1.The Influence of Anticoagulants and Time of Measurements on D-Dimer Determinations.
The Korean Journal of Laboratory Medicine 2004;24(1):7-10
BACKGROUND: We evaluated the effects of anticoagulants used in blood sampling and time of mesurements after blood collection on D-dimer determinations. METHODS: A total of 32 samples of sodium citrated plasma, and 23 samples each of serum and ethylene diamine tetraacetic acid (EDTA) plasma, were studied. D-dimer was measured by enzyme linked fluorescent assay using a VIDAS analyzer. RESULTS: The mean+/-SD (median) of D-dimer titers in citrated plasma, serum, and EDTA plasma samples immediately after blood collection were 282.7+/-201.1 (224.6) microgram/L, 255.1+/-206.8 (197.3) microgram/L, and 313.3+/-230.8 (246.4) microgram/L respectively. The results obtained for the three different sample groups were well correlated. While the upper limit of the reference range for D-dimer for citrated plasma has been set at 500 microgram/L, the cut-off values for serum and EDTA plasma were 400 microgram/L (100%sensitivity and specificity), and 585 microgram/L (100% sensitivity and 94.7% specificity), respectively CONCLUSIONS: In addition to citrated plasma, both serum and EDTA plasma can be used for D-dimer assay. A delay in the time of measurements after blood collection does not influence the results significantly.
Anticoagulants*
;
Edetic Acid
;
Plasma
;
Reference Values
;
Sodium
2.IgG elution method using glycine acid EDTA: comparison to chloroquine method.
Seon Ho LEE ; Young Chul OH ; Ki Hong KIM ; Kyou Sup HAN ; Bok Yun HAN ; Sang In KIM
Korean Journal of Blood Transfusion 1993;4(1):61-66
No abstract available.
Chloroquine*
;
Edetic Acid*
;
Glycine*
;
Immunoglobulin G*
3.Pitfalls in clinical genetics.
Hui-Lin CHIN ; Denise Li Meng GOH
Singapore medical journal 2023;64(1):53-58
With the increasing availability of genetic tests, more doctors are offering and ordering such tests for their patients. Ordering a genetic test appears to be a simple process of filling in paperwork, drawing 3 mL of blood in an ethylenediaminetetraacetic acid tube and receiving a test report. This is identical to sending off a full blood count. However, it is far more complex than that. There are many potential pitfalls, as shown by the increasing number of complaints and lawsuits filed against doctors and allied health staff. Furthermore, clinical genetics involves more than just ordering tests; in fact, focusing on genetic tests alone is a potential pitfall. In this review, we discuss the common pitfalls in clinical genetics and how doctors can avoid these pitfalls to ensure patient safety and to safeguard their practice.
Humans
;
Edetic Acid
;
Fenbendazole
;
Patient Safety
;
Physicians
4.Effect of acidic solutions on the microhardness of dentin and set OrthoMTA and their cytotoxicity on murine macrophage.
Soram OH ; Hiran PERINPANAYAGAM ; Yoon LEE ; Jae Won KUM ; Yeon Jee YOO ; Sang Min LIM ; Seok Woo CHANG ; Won Jun SHON ; Woocheol LEE ; Seung Ho BAEK ; Kee Yeon KUM
Restorative Dentistry & Endodontics 2016;41(1):12-21
OBJECTIVES: To evaluate the effects of three acids on the microhardness of set mineral trioxide aggregate (MTA) and root dentin, and cytotoxicity on murine macrophage. MATERIALS AND METHODS: OrthoMTA (BioMTA) was mixed and packed into the human root dentin blocks of 1.5 mm diameter and 5 mm height. Four groups, each of ten roots, were exposed to 10% citric acid (CA), 5% glycolic acid (GA), 17% ethylenediaminetetraacetic acid (EDTA), and saline for five minutes after setting of the OrthoMTA. Vickers surface microhardness of set MTA and dentin was measured before and after exposure to solutions, and compared between groups using one-way ANOVA with Tukey test. The microhardness value of each group was analyzed using student t test. Acid-treated OrthoMTA and dentin was examined by scanning electron microscope (SEM). Cell viability of tested solutions was assessed using WST-8 assay and murine macrophage. RESULTS: Three test solutions reduced microhardness of dentin. 17% EDTA demonstrated severe dentinal erosion, significantly reduced the dentinal microhardness compared to 10% CA (p = 0.034) or 5% GA (p = 0.006). 10% CA or 5% GA significantly reduced the surface microhardness of set MTA compared to 17% EDTA and saline (p < 0.001). Acid-treated OrthoMTA demonstrated microporous structure with destruction of globular crystal. EDTA exhibited significantly more cellular toxicity than the other acidic solutions at diluted concentrations (0.2, 0.5, 1.0%). CONCLUSIONS: Tested acidic solutions reduced microhardness of root dentin. Five minute's application of 10% CA and 5% GA significantly reduced the microhardness of set OrthoMTA with lower cellular cytotoxicity compared to 17% EDTA.
Cell Survival
;
Citric Acid
;
Dentin*
;
Edetic Acid
;
Humans
;
Macrophages*
;
Pemetrexed
5.Effect of acidic solutions on the microhardness of dentin and set OrthoMTA and their cytotoxicity on murine macrophage.
Soram OH ; Hiran PERINPANAYAGAM ; Yoon LEE ; Jae Won KUM ; Yeon Jee YOO ; Sang Min LIM ; Seok Woo CHANG ; Won Jun SHON ; Woocheol LEE ; Seung Ho BAEK ; Kee Yeon KUM
Restorative Dentistry & Endodontics 2016;41(1):12-21
OBJECTIVES: To evaluate the effects of three acids on the microhardness of set mineral trioxide aggregate (MTA) and root dentin, and cytotoxicity on murine macrophage. MATERIALS AND METHODS: OrthoMTA (BioMTA) was mixed and packed into the human root dentin blocks of 1.5 mm diameter and 5 mm height. Four groups, each of ten roots, were exposed to 10% citric acid (CA), 5% glycolic acid (GA), 17% ethylenediaminetetraacetic acid (EDTA), and saline for five minutes after setting of the OrthoMTA. Vickers surface microhardness of set MTA and dentin was measured before and after exposure to solutions, and compared between groups using one-way ANOVA with Tukey test. The microhardness value of each group was analyzed using student t test. Acid-treated OrthoMTA and dentin was examined by scanning electron microscope (SEM). Cell viability of tested solutions was assessed using WST-8 assay and murine macrophage. RESULTS: Three test solutions reduced microhardness of dentin. 17% EDTA demonstrated severe dentinal erosion, significantly reduced the dentinal microhardness compared to 10% CA (p = 0.034) or 5% GA (p = 0.006). 10% CA or 5% GA significantly reduced the surface microhardness of set MTA compared to 17% EDTA and saline (p < 0.001). Acid-treated OrthoMTA demonstrated microporous structure with destruction of globular crystal. EDTA exhibited significantly more cellular toxicity than the other acidic solutions at diluted concentrations (0.2, 0.5, 1.0%). CONCLUSIONS: Tested acidic solutions reduced microhardness of root dentin. Five minute's application of 10% CA and 5% GA significantly reduced the microhardness of set OrthoMTA with lower cellular cytotoxicity compared to 17% EDTA.
Cell Survival
;
Citric Acid
;
Dentin*
;
Edetic Acid
;
Humans
;
Macrophages*
;
Pemetrexed
6.Effects of citrate pyridoxal 5'-phosphate-TRIS(CPT) antivoagulant on spurious low platelet counts caused by EDTA.
Hee Jung KANG ; Woon Heung SONG ; Han Ik CHO ; Sang In KIM
Korean Journal of Hematology 1991;26(1):47-50
No abstract available.
Blood Platelets*
;
Citric Acid*
;
Edetic Acid*
;
Platelet Count*
;
Pyridoxal*
7.Type and treatment of toxic mushroom poisoning in Korea.
Journal of the Korean Medical Association 2015;58(9):818-824
To eat unidentified or misidentified mushrooms taken from the wild can be very dangerous. In the vast majority of toxic mushroom ingestions in Korea, the mushroom was incorrectly identified. In general, poisoning of toxic mushrooms can be classified into seven types according to the toxins that they contain; amatoxin, gyromitrin, coprine, muscarine, ibotenic acid-muscimol, psilocybin-psilocin and gastrointestinal irritants. When clinicians care for a patient who ingested a toxic mushroom, it is very important to identify what kind of mushroom may have caused a patient's illness. But, in clinical practice, accurate botanical identification of the mushroom can be very difficult. Therefore, for estimating the caused mushroom and adequate treatment of poisoning, clinicians should know the type and treatment of toxic mushroom poisoning.
Agaricales*
;
Edetic Acid
;
Humans
;
Irritants
;
Korea*
;
Muscarine
;
Mushroom Poisoning*
;
Poisoning
8.Chemical Dissolution of Intrahepatic Stones in Vitro.
Young Goo KIM ; Kun Sang KIM ; Jong Beum LEE ; Hyung Jin SHIM ; Sang Shin JOO ; Kyung H LEE ; Byung Gook KWAK ; Su Kyoung CHAE ; Hymn Mee PARK
Journal of the Korean Radiological Society 1995;33(6):945-948
PURPOSE: The in vitro dissolution of intrahepatic stones was evaluated using the various solvent mixtures. MATERIALS AND METHODS: Sixty four intrahepatic stones from 16 patients were used. Four kinds of solvent mixtures(No. 1 = basic buffer + EDTA, No. 2=1 + Sulfobetain-12, No. 3=2 + N-acetylcysteine, No. 4=3 + urea) were used. Dissolution rates were determined by measuring the weight loss of stones after 6, 12, 24, 48 hours incubation periods, respectively. RESULTS: The highest dissolution rates in dissolving intrahepatic stones were achieved with No. 4 solvent mixture(1% W/V EDTA/80mM, Sulfobetain-12/1 M, urea, pH 9.5). CONCLUSION: lntrahepatic stones could be largely dissolved up to about 70% of their initial weight after 48 hours incubation period in vitro.
Acetylcysteine
;
Edetic Acid
;
Humans
;
Hydrogen-Ion Concentration
;
Urea
;
Weight Loss
9.Comparisons of Bayer ADVIA Centaur BNP Assay with Biosite Triage BNP Assay.
Kap Jun YOON ; Ki Jin OH ; Sung Ja IM
Journal of Laboratory Medicine and Quality Assurance 2005;27(1):189-193
BACKGROUND: B-type natriuretic peptide (BNP) has been useful as a diagnostic tool to define heart failure. Recently, the BNP assay was used on an automated immunochemistry platform. Studies included precision, analytical correlation between the Biosite Triage BNP assay and Bayer ADVIA Centaur BNP assay. METHODS: Between February 22, 2005 and March 4, 2005, 66 cases were anal-yzed. For the BNP measurement, 3 mL blood samples were collected in plastic tubes containing EDTA. Precision was analyzed with 20 repeat tests in 3 different control levels. RESULTS: The ADVIA Centaur assay had between-run precision (CV) of 3.9%, 3.7%, and 3.7% at BNP concentrations of 41.39, 420.08, and 1671.73 ng/L, respectively. The correlation between the ADVIA Centaur and Triage was as follows: ADVIA Centaur=0.753(Triage)-21.888 ng/L (r=0.94). At a cutoff of 100 ng/L, however, the diagnostic agreement was 89.4%. CONCLUSIONS: The ADVIA Centaur BNP assay is the first commercially available BNP assay using an automated immunochemistry platform. This assay has good analytical and clinical performances and agreement with the Biosite Triage BNP Assay.
Edetic Acid
;
Heart Failure
;
Immunochemistry
;
Natriuretic Peptide, Brain
;
Plastics
;
Triage*
10.Evaluation of Cobas b 101 HbA1c Analyzer Performance for Point-of-Care Testing.
Hui Jin YU ; Sangeun LIM ; Min Jung KWON ; Hee Yeon WOO ; Hyosoon PARK
Laboratory Medicine Online 2017;7(4):182-188
BACKGROUND: The use of point-of-care (POC) devices for evaluating HbA1c is increasing; accordingly, comparisons between these devices and central laboratory methods are important. In the present study, we evaluated the analytical performance of the cobas b 101 analyzer for POC HbA1c testing. METHODS: The analytical quality of the cobas b 101 system was assessed based on repeatability, within-laboratory precision, linearity, and lot-to-lot reproducibility. Two specimen types, i.e., EDTA whole blood and capillary blood, were examined using the cobas b 101 system and the Variant II Turbo instrument. RESULTS: The coefficient of variation for within laboratory precision was 5.22% for a normal HbA1c level and 2.56% for a higher HbA1c level. The method showed good linearity, with a coefficient of correlation of 0.990. In a comparison of two different HbA1c disk lots, a strong correlation (r=0.986) and a mean %difference of −2.9% were observed. The cobas b 101 results using EDTA whole blood were strongly correlated with the Variant II Turbo results (r=0.958), with a mean %difference of 0.8%; the cobas b 101 results using capillary blood were strongly correlated with the Variant II Turbo results, using EDTA whole blood (r=0.976), with a mean %difference of 2.0%. A comparison between HbA1c levels in EDTA whole blood and capillary blood obtained using the cobas b 101 showed a strong correlation (r=0.985) and a mean %difference of 1.3%. CONCLUSIONS: The cobas b 101 analyzer is convenient for the measurement of HbA1c levels for diabetes management.
Capillaries
;
Edetic Acid
;
Methods
;
Point-of-Care Systems*
;
Point-of-Care Testing*