*Blood Coagulation Tests ; *Hemophilia A ; *Child

The quantum effect of carrier distribution in nanoscale SOI MOSFETs is evident and must be taken into consideration in device modeling and simulation. In this paper, a backpropagation neural network was applied to predict the quantum density of carriers from the classical density, and the influence of the network structure on training speed and accuracy was studied. It was concluded that a carefully trained neural network with two hidden layers using the Levenberg-Marquardt learning algorithm could predict the carrier quantum density of SOI MOSFETs in very good agreement with Schrödinger Poisson equations.

Objective: To investigate the antimicrobial resistance in and multilocus sequence typing (MLST) of Campylobacter jejuni in meat and meat products in Liaoning Province, so as to provide insights into the management of C. jejuni infection. Methods: Nine C. jejuni isolates from meat and meat products in 2020 were subjected to whole genome sequencing, and the minimum inhibitory concentration was measured with the agar dilution method. MLST of C. jejuni isolates was performed with the microbial resistance mechanism traceability reference database and analysis system. Results: Six drug-resistant C. jejuni isolates were detected, and there were four multidrug-resistant isolates. There were six C. jejuni isolates resistant to tetracycline, five isolates resistant to nalidixic acid, four isolates resistant to ciprofloxacin, two isolates resistant to florfenicol, one isolate resistant to gentamicin and one isolate resistant to streptomycin. Nine C. jejuni isolates showed sensitive to azithromycin, chloramphenicol and clindamycin. MLST identified six ST types in nine C. jejuni isolates, with ST45 and ST2274 as the predominant type, and detected one isolate with unclassified ST type. Phylogenetic analysis showed that KW028 and KW029 of ST45 type were closely related and had high homology, and KW040 and KW042 of ST2274 type were closely related with high homology, while KW007 of ST6701 type was closely related to KW040 and KW042 of ST2274 type, with only one pgm housekeeper gene in difference. Conclusions High resistance to tetracycline, nalidixic acid and ciprofloxacin was detected in nine C. jejuni isolates from meat and meat products, and ST45 and ST2274 were predominant ST types of C. jejuni.

OBJECTIVETo find out the mechanism of Tangfukang on the diabetic nephropathy(DN).

METHODA Model of streptorotocin diabetic rats was used. The expressions of the AGEs, ICAM-1, IV-C and FN were observed in renal cortex of diabetic rats with immunohistochemical method. The level of the IL-1 beta in blood serum was measured by radioimmunoassay method and the level of TNF was determined in blood serum by ELISA method. Aminoguanidine was selected as the control medicine which could inhibit the expression of monenzymatic glycosylation end products of protein. All the results were compared to analyze the effect of Tangfukang on monenzymatic glycosylation of protein and cytokine expression in early diabetic nephropathy rats.

RESULTSBoth Tangfukang and aminoguanidine significantly decreased the expression of AGEs, but Tangfukang was greatly superior to aminoguanidine in suppressing the expression of cytokine like ICAM-1, IL-1 beta, TNF-alpha and the ingredients of extracellular matrix like IV-C, FN.

CONCLUSIONIt may be the partical mechanism of Tangfukang to suppress the monenzymatic glycosylation of protein and to reduce the expression of cytokine in diabetes. The effect of Tangfukang is superior to that of aminoguanidine.

Adrenal Cortex ; metabolism ; Animals ; Astragalus membranaceus ; chemistry ; Capsules ; Diabetes Mellitus, Experimental ; metabolism ; Diabetic Nephropathies ; metabolism ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; pharmacology ; Glycation End Products, Advanced ; metabolism ; Intercellular Adhesion Molecule-1 ; metabolism ; Interleukin-1 ; blood ; Ligustrum ; chemistry ; Male ; Phellodendron ; chemistry ; Plants, Medicinal ; chemistry ; Rats ; Rats, Wistar ; Tumor Necrosis Factor-alpha ; metabolism

Objective To explore the etiologies of persistent hyperbilirubinemia in follow-up patients after liver transplantation. Methods The clinical data of 527 follow-up patients after liver transplantation at the department of hepatobiliary surgery in Peking University People's Hospital between May 2000 and Dec 2009 were analyzed retrospectively. Results Sixty-five patients experienced persistent hyperbilirubinemia,there were 34 cases of biliary complications (52.3％ ),14 cases with drug-induced liver injury(21.5％ ),6 cases with hepatitis B or C recurrence(9.2％ ),4 cases with acute rejection (6.2％ ) 3 cases with inflammatory stenosis of the duodenal papilla ( 4.6％ ),2 cases with hepatic carcinoma recurrence(3.1％ ),1 case with neomalignancy and 1 case with unknown reason.Treatment included use of liver protectant and choleretics,the adjustment and replacement of anti-rejection drugs,cholelithotomy by choledochoscope,PTCD,placement of biliary stent and re-operation.Of all the 65 cases 50 patients were cured or in stable condition,15 patients died. Conclusions Jaundice is a common clinical manifestation after liver transplantation with multiple and complicated causes.Diffuse stricture of intrahepatic biliary tract predicts poor prognosis,drug-related liver injury was less common cause of hyperbilirubinemia and often misdiagnosed.

Objective To research the method of Chronic hepatic injury modeling in mice induced by D -galactosamine and lipopolysaccharide combination . Methods Injected D-galactosamine ( 30 mg/mL ) and lipopolysaccharide ( 2μg/mL ) combination by intraperitoneal injection , two days at a time for 8 weeks .Monitored variation of diet and weight; detected serum level of alanine aminotransferase ( ALT ) and aspartate aminotransferase (AST), been put to death in mice and removed the liver tissue .strained hepatic tissue by the HE and Masoon dye to observe Liver tissue structure and cellular morphology and the degree of fibrosis .Results Lipopolysaccharide and D-galactosamine combination resulted in ALT rise , hepatocyte degeneration and necrosis ,collagen fiber hyperplasia obviously . Conclusion D-galactosamine and Lipopolysaccharide combination could induce mice chronic hepatic injury modeling .

Aim To target the key proteins of TLR4 signaling pathway to sereen the compounds of Blumea baisamifera ( L.) DC.in order to explore the anti-in- flammatorv active components and the mechanism of action of key proteins targeting TLR4 signaling pathway in Blumea balsamifera( L.) DC.Methods The effects of ergosterol peroxide ( EP) on cell activity, LPS in¬duced inflammatory factor secretion and NF-kB related protein expression were detected by MTT, EL1SA, Western blot and Subcellular Structural Localization.Results The results showed EP, (3-sitosterol, 16- kaurene, carosterol, luteolin, ergosterol, hyperin and genkwa had more optimal interconnection score.EP (2.3 x 10"5 ~ 9.3 x 10 5 mol • L"1) inhibited the degradation and phosphorylation of inflammatory pro¬tein IkB and phosphorylation of NF-kB p65 down¬stream of TLR4 signaling pathway induced by LPS in BAW264.7 cells,and further inhibit the entrv of NF-kB p65 into the nucleus, thus inhibiting the secretion of cytokines IL-lp and 1L-6.Conclusions The antiin¬flammatory components of Blumea balsamifera ( L.) DC are identified by molecular docking technology and ac-tivity detection technique, and the anti-inflammatory action anrl mechanism of EP as active substances are e- luci dated.

A Triazophos-degrading strain, Klebsiella sp. E6, was identified by soil enrichment technology from the soil sampled from the vicinity of a factory manufacturing Triazophos (TAP). The nutrient requirement of the strain is simple. It can use TAP as the sole sources of carbon, nitrogen and phosphorus. Comparison of the degradation rates revealed that the strain degraded TAP most effectively when TAP was used as a sole nitrogen source. No inhibition effect occurred when TAP concentration was high as 1000 mg/L in the case of TAP was used as the sole nitrogen source. Analysis of the intermediates of TAP metabolism indicated that TAP is firstly hydrolyzed into 1-phenyl-3-hydroxy-1,2,4-triazole and O,O-diethyl phosphorothioic acid. 1-phenyl-3-hydroxy-1,2,4-triazole was further mineralized into inorganic compounds. A degradation pathway of TAP was proposed. The experiment results demonstrated that the strain has potential in biodegradation of TAP pollutions.
Biodegradation, Environmental ; Environmental Pollutants ; metabolism ; Klebsiella ; isolation & purification ; metabolism ; Organothiophosphates ; metabolism ; Pesticides ; metabolism ; Soil Microbiology ; Triazoles ; metabolism

BACKGROUND: This study was undertaken to validate the use of the modified early warning score (MEWS) as a predictor of patient mortality and intensive care unit (ICU)/ high dependency (HD) admission in an Asian population. METHODS: The MEWS was applied to a retrospective cohort of 1024 critically ill patients presenting to a large Asian tertiary emergency department (ED) between November 2006 and December 2007. Individual MEWS was calculated based on vital signs parameters on arrival at ED. Outcomes of mortality and ICU/HD admission were obtained from hospital records. The ability of the composite MEWS and its individual components to predict mortality within 30 days from ED visit was assessed. Sensitivity, specificity, positive and negative predictive values were derived and compared with values from other cohorts. A MEWS of ≥4 was chosen as the cut-off value for poor prognosis based on previous studies. RESULTS: A total of 311 (30.4%) critically ill patients were presented with a MEWS ≥4. Their mean age was 61.4 years (SD 18.1) with a male to female ratio of 1.10. Of the 311 patients, 53 (17%) died within 30 days, 64 (20.6%) were admitted to ICU and 86 (27.7%) were admitted to HD. The area under the receiver operating characteristic curve was 0.71 with a sensitivity of 53.0% and a specificity of 72.1% in addition to a positive predictive value (PPV) of 17.0% and a negative predictive value (NPV) of 93.4% (MEWS cut-off of ≥4) for predicting mortality. CONCLUSION: The composite MEWS did not perform wel in predicting poor patient outcomes for critical y il patients presenting to an ED.

The purpose of this study was to assess the effect of magnitopuncture stimuli for reducing driver mental stress and fatigue using power spectral analysis of the heart rate variability (HRV) and subjective evaluation. The experiments were divided into A-group and B-group. In both groups the subjects performed the simulator for 90 minutes under a vibration conditions with an erect sitting posture in a silent environment, and magnitopuncture was put on the acupoints when performing the task for one hour in A-group. In this study HRV exhibited a significant difference between the two groups after the simulating task (P < 0.05). A conclusion that magnitopuncture stimuli can reduce the driver mental stress and fatigue effectively was drawn.
Acupuncture Points ; Adult ; Automobile Driving ; Autonomic Nervous System ; physiopathology ; Electric Stimulation Therapy ; methods ; Fatigue ; therapy ; Heart Rate ; physiology ; Humans ; Magnetics ; therapeutic use ; Male ; Stress, Physiological ; therapy ; Vibration ; adverse effects