1.Hydatid cyst/cystic echinococcosis: anatomical and surgical nomenclature and method to quantify the cyst content solidification.
Chinese Medical Journal 2011;124(18):2806-2812
The terminology related to the hydatid cyst is sometimes inappropriate and wrong designations are used, based on incorrect concepts. "Hydatid cyst" is the larval form of the tapeworms of the genus Echinococcus granulosus, a parasite found in the small intestine of carnivores. The disease, called cystic echinococcosis, is among the most neglected parasitic diseases despite the development of new drugs and other treatment modalities. Although all of us know the vital cycle of the parasite and the different aspects of the disease, the designations around the parasite, its evolution and some therapeutic procedures are not uniform. It would be useful, for all of us, to use the same nomenclature and it is absolutely necessary that the nomenclature is correct and universally accepted. In this paper I remember the correct terms related to all aspects of the hydatid cyst's nomenclature, including especially the anatomical and surgical terminology, as well as the criteria to define the cyst inactivity and the way to know when the cyst range the inactive stage after therapy.
Animals
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Echinococcosis
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epidemiology
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metabolism
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pathology
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surgery
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Echinococcus granulosus
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anatomy & histology
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classification
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Humans
2.Analysis of protoscoleces-specific antigens from Echinococcus granulosus with proteomics combined with Western blot.
Biomedical and Environmental Sciences 2012;25(6):718-723
OBJECTIVETo establish and optimize the proteomic analysis of protoscoleces-specific antigens from Echinococcus granulosus. To provide a foundation for identifying specific antigens in the soluble proteins of E. granulosus protoscoleces for further research.
METHODSBrood capsules were collected aseptically from fertile E. granulosus cysts from the livers of an infected patient. The fertile E. granulosus cysts were fractured, and protoscoleces were collected by centrifugation. The soluble proteins of protoscoleces were acquired using the 2D Quant kit according to the manufacturer's instructions. We employed two-dimensional electrophoresis (2-DE) combined with immunoblot assay (Western blot) to analyze the soluble components of E. granulosus protoscoleces antigens. The 2-DE and immunoblot maps obtained were analyzed with PDQuest 8.0 image analysis software.
RESULTSAbout 233 soluble protein spots were identified with Coomassie-stained gels. Most of the proteins had a molecular weight of 16,000 Da to 117,000 Da, and an isoelectric point value of 3.0 to 10.0. 2-DE immunoblot was conducted and 57 specific antigen spots were observed, among which 23 spots were identified.
CONCLUSION2-DE combined with Western blot is the key to successful proteomic analysis and presents a new possibility for searching the specific E. granulosus protoscoleces antigens.
Animals ; Antigens, Helminth ; chemistry ; metabolism ; Blotting, Western ; methods ; Echinococcus granulosus ; classification ; metabolism ; Electrophoresis, Gel, Two-Dimensional ; Gene Expression Regulation ; Helminth Proteins ; isolation & purification ; Proteomics ; methods
3.Molecular characterization of a signal-regulated kinase homolog from Echinococcus granulosus.
Jing LI ; Chuan-Shan ZHANG ; Guo-Dong LÜ ; Jun-Hua WANG ; Hao WEN ; Gen-Qiang YAN ; Xu-Fa WEI ; Ren-Yong LIN
Chinese Medical Journal 2011;124(18):2838-2844
BACKGROUNDCystic echinococcosis due to Echinococcus granulosus (E. granulosus) is one of the most important chronic helminthic diseases, especially in sheep/cattle-raising regions. The larval stage of the parasite forms a cyst that grows in the liver, lung, or other organs of the host. To ensure a long life in the host tissues, the parasite establishes complex inter-cellular communication systems between its host to allow its differentiation toward each larval stage. Recent studies have reported that this communication is associated with the extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase cascade in helminth parasites, and in particular that these protein kinases might serve as effective targets for a novel chemotherapy for cystic echinococcosis. The aim of the present study investigated the biological function of a novel ERK ortholog from E. granulosus, EgERK.
METHODSDNA encoding EgERK was isolated from protoscolices of E. granulosus and analyzed using the LA Taq polymerase chain reaction (PCR) approach and bioinformatics. Reverse transcription PCR (RT-PCR) was used to determine the transcription level of the gene at two different larval tissues. Western blotting was used to detect levels of EgERK protein. The expression profile of EgERK in protoscolices was examined by immunofluorescence.
RESULTSWe cloned the entire Egerk genomic locus from E. granulosus. In addition, two alternatively spliced transcripts of Egerk, Egerk-A, and Egerk-B were identified. Egerk-A was found to constitutively expressed at the transcriptional and protein levels in two different larval tissues (cyst membranes and protoscolices). Egerk-A was expressed in the tegumental structures, hooklets, and suckers and in the tissue surrounding the rostellum of E. granulosus protoscolices.
CONCLUSIONSWe have cloned the genomic DNA of a novel ERK ortholog from E. granulosus, EgERK (GenBank ID HQ585923), and found that it is constitutively expressed in cyst membrane and protoscolex. These findings will be useful in further study of the biological functions of the gene in the growth and development of Echinococcus and will contribute to research on novel anti-echinococcosis drug targets.
Animals ; Blotting, Western ; Computational Biology ; DNA, Helminth ; genetics ; Echinococcus granulosus ; enzymology ; genetics ; Genome, Helminth ; genetics ; Helminth Proteins ; genetics ; metabolism ; Polymerase Chain Reaction
4.Echinococcus granulosus cyst fluid(EgCF) inhibits the migration and phagocytic function of mouse macrophages induced by LPS via inducing cytoskeletal rearrangement.
Feiming HE ; Dan DONG ; Yuting CHEN ; Yuan LIAO ; Ke LIN ; Jin MENG ; Xiangwei WU ; Xueling CHEN
Chinese Journal of Cellular and Molecular Immunology 2023;39(5):385-390
Objective To investigate the effect of Echinococcus granulosus cyst fluid(EgCF) on the cytoskeletal rearrangement and phagocytosis and the migration of macrophages induced by lipopolysaccharide(LPS). Methods Peritoneal macrophages of C57BL/6 mice were isolated and cultured in vitro, and divided into control group and LPS group and LPS combined with EgCF group. After 48 hours of treatment, filamentous actin (F-actin) changes were observed with rhodamine-labelled phalloidin staining and fluorescence microscopy; TranswellTM chamber was used to test cell migration ability and flow cytometry to test cell phagocytosis. After 1 hour of treatment, PI3K and AKT, phosphorylated AKT (p-AKT), Rac1, guanosine triphospho-Rac1 (GTP-Rac1), WASP and Arp2 protein expressions were detected with Western blot analysis. Results Compared with the control group, after LPS stimulation, macrophages were deformed significantly; pseudopodia increased; actin cytoskeleton increased and was more distributed in pseudopodia; the ability of migration and phagocytosis were significantly improved, and the expression of PI3K, p-AKT, GTP-Rac1, WASP and Arp2 proteins significantly increased. EgCF treatment caused cell shrinkage and disappearance of pseudopodia protrusions of LPS-activated cells, and led to the reduced phagocytic and migratory of cells; the protein expression of PI3K, p-AKT, GTP-Rac1, WASP and Arp2 decreased significantly compared with the LPS group. Conclusion LPS induces the migration and enhances phagocytosis of macrophages while EgCF inhibits these effects, which is related to actin cytoskeleton rearrangement.
Mice
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Animals
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Lipopolysaccharides/pharmacology*
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Echinococcus granulosus/metabolism*
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Proto-Oncogene Proteins c-akt
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Cyst Fluid/metabolism*
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Mice, Inbred C57BL
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Macrophages/metabolism*
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Phagocytosis
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Actins/metabolism*
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Phosphatidylinositol 3-Kinases/metabolism*
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Guanosine Triphosphate/pharmacology*
5.Echinococcus granulosus 14-3-3 protein: a potential vaccine candidate against challenge with Echinococcus granulosus in mice.
Zong Ji LI ; Ya Na WANG ; Qi WANG ; Wei ZHAO
Biomedical and Environmental Sciences 2012;25(3):352-358
OBJECTIVETo investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3.
METHODSICR mice were subcutaneously immunized three times with rEg14-3-3, followed by the challenge with Echinococcus granulosus protoscoleces intraperitoneally and then sacrificed after six months of post-challenge to detect the proliferation of splenocytes by MTT assay, and to measure the secretion of IL-2, IL-4, IL-10, and IFN-γ by ELISA. The rate of reduced hydatid cyst and the levels of IgE, IgG and IgG subclasses in sera were examined.
RESULTSMice vaccinated with rEg14-3-3 and challenged with protoscoleces revealed significant protective immunity of 84.47%. ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a. Splenocytes from mice immunized with rEg14-3-3 showed a significant proliferation response. The secretion of IFN-γ and IL-2 increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 and IL-10 levels between vaccinated and control mice.
CONCLUSIONThe results indicate that the rEg14-3-3 vaccine could induce a high level of protective immunity as a promising vaccine candidate to prevent cystic echinococcosis.
14-3-3 Proteins ; genetics ; metabolism ; Animals ; Blotting, Western ; Cell Proliferation ; Cytokines ; genetics ; metabolism ; Echinococcosis ; prevention & control ; Echinococcus granulosus ; genetics ; metabolism ; Enzyme-Linked Immunosorbent Assay ; Gene Expression Regulation ; physiology ; Mice ; Spleen ; cytology ; Vaccines ; immunology
6.Genetic variation of the 8-kDa glycoprotein family from Echinococcus granulosus, Taenia multiceps and Taenia hydatigena.
Wan-Zhong JIA ; Hong-Bin YAN ; Zhong-Zi LOU ; Xing-Wei NI ; Hong-Xia LIU ; Hong-Min LI ; Ai-Jiang GUO ; Bao-Quan FU
Chinese Medical Journal 2011;124(18):2849-2856
BACKGROUNDEchinococcosis, coenurosis and cysticercosis are debilitating diseases which prevail in China. Immunological diagnosis of metacestodosis is important in disease control. The 8-kDa glycoproteins from taeniid cestodes have successfully been used for diagnosis of human cysticercosis in immunological assays. The aim of the present study was to investigate genetic variations and phylogenetic relationships of the 8-kDa proteins for evaluating the possibility of utilizing these proteins as diagnostic antigens for other metacestode infections.
METHODSThe genes and complementary DNAs (cDNAs) encoding the 8-kDa proteins from Echinococcus (E.) granulosus, Taenia (T.) multiceps and T. hydatigena were amplified using PCR method. Their amplicons were cloned into the vector pMD18 and the positive clones were sequenced. Sequence data were analyzed with the SeqMan program, and sequence homology searches were performed using the BLAST program. Alignments were conducted using the ClustalX program, and the phylogenetic analyses were performed with the Protein Sequences Program and the Puzzle Program using the Neighbor-joining method.
RESULTSFifteen, 18 and 22 different genomic DNA sequences were identified as members of the 8-kDa protein gene family from E. granulosus, T. multiceps and T. hydatigena, respectively. Eight, four and six different cDNA clones respectively from E. granulosus, T. multiceps and T. hydatigena were characterized. Analysis of these sequences revealed 54 unique 8-kDa protein sequences. Phylogenetic trees demonstrated that the taeniid 8-kDa proteins are clustered into eight clades at least: Ts18, Ts14, TsRS1, TsRS2, T8kDa-1, T8kDa-2, T8kDa-3 and T8kDa-4.
CONCLUSIONWe found that the gene family encoding for the taeniid 8-kDa antigens is comprised of many members with high diversity, which will provide molecular evidence for cross-reaction or specific reaction among metacestode infections and may contribute to the development of promising immunological methods for diagnosis of metacestodosis.
Amino Acid Sequence ; Animals ; DNA, Helminth ; genetics ; Echinococcus granulosus ; genetics ; metabolism ; Genetic Variation ; genetics ; Glycoproteins ; chemistry ; classification ; genetics ; Helminth Proteins ; chemistry ; classification ; genetics ; Molecular Sequence Data ; Phylogeny ; Sequence Homology, Amino Acid ; Taenia ; genetics ; metabolism
7.Serological and Molecular Characteristics of the First Korean Case of Echinococcus multilocularis.
Jin Sook JEONG ; Sang Young HAN ; Young Hoon KIM ; Yasuhito SAKO ; Tetsuya YANAGIDA ; Akira ITO ; Jong Yil CHAI
The Korean Journal of Parasitology 2013;51(5):595-597
In December 2011, we reported an autochthonous case of Echinococcus multilocularis infection in a 42-year-old woman in Korea. The diagnosis was based on histopathological findings of the surgically resected liver cyst. In the present study, we evaluated the serological and molecular characteristics of this Korean E. multilocularis case. The patient's serum strongly reacted with affinity-purified native Em18 and recombinant Em18 antigens (specific for E. multilocularis) but negative for recombinant antigen B8/1 (reactive for Echinococcus granulosus). In immunoaffinity chromatography, the serum also strongly reacted with E. multilocularis and only weakly positive for E. granulosus. We determined the whole nucleotide sequence of cox1 (1,608 bp) using the paraffin-embedded cystic tissue which was compared with E. multilocularis isolates from China, Japan, Kazakhstan, Austria, France, and Slovakia. The Korean case showed 99.8-99.9% similarity with isolates from Asia (the highest similarity with an isolate from Sichuan, China), whereas the similarity with European isolates ranged from 99.5 to 99.6%.
Adult
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Animals
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Antibodies, Helminth/*blood
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Antigens, Helminth/genetics/*immunology/metabolism
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Base Sequence
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Echinococcosis, Hepatic/*immunology/parasitology
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Echinococcosis, Pulmonary/diagnosis/genetics/immunology
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Echinococcus granulosus/genetics/immunology
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Echinococcus multilocularis/genetics/*immunology/isolation & purification
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Electron Transport Complex IV/genetics
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Female
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Humans
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Mitochondria/genetics
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Molecular Sequence Data
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Republic of Korea
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Sequence Analysis, DNA
8.Pericyst may be a new pharmacological and therapeutic target for hydatid disease.
Xiang-Wei WU ; Xue-Ling CHEN ; Shi-Jie ZHANG ; Xi ZHANG ; Hong SUN ; Xin-Yu PENG
Chinese Medical Journal 2011;124(18):2857-2862
BACKGROUNDMost hydatid cysts with calcified walls are biologically and clinically silent and inactive. Transforming growth factor-beta 1 (TGF-β1) plays a critical role in the calcification process of cells. The aim of this study was to assess the effect of modulating TGF-β1 signaling on the calcification of hydatid cysts.
METHODSPericyst cells isolated from hepatic hydatid cysts were cultured with osteogenic media. These cells were assessed for alkaline phosphatase activity and mineralization capacity using Alizarin Red staining. Cells were also treated with recombinant human TGF-β1 and TGF-β inhibitor, and the expression profiles of osteoblast markers (RUNX2, osterix, and osteocalcin) were analyzed using Western blotting. The effects of inhibiting TGF-β1 signaling on calcification of pericyst walls were assessed using different doses of TGF-β inhibitor for 7 weeks in a preclinical disease model of liver cystic echinococcosis.
RESULTSCells within the pericyst displayed high levels of alkaline phosphatase activity and mineralized nodule formation, as induced by osteogenic media. These activities, as well as expression profiles of osteoblast markers (RUNX2, osterix, and osteocalcin) could be inhibited by addition of recombinant human TGF-β1 (rhTGF-β1) and enhanced by TGF-β inhibitor. In the animal model of cystic echinococcosis, inhibition of TGF-β1 signaling increased calcification of the pericyst wall, which was associated with decreased cyst load index and lower viability of protoscoleces.
CONCLUSIONSCells within the pericysts adopt an osteoblast-like phenotype and have osteogenic potential. Inhibition of TGF-β1 signaling increases hydatid cyst calcification. Pharmacological modulation of calcification in pericysts may be a new therapeutic target in the treatment of hydatid disease.
Animals ; Blotting, Western ; Calcification, Physiologic ; drug effects ; Cell Differentiation ; drug effects ; Core Binding Factor alpha Subunits ; metabolism ; Echinococcosis ; metabolism ; pathology ; Echinococcus granulosus ; pathogenicity ; Enzyme Inhibitors ; pharmacology ; Humans ; Male ; Mice ; Osteoblasts ; cytology ; Osteocalcin ; metabolism ; Recombinant Proteins ; pharmacology ; Sp7 Transcription Factor ; Transcription Factors ; metabolism ; Transforming Growth Factor beta1 ; antagonists & inhibitors ; metabolism