Objective: To investigate the acute toxicity of cadmium and lead on larvae of two tropical Chironomid species, Chironomus kiiensis (C. kiiensis) Tokunaga and Chironomus javanus (C. javanus) Kieffer. Methods: Different larval instars (first-fourth) were exposed using a static non-replacement testing procedures to various concentrations of cadmium and lead. Results:In general, younger larvae (first and second instars) of both species were more sensitive to both metals than older larvae (third and forth instars). The toxic effects of the metals on C. kiiensis and C. javanus were influenced by the age of the larvae (first to fourth instars), types of metals (cadmium or lead) and duration of larval exposure (24, 48, 72 and 96 h) to the metals. Conclusions: Cadmium was more toxic to the chironomids than lead and C. javanus was significantly more sensitive to both metals than C. kiiensis (P<0.05).