Background: Burn injuries are a major global health issue, causing significant morbidity and mortality. Negative-pressure wound therapy (NPWT) is a common treatment that aids fluid drainage and enhances blood flow, thereby promoting wound healing. However, its effects on wound healing parameters like fibroblast growth factor-2 (FGF2) and platelet-derived growth factor (PDGF) in deep dermal burns are not fully elucidated. This study aimed to compare NPWT with conventional treatments, namely 0.9% sodium chloride (NaCl) and silver sulfadiazine (SSD), in terms of their effects on FGF2 and PDGF levels in burn wounds. Methods: Seventy-two fresh tissue samples from Yorkshire pig deep dermal burn models were divided into three treatment groups: NPWT, NaCl, and SSD. Wounds were photographed and observed macroscopically. Tissue samples were collected on days 3, 7, 14, and 21 and analyzed using enzyme-linked immunosorbent assay for further statistical analysis. Results: Macroscopic observation indicated better epithelialization and granulation in the NPWT group, with minimal wound contraction. Although differences across the treatment groups were insignificant, NPWT maintained higher and more stable FGF2 levels, peaking on days 7 and 21 (780.62±353.88 ng/mL and 504.31±254.25 ng/mL), with significant delta (Δ) value increases in the later stages (P=0.042), which were not observed in the NaCl and SSD groups. PDGF concentrations showed insignificant differences across all treatment groups, with notable delta changes in the SSD group (P=0.018). Conclusion NPWT demonstrated superior wound healing performance compared to conventional treatments. In addition to favorable macroscopic findings, NPWT potentially maintained FGF2 levels throughout the wound healing process.

Introduction: Rhabdomyosarcoma (RMS) is the major soft tissue sarcoma in childhood, correlated with high morbidity and mortality. We defined clinico-epidemiological profiles and prognoses on pediatric RMS patients in Indonesia. Methods: This study was conducted retrospectively by examining all medical records of pediatric RMS patients in Dr. Sardjito Hospital, Yogyakarta, Indonesia, for 5 years from 2011 until 2016. Results: There were 21 RMS patients in this study. The median age of our subjects was 6 years and predominantly under 10 years old (71.4%). There was a greater tendency for the disease in males, with a male to female ratio of 2 : 1. The head and neck region was the primary tumor site (52.4%). The most common histological subtype of tumor was embryonal RMS (85.7%). The most common measured diameter for tumor size was ≤ 5 cm (42.9%). Almost half of the patients received combination therapy, including surgery and chemotherapy (47,6%). Overall survival rate was 71.7%. Conclusion: We concluded that the epidemiological profile and prognostic factors of childhood RMS patients in our center are similar to the world reference data and were clinically associated with the mortality rate of our RMS patients, but the findings were not considered statistically significant.

Introduction: Wilms tumor is the major kidney neoplasm in childhood. Approach to therapy gives a 90% 2-year survival rate. However, the mortality rate in developing countries is relatively still high. Research about the clinicopathological profile of Wilms tumor in Indonesia is very limited. This study aimed to investigate the Wilms tumor patients in an Indonesian setting. Methods: This research was a descriptive observational study using a cross-sectional design. Clinical and pathological data were collected from patients’ medical records in Dr. Sardjito General Hospital, from 2011 to 2016. Results: Twenty-five patients were recruited with 52% female subjects. The mean age at the first diagnosis was 38 months. All patients had unilateral tumors. The tumor size was mostly (67%) equal or more than 10 cm. The most frequent symptom was abdominal mass. Ninety-two percent of patients have favorable histology. Most cases (68%) had triphasic morphology. The most common metastasis site of tumor was the liver, followed by the lung, skeletal bone, and spleen. Eighty-four percent of patients received chemotherapy, 80% received surgery, and 28% received radiotherapy. The gender distribution and the most common metastasis site in this study were different compared to previous studies. Conclusion: The clinicopathological profile of Wilms tumor in Yogyakarta, Indonesia, generally matches with other studies from other countries, except the gender distribution and the most common metastasis site. Further prospective studies regarding the prognosis of the patients are urgently needed.