Humans ; Occupational Diseases ; diagnosis ; Saliva ; chemistry ; metabolism ; Stress, Psychological ; diagnosis

Calibration ; Chemistry Techniques, Analytical ; methods ; Humans ; Lead ; blood

OBJECTIVETo investigate the relationship between emotion and plasma fibrinogen level in train crew.

METHODSA cross-sectional study was conducted in 350 male workers of passenger car, freight car, shunting locomotive for passenger service, and high-speed car from a locomotive depot. The factors involved in this study were as follows: common factors including educational level, physical exercise, smoking, drinking, and physical environment, emotional/characteristic factors including sleep quality, depression, and coping strategies, and working -related factors including effort -reward imbalance, working age, responsibility for people, responsibility for work, intragroup conflicts, and intergroup conflicts. Variables were measured with common rating scales at home and abroad. Plasma fibrinogen level was determined by immunological turbidimetry. The Spearman rank correlation test and multiple stepwise regression were used to analyse the influential factors for fibrinogen level. The original concentration of fibrinogen was subjected to logarithmic transformation in statistical analysis.

RESULTSThe Spearman rank correlation test showed that plasma fibrinogen level was significantly correlated with age(r = 0.228, P = 0.001), working age(r = 0.231, P = 0.001), and emotion (r = -0.138, P = 0.016), but showed no significant relationship with other variables (P > 0.05). The multiple stepwise regression with fibrinogen level as a dependent variable was performed in four models. Model 1 showed that emotion and age were included in the regression after adjustment for common factors (P < 0.05). Model 2 also showed inclusion of emotion and age in the regression after adjustment for common factors and occupational stress factors (P < 0.05). Model 3 showed inclusion of emotion in the regression after adjustment for common factors, occupational stress factors, and psychological factors (P < 0.05). Model 4 showed inclusion of emotion and age after adjustment for common factors, occupational stress factors, psychological factors, and relief factors (P < 0.05).

CONCLUSIONThe emotion of train crew is correlated with plasma fibrinogen level.

Adult ; Cross-Sectional Studies ; Emotions ; Fibrinogen ; analysis ; Humans ; Male ; Middle Aged ; Occupational Diseases ; blood ; Plasma ; chemistry ; Railroads ; Risk Factors ; Stress, Psychological ; blood ; Surveys and Questionnaires

OBJECTIVETo establish a method for simultaneously determining vanilmandelic acid (VMA), 5-hydroxyindoleacetic (5-HIAA), 3, 4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in urine by high-performance liquid chromatography (HPLC).

METHODSAfter being filtered with a 0.45 µm membrane syringe filter, the urinary samples were injected directly into the HPLC system using a C18 chromatographic column and a fluorescence detector. The excitation and emission wavelengths were chose as 280 nm and 315 nm, respectively, and the urinary samples were carried with a mobile phase of methanol-0.1 mol/L phosphate buffered solution (V/V = 20:80) at a flow rate of 1.0 ml/min and an injection volume of 20 µl.

RESULTSUsing the method reported here, the correlation coefficients of VMA, 5-HIAA, DOPAC, and HVA were 0.9999, 0.9998, 0.9997, 0.9999, respectively, over linear ranges of 0-2.5, 0-2.0, 0-2.0, and 0-2.5 µg/ml, the minimum detectable concentrations were 0.006, 0.008, 0.012, and 0.0082 µg/ml, the average precisions were 4.2%, 3.7%, 4.9%, and 3.6%, and the recovery rates were 91%∼102%, 93%∼101%, 94%∼101%, and 89%∼ 102%.

CONCLUSIONThis determination method is simple, efficient, accurate, and sensitive for the simultaneous detection of VMA, 5-HIAA, DOPAC, and HVA in urine.

Biogenic Amines ; urine ; Chromatography, High Pressure Liquid ; Homovanillic Acid ; urine ; Humans ; Hydroxyindoleacetic Acid ; urine ; Vanilmandelic Acid ; urine

OBJECTIVETo explore the possibility for 17-OHCS, 17-KS, VMA and HVA in urine as indicators of occupational stress identification and evaluation.

METHODS225 policies were investigated by using convenience sampling and cluster sampling methods, occupational stressors, strains, buffer factors and individual factors were measured by questionnaires. The urine 17-OHCS, 17-KS, VMA and HVA level was tested by ELISA and high performance liquid chromatography method respectively.

RESULTSThe urine 17-OHCS level of the groups with high coworker support and job control scores was lower than that of the groups with low score group (P < 0.05), the urine 17-OHCS level of the group with high negative affectivity score was higher than that of the group with low score group (P < 0.05). The urine 17-KS level of the group with high job security score was lower than that of the counterparts (P < 0.05). The urine VMA level of the group with high coworker support score was lower than that of the counterparts (P < 0.05). The urine HVA level of the group with high coping strategy score was lower than that of counterpart (P < 0.05). Logistic regression analysis revealed that the job control and respect were the protective factors of urine 17-OHCS level increase (OR = 0.353 and 0.352 respectively), but over-commitment, self-esteem and daily hassles were the risk factors of urine 17-OHCS with level increase (OR = 3.391, 2.439, and 2.584 respectively). The risk of urine 17-KS level increase for the groups high job control and job security scores were respectively 0.387, 0.356 times than that of the counterparts, the risk of urine VMA level increase for the group with high negative affectivity score was 2.643 times than that of the counterparts.

CONCLUSIONThe urine 17-OHCS, 17-KS and VMA level were potential indicators for occupational stress evaluation.

Adaptation, Psychological ; Adrenal Cortex Hormones ; urine ; Adult ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases ; urine ; Risk Factors ; Stress, Psychological ; urine ; Surveys and Questionnaires ; Young Adult

OBJECTIVETo explore the relationship of occupational stressors with the serum levels of triiodothyronine (T3), thyroxine (T4), and thyroid stimulating hormone (TSH).

METHODSUsing convenience sampling and cluster sampling methods, 225 policemen from a local police station in China were enrolled as subjects. Questionnaires were used to investigate demographic features and occupational stressors in those subjects. The serum levels of T3, T4, and TSH were measured by radioimmunoassay. The SPSS 13.0 software was used to perform t test or analysis of variance, partial correlation analysis, and multivariate non-conditional logistic regression analysis.

RESULTSReward was positively correlated with the level of T3(P<0.05). Daily tension was positively correlated with the level of T4(P<0.05). Psychological demand, effort, and daily tension were negatively correlated with the level of TSH (all P<0.05). The quality of sleep was positively correlated with the level of TSH (P<0.05). The logistic regression analysis revealed that the risk of increase in T3 level in the group with a high score for daily tension was 3.19-fold higher than that in the group with a low score, while the risk of increase in T3 level in the group with a high score for negative emotion was 1.32-fold higher than that in the group with a low score. The risk of increase in TSH level in the group with a high score for negative emotion was 0.43-fold that in the group with a low score.

CONCLUSIONThe occupational stressors are correlated with the serum levels of thyroid hormones. Occupational stress can result in an increase in T3 level and a decrease in TSH level. However, occupational stress has no effect on T4 level.

China ; Humans ; Logistic Models ; Multivariate Analysis ; Police ; Stress, Psychological ; Surveys and Questionnaires ; Thyrotropin ; blood ; Thyroxine ; blood ; Triiodothyronine ; blood