BACKGROUND: Recent blood gas analyzers offer electrolytes, Hb-derivatives (O2 Hb, HHb, COHb, MetHb and SulfHb), content of total hemoglobin (ctHb), and metabolites (glucose and lactate) as well as conventional blood gas parameters. Evaluation and efficient control of these instruments affect greatly monitoring and therapy of patients. The authors evaluated a recently introduced blood gas analyzer, the Roche OMNI 9 (Roche Diagnostics) for precision, analytic speed, function, features and comparability with other instruments according to NCCLS guidelines to see if it was suitable for the stat analyzer. METHODS: Commercial control materials, AUTO-trol PLUS Level 1, 2, 3 (Roche Diagnostics) were analyzed to evaluate the within-day precision, the between-day precision, and patient samples were used to evaluate comparability with other instruments. RESULTS: The within-day and the between-day precisions of the Roche OMNI 9 showed a very low CV of 0- 3.93% and 0- 3.99%, respectively, in all parameters except the lactate, which had moderate CV values of 7.74% and 5.93% in Level 3 QC material (low concentration). In compari-son with the Bayer instrument, correlation was high in all parameters with the r(2) value ranging from 0.9441 to 0.9901 except for COHb (r(2)=0.4239) and MetHb (r(2)=0.0034) whose concentrations were in the lower level. Glucose (r(2)=0.991), lactate (r(2)=0.9824), and hemoglobin (r(2)=0.9961) showed a rather high correlation. The Roche OMNI 9 could analyze 40 samples of blood gas and 27 samples of full parameter per hour, which was comparable to the Bayer M855. CONCLUSIONS: The Roche OMNI 9 showed high precision and rapid turnaround time for blood gas and in a selection of glucose, lactate and hemoglobin.
Blood Gas Analysis ; Electrolytes ; Glucose ; Humans ; Lactic Acid

BACKGROUND: Recently, there are so many newly developed equipments to detect blood gas analysis with serum electrolytes. And then, so many important parameters are quickly reported from laboratory department. So, many clinicians use the data to manage the critical patient. We attempted to detemine the correlation coefficient and adjustment equation. METHODS: Total 68 patients results of Na, K that are cheked using arterial whole blood(288 blood gas system) and venous serum electrolytes analyzer(NOVA 6) were evaluated and we detemined the coefficient of two methods from April to September, 1995. RESULTS: The correlations between the results obtained with NOVA 6 and that of 288 blood gas system were excellent for both Sodium and potassium(r>0.95, p<0.001 in both). In this study, we make the regression equation to adjust the differences between the two methods. Na : y=1.004190X+7.656643(r=0.9637, p<0.001) y=venous serum Na, x=arterial whole blood Na K: y=0.994831X+0.489631(r=0.9545, p<0.001) y=venous serum K, x=arterial whole blood K. CONCLUSIONS: Specimen(whole blood, serum), kind and content of heparin also affect the results of electrolytes. And then, the report of the result after adjustment of them using above equation will provide reinforcement of the efficacy of the results using arterial whole blood specmen. We recommend it especially for emergency laboratories for critical test.
Blood Gas Analysis ; Electrolytes* ; Emergencies ; Heparin ; Humans ; Sodium

PURPOSE: The results of arterial blood gas analysis using conventional liquid sodium heparin syringes are inaccurate due to the dilution effect, chelation of heparin and the electrolyte, and interference of the heparin electrolyte measurement. This study compared the accuracy of using heparin with a liquid sodium heparin syringe (LHs) and balanced lithium/zinc heparin syringe (BHs). METHODS: This study evaluated 6,778 cases who underwent an arterial blood gas test, serum electrolytes test, and complete blood count test among patients aged 18 years or older who visited the emergency room from November 1, 2016 to March 3, 2017. Finally, there were 2,383 cases using LHs and 2,584 cases using BHs. The results were compared between the groups using the LHs and BHs for sodium, potassium, and hemoglobin, and the agreement was compared using the Bland-Altman plot. RESULTS: Sodium difference value was the 5.714±5.696 mmol/L in the LHs group, -1.549±3.339 mmol/L in the BHs group. The potassium difference value was -0.650±0.494 mmol/L (LHs group) and -0.257±0.367 mmol/L (BHs group). The hemoglobin difference values were -0.556±1.116 g/dL (LHs group) and -0.170±1.062 g/dL (BHs group). The results showed that the BHs group was improved compared to the LHs group (p<0.001). CONCLUSION: The results of arterial blood gas analysis of sodium, potassium, and hemoglobin were more accurate for the BHs group than the LHs group based on the serum electrolytes and complete blood counts.
Blood Cell Count ; Blood Gas Analysis* ; Electrolytes* ; Emergency Service, Hospital ; Heparin* ; Humans ; Potassium ; Sodium ; Syringes*

Arterial blood gas analysis is essential laboratory test in monitoring of acid-base balance and respiratory care, but it has been known that there was many variabilities according to method of sampling and care before analysis. We compared the effects of liquid Na heparin and dry Li-heparin on blood gas analysis, hemoglobin and electrolytes, and studied in 25 patients who were taken elective operation with radial arterial eannulation. The samples were obtained from radial artery; sample 1, drawn to a total volume of 1 cc with 2cc plastic syringe utilizing liquid Na-heparin; sample 2, drawn to a total volume of 2 cc with 2 cc plastic syringe utilizing liquid Na-heparin ; sample 3, drawn to a total volume of 1 cc with Bard parker Preset 1 cc kit that pre-filled dry Li-heparin; control value, sampled arterial blood without heparin for measurement of hemoglobin and electrolytes. These samples were analyzed with minimum error, as possible. We obtained the following results. 1) There was no atatistical difference in pH, PCO2, PO2 and hemoglobin in each sample. 2) The value of Na+ and K+ were no statistical differences in sample 1,2 and 3, but sig- nificantly lower level than control value (P<0.01). 3) The Ca2+ level was significantly lower in sample 1 and 2(P<0.01) and not significantly different in sample 3 when compared with the control value. The Ca level was significantly higher in sample 2 and 3 when compared with sample 1 (P<0.01). So we conclude that arterial blood gas analysis and hemoglobin level showed no statistical difference between the liquid Na heparin and the dry Li-heparin. Heparin is not suitable for Na+ and K+ measurement. Low dose liquid Na heparin and dry Li heparin is reliable for obtaining Ca2+ level.
Acid-Base Equilibrium ; Blood Gas Analysis* ; Electrolytes ; Heparin ; Humans ; Hydrogen-Ion Concentration ; Plastics ; Radial Artery ; Syringes

This experiment was undertaken to study the effects of different ratios of N2O-O2 in N2O- halothane anesthetized rabbits weighing about 4-4.5 Kg. The N2O-O2 ratios were 2:1 (group 1), 3:1 (group 2) and 4:1 (group 3). We measured the mean arterial pressure, intracranial pressure and cerebral pressure and analysed the blood gas and electrolytes in each group and compared each group to the other two. The results are summarized as follow; The mean arterial pressure was 101.7+/-14.6, 92.7+/-11.0 and 89.4+/-15.1mmHg in each group respectively. The mean arterial pressure of the group 3 decreased more significantly than that of group 1. The intracranial pressure was 12.7+/-4.3, 11.8+/-4.2 and 13.6+/-4.6mmHg in each group respectively and was non-significant as compared to other groups. The cerebral perfusion pressure was 90.8+/-13.3, 82.6+/-9.9 and 76.9+/-16.9mmHg in each group respectively and the cerebral perfusion pressure of group 3 decreased more significantly than that of group l. In the blood gas analysis, PaQ2 was 162+/-27, 119+/-13 and 105+/-8 mmHg in each group respectively and was non-significant in respect to other groups. PaCO2 and O2 content were not different significantly in each group and were within normal values. The oxygen saturation was 98.9+/-0.3, 97.8+/-0.4 and 97.6+/-1.1% in each group respectively and group 2 and 3 decreased more significantly than group 1. Na+, Cl-, HCO-(3), pH and BE were not different significantly in each group, pH, BE and HCO-(3) were measured as being slightly low values, but PaCO2 was kept within normal values. These are explained as probably being due to metabolic acidosis. The serum K' was 2.94+/-0.46, 3.07+/-0.44 and 3.40+/-0.7 mEq/1 in each group respectively and the serum K+ of group 3 increased more significantly than groups 1 and 2. With these results, it is suggested that a ratio above a 2:1 ratio of N2O-O2 is more dangerous in halothane-N2O anesthesia.
Acidosis ; Anesthesia* ; Arterial Pressure* ; Blood Gas Analysis ; Electrolytes* ; Halothane* ; Hydrogen-Ion Concentration ; Intracranial Pressure* ; Oxygen ; Perfusion ; Rabbits* ; Reference Values

BACKGROUND: Changes in acid-base balance and serum electrolytes by infusion of lactated Ringer's solution in liver cirrhosis patienst during liver surgery are poorly characterized. In this study, we evaluated the effects of infusing large amount of lactated Ringer's solution on acid-base and serum electrolytes during liver surgery in cirrhosis patients. METHODS: Thirty-two patients were divided into two groups. Group I (n = 21) was made up of patients who had received liver lobectomy without cirrhrosis. Group II (n = 11) was made up of patients who had received liver lobectomy with cirrhosis above a moderate level. Arterial blood gas and serum electrolyte levels were checked 4 times during the study in each patient: just after the operation start, after infusing 3,000 ml and 6,000 ml of lactated Ringer's solution during operation, and 30 minutes after arrival at the postanesthesia care unit. RESULTS: pH and base excess decreased according to the amount of lactated Ringer's solution used in both groups and these results were significant. Serum electrolyte levels were not changed and only Ca2+ levels were significantly different in the two groups. The cause of changing of Ca2+ levels found out by intravenous infusion of Ca2+ solution. CONCLUSIONS: In liver surgery patients with or without liver cirrhosis decreased pH and base excess in serum by increased amount of used lactated Ringers solution during liver surgery but in serum electrolytes and others acid-base parameters, CVP, changes on there were not any statistical significant. When a large amount of LR solution is used in liver surgery, we recommend regular arterial blood gas analyses for acid-base balance and an infusing speed of 20 ml/kg/h.
Acid-Base Equilibrium ; Blood Gas Analysis ; Electrolytes ; Fibrosis* ; Humans ; Hydrogen-Ion Concentration ; Infusions, Intravenous ; Liver Cirrhosis ; Liver*

The determination of chemical purity of andrographolide by coulometric titration method is studied in this paper. The coulometric titration was carried out in a mixture composed of 4 mol x L(-1) hydrochloric acid and 1 mol x L(-1) potassium bromide solution and 1 mol x L(-1) potassium nitrate solution (1:1). Bromine is electrogenerated at the anode and reacts with the andrographolide. The number of electrons involved in the eleatrode reaction is 2. Purity of andrographolide is 99.76% compared with 99.77% utilizing area normalization method by HPLC. The RSD are 0.33% and 0.02% respectively. The results from two methods are consistent, so the determination of chemical purity of andrographolide by coulometric titration method is scientific and feasible. The method is rapid, simple, convenient, sensitive and accurate. The reference material is not essential in the method. The method is suitable for determination of chemical purity of andrographolide.
Chromatography, High Pressure Liquid ; Diterpenes ; analysis ; isolation & purification ; Electrolysis ; Electrolytes ; chemistry ; Indicators and Reagents ; chemistry ; Linear Models ; Reproducibility of Results

PURPOSE: The purpose of this study was to investigate the corrosion behaviors of dental implant alloy after micro-sized surface modification in electrolytes containing Mn ion. MATERIALS AND METHODS: Mn-TiO₂ coatings were prepared on the Ti-6Al-4V alloy for dental implants using a plasma electrolytic oxidation (PEO) method carried out in electrolytes containing different concentrations of Mn, namely, 0%, 5%, and 20%. Potentiodynamic method was employed to examine the corrosion behaviors, and the alternating-current (AC) impedance behaviors were examined in 0.9% NaCl solution at 36.5℃±1.0℃ using a potentiostat and an electrochemical impedance spectroscope. The potentiodynamic test was performed with a scanning rate of 1.667 mV s⁻¹ from −1,500 to 2,000 mV. A frequency range of 10⁻¹ to 10⁵ Hz was used for the electrochemical impedance spectroscopy (EIS) measurements. The amplitude of the AC signal was 10 mV, and 5 points per decade were used. The morphology and structure of the samples were examined using field-emission scanning electron microscopy and thin-film X-ray diffraction. The elemental analysis was performed using energy-dispersive X-ray spectroscopy. RESULT: The PEO-treated surface exhibited an irregular pore shape, and the pore size and number of the pores increased with an increase in the Mn concentration. For the PEO-treated surface, a higher corrosion current density (I(corr)) and a lower corrosion potential (E(corr)) was obtained as compared to that of the bulk surface. However, the current density in the passive regions (I(pass)) was found to be more stable for the PEO-treated surface than that of the bulk surface. As the Mn concentration increased, the capacitance values of the outer porous layer and the barrier layer decreased, and the polarization resistance of the barrier layers increased. In the case of the Mn/Ca-P coatings, the corroded surface was found to be covered with corrosion products. CONCLUSION: It is confirmed that corrosion resistance and polarization resistance of PEO-treated alloy increased as Mn content increased, and PEO-treated surface showed lower current density in the passive region.
Alloys ; Corrosion ; Dental Implants ; Dielectric Spectroscopy ; Electric Impedance ; Electrolytes ; Manganese ; Methods ; Microscopy, Electron, Scanning ; Plasma ; Spectrum Analysis ; X-Ray Diffraction

PURPOSE: Paraquat (1,1-dimethyl-4,4'-bipyridylium chloride) is widely a used non- selective herbicide. In spite of efforts to improve the outcome in patients poisoned with paraquat, the mortality rates still remains high. The purpose of this study is to identify initial stat laboratory parameters which can affect the survival rate of these patients. METHODS: A retrospective analysis by chart review was done on 67 patients who had ingested paraquat and who had presented to the Emergency Medical Center of Chonnam University Hospital from June 1997 to July 2001. RESULTS: The results were as follows: 1) Survivors were significantly younger than the nonsurvivors (38 years vs 44 years, p=0.03). The volume of paraquat ingested by survivors was significantly smaller than that ingested by the deceased (1 mouthful vs 3 mouthfuls, p<0.001). 2) The WBC count and the levels of serum AST, BUN and serum creatinine in the deceased were significantly higher than those in the survivors. The levels of serum potassium and bicarbonate, arterial pH, and base excess in survivors were significantly higher than those in the deceased. 3) A multivariate analysis revealed that serum creatinine, serum potassium, and arterial base excess were associated with the fatality rate. CONCLUSION: Initial stat laboratory parameters including arterial blood gas analysis, renal function test, and serum electrolytes could be used to predict the outcome of patients poisoned with paraquat. However, the development of readily applicable and reliable indices predicting outcome is desired for the future.
Blood Gas Analysis ; Creatinine ; Electrolytes ; Emergencies ; Humans ; Hydrogen-Ion Concentration ; Jeollanam-do ; Mortality ; Mouth ; Multivariate Analysis ; Paraquat* ; Potassium ; Retrospective Studies ; Survival Rate* ; Survivors

BACKGROUND: Bupivacaine blocks the sodium channels and inhibits the depolarization of myocardium. The inward sodium current (INa) could be activated by decreasing potassium in the extracellualr spaces. We hypothesized that the bupivacaine dose which creates the cardiac toxicity may have the correlation with serum potassium concentration which is in the normal range. METHODS: After the dogs were anesthetized with pentobarbital, 0.5% bupivacaine was administrated at a rate of 0.5 mg/kg/min until SvO2 decreased to 60% or less, which was defined as the point of cardiac depression in this study. Mean arterial pressure (MAP), heart rate (HR), cardiac output (CO), SvO2, ETCO2, arterial blood gas analysis, serum electrolytes (Na+ , K+ , Ca2+ ), and ECG were taken at baseline and cardiac depression point. Infused bupivacaine dose and blood concentration to make cardiac depression were taken. RESULTS: PaO2, PaCO2, pH, serum electrolytes (Na+ , K+ , Ca2+ ) were within nornal range at baseline and cardiac depression point. MAP, HR, CO, SvO2, and ETCO2 were within normal range at baseline point but decreased significantly at cardiac depression point. Bupivacaine made QRS duration on EKG to prolong significantly. Infused bupivacaine dose to make cardiac depression had an strong negative correlation with concentration of serum potassium (P < 0.05, rs: -. 501). CONCLUSIONS: We conclude that the increase of serum potassium concentration enhances the cardiotoxic effects of bupivacaine, even though it is in the normal range.
Anesthetics ; Animals ; Arterial Pressure ; Blood Gas Analysis ; Bupivacaine ; Cardiac Output ; Depression ; Dogs* ; Electrocardiography ; Electrolytes ; Heart Rate ; Hydrogen-Ion Concentration ; Myocardium ; Pentobarbital ; Potassium* ; Reference Values ; Sodium ; Sodium Channels