41.5% and -thal/HbE: 6.8%.- -thal: only -thal/HbE (-thal/HbE: 4.08%, -thal/HbE, 2.72%). -Normal cases: 45.58%. These results shown that HbE ratio is very height in Stieng community, it is necessary to have the special investigations to find out the causes
Hemoglobins
;
Minority Groups
;
Electrophoresis
6.Dosage of vitamines B1, B2, B6, PP in the injectable product Becozyme by capillary electrophoresis method
Pharmaceutical Journal 2005;0(5):20-22
A capillary electrophoresis method was used for simultaneous determination of vitamines: B1, B2, B6 and PP in multivitamines injection (Becozym-Roche). The method was simple, rapid, economic, accurate and in high precision. Relative errors were from 0.65% to 1.53% and recoveries were from 99.7% to 101.4%. The method can be applied in analysis other multi-component product to replace high performance liquid chromatorgaphy method
Electrophoresis
;
Methods
;
Vitamins
7.Dosage of ketoprofen in pharmaceutical products by local capillar electrophoresis
Pharmaceutical Journal 2003;331(11):21-23
Capillary Zone Electrophoresis (CZE) was used to quantify ketoprofen in 50mg ketoprofen capsules and ketoprofen gel 2.5%. The results were compared to results of spectrophotometric UV-VIS method. Results: the accuracies of 2 methods were different but not significant. Results of CZE method were rather lower than spectrophotometric UV-VIS method because impurities were separated in electrophoresis process. The authors concluded that CZE could be used to quantify ketoprofen in products
Ketoprofen
;
Electrophoresis
;
Adjuvants, Pharmaceutic
8.Community structures of total bacterial DNA, cultivable bacteria and prototrophs in bulk soil and rhizospheres
Neelawan Pongsilp ; Pongrawee Nimnoi ; Saisamorn Lumyong
Malaysian Journal of Microbiology 2016;12(1):1-14
Aims: It has been hypothesized that root exudates can be a nutritional factor influencing the bacterial community
structure as well as the occurrence of prototrophs and auxotrophs in rhizospheres. The present study was performed to
examine the community structures of total bacterial DNA, cultivable bacteria and prototrophs in 3 soil samples with
different levels of abundance of root exudates.
Methodology and results: Denaturing gradient gel electrophoresis (DGGE) was performed to examine the community
structures of total bacterial DNA, cultivable bacteria and prototrophs in 3 soil samples including bulk soil, rhizosphere of
a single plant species and rhizosphere of multiple plant species. For clustering analysis, a dendrogram generated from
the DGGE patterns revealed the different bacterial community structures in these soil samples. Both rhizospheres
claded together, separating from bulk soil. The DGGE patterns of cultivable bacteria showed particular fingerprints
corresponding to kinds of media and soil samples. Nutrient agar (NA) medium, isolation medium for prototroph (IMP)
and IMP supplemented with soil extracts were used for bacterial cultivations. Prototrophs were isolated and examined by
random amplified polymorphic DNA (RAPD) and 16S rRNA gene sequence analysis. The genetic diversity of
prototrophs in 3 soil samples was similar (approximately 5% to 10% similarities) and most of them (13 of 28 strains)
were members of Pseudomonas with 97% to 100% identities.
Conclusion, significance, and impact of study: The present study provides a strong evidence of the influence of root
exudates and plant species on bacterial community structures.
Denaturing Gradient Gel Electrophoresis
9.Serum Lipid Analysis Using Biphasic Electrophoresis.
Ji Young PARK ; Hyun Woo CHOI ; Jin So LEE ; Duck CHO ; Myung Geun SHIN ; Jong Hee SHIN ; Soon Pal SUH ; Dong Wook RYANG
Journal of Laboratory Medicine and Quality Assurance 2008;30(1):201-207
BACKGROUND: Recently biphasic agarose gel electrophoresis method using Chol/Trig ComboTM for simultaneous detection of cholesterol and triglyceride on lipoprotein fractions has been developed to facilitate the classification and interpretation of abnormal lipoprotein patterns of patients with hyperlipidemia. The author analysed serum lipid profiles using Chol/Trig ComboTM in the patients of OPD in Department of Internal Medicine. METHODS: Measurement of serum cholesterol and triglyceride using enzymatic method was performed in the sera of 415 patients from April, 2006 to July, 2006. Simultaneously, we electrophoresed serum cholesterol and triglyceride using Chol/Trig ComboTM with analysis software (ED BANK, Helena Laboratories, Saitama, Japan). RESULTS: According to ATP III guideline, we set up standard cholesterol as 200 mg/dL and triglyceride as 150 mg/dL, respectively, and the patients were classified into control, hypercholesterolemic, hypertriglyceridemic and hypercholesterolemic/hypertriglyceridemic groups, respectively. Hypercholesterolemic group had higher HDL and LDL, hypertriglyceridemic group had higher HDL, VLDL and the rates of positive modified LDL, hypercholesterolemic/hypertriglyceridemic group have higher VLDL and the rates of positive modified LDL than the control. Otherwise, the rates of positive modified LDL were 19.8% in the control, 23.6% in the hypercholesterolemic group, 50.9% in hypertriglyceridemic group, and 39.3% in hypercholesterolemic/hypertriglyceridemic group. CONCLUSIONS: The frequency of the rates of positive modified LDL and VLDL was higher in the hypertriglyceridemic group than those in the control.
Adenosine Triphosphate
;
Cholesterol
;
Electrophoresis
;
Electrophoresis, Agar Gel
;
Humans
;
Hyperlipidemias
;
Lipoproteins
10.Serum Lipid Analysis Using Biphasic Electrophoresis.
Ji Young PARK ; Hyun Woo CHOI ; Jin So LEE ; Duck CHO ; Myung Geun SHIN ; Jong Hee SHIN ; Soon Pal SUH ; Dong Wook RYANG
Journal of Laboratory Medicine and Quality Assurance 2008;30(1):201-207
BACKGROUND: Recently biphasic agarose gel electrophoresis method using Chol/Trig ComboTM for simultaneous detection of cholesterol and triglyceride on lipoprotein fractions has been developed to facilitate the classification and interpretation of abnormal lipoprotein patterns of patients with hyperlipidemia. The author analysed serum lipid profiles using Chol/Trig ComboTM in the patients of OPD in Department of Internal Medicine. METHODS: Measurement of serum cholesterol and triglyceride using enzymatic method was performed in the sera of 415 patients from April, 2006 to July, 2006. Simultaneously, we electrophoresed serum cholesterol and triglyceride using Chol/Trig ComboTM with analysis software (ED BANK, Helena Laboratories, Saitama, Japan). RESULTS: According to ATP III guideline, we set up standard cholesterol as 200 mg/dL and triglyceride as 150 mg/dL, respectively, and the patients were classified into control, hypercholesterolemic, hypertriglyceridemic and hypercholesterolemic/hypertriglyceridemic groups, respectively. Hypercholesterolemic group had higher HDL and LDL, hypertriglyceridemic group had higher HDL, VLDL and the rates of positive modified LDL, hypercholesterolemic/hypertriglyceridemic group have higher VLDL and the rates of positive modified LDL than the control. Otherwise, the rates of positive modified LDL were 19.8% in the control, 23.6% in the hypercholesterolemic group, 50.9% in hypertriglyceridemic group, and 39.3% in hypercholesterolemic/hypertriglyceridemic group. CONCLUSIONS: The frequency of the rates of positive modified LDL and VLDL was higher in the hypertriglyceridemic group than those in the control.
Adenosine Triphosphate
;
Cholesterol
;
Electrophoresis
;
Electrophoresis, Agar Gel
;
Humans
;
Hyperlipidemias
;
Lipoproteins