A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.
Animals ; Edwardsiella tarda/genetics/*isolation & purification ; Enterobacteriaceae Infections/diagnosis/microbiology/*veterinary ; Fish Diseases/*diagnosis/microbiology ; Fisheries/*methods ; *Flatfishes ; Multiplex Polymerase Chain Reaction/economics/*veterinary ; Sensitivity and Specificity ; Streptococcal Infections/diagnosis/microbiology/*veterinary ; Streptococcus/genetics/*isolation & purification