No abstract available.
Ecthyma ; Humans ; Infant

No abstract available.
Adolescent* ; Ecthyma* ; Humans ; Pseudomonas aeruginosa

No abstract available.
Ecthyma ; Humans ; Lymphohistiocytosis, Hemophagocytic ; Pseudomonas aeruginosa

No abstract available.
Diagnosis* ; Ecthyma* ; Humans ; Kidney Failure, Chronic*

We have previously observed that a sequence in coat protein (CP) ORF of Turnip yellow mosaic virus (TYMV) is required for efficient replication of the virus. The sequence was predicted to take a stem-loop structure, thus termed SL2. While examining various SL2 mutants, we observed that all the modifications resulting in extension of translation beyond the CP ORF significantly suppressed subgenomic RNA accumulation. The genomic RNA level, in contrast, was not affected. Introduction of an in-frame stop codon in the CP ORF of these constructs restored the level of subgenomic RNA. Overall, the results suggest that the read-through makes the subgenomic RNA unstable.
Animals ; Brassica napus ; Codon, Terminator ; Ecthyma, Contagious ; RNA ; Tymovirus ; Viruses

Bacteriological study, including antibiotic sensitivity tests, of 145 patients with pyodermas such as impetigo, superficial folliculltis, deep folliculitis, ecthyma, paronychia, cellulitis and secondary skin infections, was carried out during the period from June, 1981 to September, 1982 at the Department of Dermatology, Han II hospital. (countinued..)
Cellulitis ; Dermatology ; Ecthyma ; Folliculitis ; Humans ; Impetigo ; Paronychia ; Pyoderma* ; Skin

Systemic candidiasis is a fatal fungal infection and its diagnosis is often difficult because Candida organism may be cultured from blood specimens in only 25% of the patients. The triad of systemic candidiasis is fever, papular rash, and diffuse muscle tenderness. Therefore, the recognition of the characteristic cutaneous lesions of disseminated candidiasis allows earlier diagnosis and treatment. The cutaneous lesion usually consists of red or purpuric papules, some with pale centers, abscess, necrotic lesion, hemorrhage and skin lesion mimicking ecthyma gangrenosum. We report a rare case of disseminated candidiasis presented as multiple subcutaneous nodule-typed skin lesions.
Abscess ; Candida ; Candidiasis* ; Diagnosis ; Ecthyma ; Exanthema ; Fever ; Hemorrhage ; Humans ; Myalgia ; Skin

Turnip yellow mosaic virus (TYMV) is a non-enveloped icosahedral virus that has a single 6.3 kb positive-strand RNA as a genome. Previously, it was observed that the recombinant construct TY-eGFP2, where an eGFP gene was inserted at the position downstream of the coat protein (CP) ORF of TYMV genome, barely replicated. The inhibition of replication was relieved by insertion of an additional copy of the 3' quarter of the CP ORF after the foreign sequence. In this study, we have examined if the 3' quarter of the CP ORF contains any replication elements. M-fold analysis predicted three stem-loop structures in this region. Analysis of the TY-eGFP2 constructs containing one or two of these stem-loop structures indicates that the secondary structure predicted in the region between nt-6139 and nt-6181, termed SL2, is essential for TYMV replication. The critical role of SL2 was confirmed by the observation that deletion of the 3' quarter of the CP ORF from the wild-type TYMV genome nearly abolished replication and that insertion of SL2 into the deletion mutant restored the replication. Mutations disrupting the stem of SL2 greatly reduced viral RNA replication, indicating that the secondary structure is essential for the enhancing activity.
Animals ; Brassica napus ; Coat Protein Complex I ; Ecthyma, Contagious ; Genome ; RNA ; RNA, Viral ; Tymovirus ; Viruses

The primary clue for locating protein-coding regions is the open reading frame and the determination of ORFs (Open Reading Frames) is the first step toward the gene prediction, especially for prokaryotes. In this respect, we have developed a web-based ORF search tool called ORF Miner. The ORF Miner is a graphical analysis utility which determines all possible open reading frames of a selectable minimum size in an input sequence. This tool identifies all open reading frames using alternative genetic codes as well as the standard one and reports a list of ORFs with corresponding deduced amino acid sequences. The ORF Miner can be employed for sequence annotation and give a crucial clue to determination of actual protein-coding regions.
Amino Acid Sequence ; Animals ; Ecthyma, Contagious ; Genetic Code ; Open Reading Frames ; Resin Cements

Turnip yellow mosaic virus (TYMV) is a non-enveloped icosahedral virus composed of 20 kDa single coat proteins. In this study, we modified the TYMV coat protein (CP) ORF by inserting an oligonucleotide linker corresponding to T7, HSV, Tat, (Arg)9, or (RxR)4 peptide at the 5'-end of the CP ORF and examined its effect on replication, RNA packaging, and virion assembly. The results showed that the constructs containing (Arg)9 and (RxR)4 sequences were barely capable of replication. The TYMV constructs containing T7 and Tat peptide produced virions that co-migrated with wild-type virions. However, the insertion of T7 and Tat sequences impaired genomic RNA (gRNA) accumulation and packaging, respectively. When only the CP gene was expressed, CPs with (Arg)9 or (RxR)4 successfully produced virus-like particles whose mobility was comparable to that of wild type. In the case of CP having a HSV tag, the virion band was not detected, although a sufficient amount of CP was produced. This indicates that CP with the HSV tag failed to assemble into virions. Overall, the results suggest that TYMV replication, RNA packaging and virion assembly are strongly influenced by the insertion sequence.
Animals ; Brassica napus* ; Capsid Proteins ; Ecthyma, Contagious ; Product Packaging* ; RNA* ; Tymovirus* ; Virion*