4.Analyses on relevant factors of the prognosis of patients with acute organophosphate poisoning.
Xin KE ; Shaoce ZHI ; Dan ZHENG ; Guangling HONG ; Guangju ZHAO ; Mengfang LI ; Qiaomen QIU ; Bing WU ; Zhongqiu LU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(3):186-189
OBJECTIVETo find out a method which can assess the prognosis of patients with Acute Organophosphate Poisoning objectively and increase the successful ratio of treatment by investigating relevant factors on the prognosis of the patients with Acute Organophosphate Poisoning.
METHODSWe retrospected 116 patients with Acute Organophosphate Poisoning who were treated in our hospital's emergency room from April 2006 to March 2014. According to the outcome of patients, we distributed the patients to death group and survival group, compared the clinic data and using multivariate analysis with Logistic regression to prognosis factors.
RESULTS116 cases of acute organophosphate poisoning patients died in 23 cases, improved in 93 cases. Death group patients' APACHE-II score are higher than whose in the survival group (P < 0.05). Compared with the survival group, patients' body temperature, blood pressure, pH, GCS index were lower in the death group (P < 0.05) and Cr, WBC, ALT, AST, CK-MB, blood glucose, blood lactic acid, heart rate were higher in the death group (P < 0.05), there were significant difference between two groups with statistical.Low blood pressure, lower GCS score, hyperglycemia and high white blood cell count, were independent risk factors of poor prognosis, and hypotension was maximum value of all the factor (OR = 54.22).
CONCLUSIONAPACHE II prognostic scoring system can be accurately response, vital signs, white blood cell count, pH, serum creatinine, GCS score and serum sodium value which in this system may be associated with prognosis. To evaluate the severity and prognosis of illness Blood glucose, ALT, AST, CK-MB's rising also has certain value.
APACHE ; Acute Disease ; Blood Glucose ; Humans ; Leukocyte Count ; Logistic Models ; Multivariate Analysis ; Organophosphate Poisoning ; Prognosis ; Retrospective Studies ; Risk Factors
5.Protective effect of ghrelin against paraquat-induced acute lung injury in mice.
Yao LIU ; Ruijuan GUO ; Fei WANG ; Guangju ZHAO ; Zhongqiu LU ; Qiaomeng QIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2014;32(3):190-194
OBJECTIVETo measure the levels of ghrelin-induced expression or activation of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and
NAD(P)Hquinone oxidoreductase 1 (NQO1) in the PQ-injured lungs of mice and to evaluate the protective effect of ghrelin against paraquat (PQ)-induced acute lung injury in mice.
METHODSAccording to the random number table method, 50 ICR mice of clean grade were assigned to 5 groups: normal control group (n = 10), PQ group (n = 10), and ghrelin intervention groups (n = 30). For PQ group, mice were injected with a single dose of PQ (20 mg/kg, i.p.); for ghrelin intervention groups, mice were injected with a single dose of PQ (20 mg/kg, i.p.), and then ghrelin was injected at three concentrations (16.58, 33.15, and 49.73 µg/kg). Lung tissues were collected and proceeded to the following studies. HE staining was used for histopathological examination under a light microscope, and the changes in nuclear expression of Nrf2 were evaluated by Western blot. The activities of HO-1 and NQO1 were measured by ELISA. Malondialdehyde (MDA) content and MPO activity were measured by colorimetry. Another 40 mice were divided into PQ group (n = 10) and 16.58, 33.15, and 49.73 µg/kg ghrelin intervention groups (n = 10 for each); mortality and clinical manifestations were recorded within 72 h.
RESULTSCompared with the normal control group, the PQ group showed significant increases in nuclear protein level of Nrf2, content of MDA, and activities of HO-1, NQO1, and MPO (P < 0.05 for all). Compared with the PQ group, ghrelin treatment significantly increased the expression of Nrf2 and activities of HO-1 and NQO1 and significantly reduced the content of MDA and activity of MPO (P < 0.01 for all). Histopathological studies indicated that ghrelin showed an antioxidant property that reduced the histological changes induced by PQ in the lungs. The ghrelin intervention groups had a significantly lower mortality than the PQ group, and there was a significant difference between the high-dose ghrelin intervention group and PQ group (P < 0.05).
CONCLUSIONGhrelin can up-regulate nuclear expression of Nrf2, increase the activities of HO-1 and NQO1, and reduce the activity of MPO and content of MDA, thus protecting PQ-exposed mice from acute lung injury.
Acute Lung Injury ; chemically induced ; metabolism ; Animals ; Ghrelin ; pharmacology ; Heme Oxygenase-1 ; metabolism ; Lung ; drug effects ; metabolism ; Male ; Malondialdehyde ; metabolism ; Membrane Proteins ; metabolism ; Mice ; Mice, Inbred ICR ; NAD(P)H Dehydrogenase (Quinone) ; metabolism ; NF-E2-Related Factor 2 ; metabolism ; Oxidative Stress ; Paraquat ; poisoning ; Peroxidase ; metabolism
6.Hemoperfusion alleviated paraquat-induced kidney inflammation injury of rabbit.
Shaoce ZHI ; Dong WU ; Lingwen LI ; Guangliang HONG ; Mengfang LI ; Bin WU ; Zhongqiu LU ; E-mail: LZQ640815@163.COM.
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(9):658-662
OBJECTIVETo investigate the effect of hemoperfusion on paraquat-Induced kidney inflammation injury of rabbit and the mechanism of it.
METHODS60 male rabbits were randomly divided into 4 groups, the normal control group (n=6, the rabbits were given NS by gavage) , blank control group (n=18, he rabbits were given 2 hours hemoperfusion once within 1 hour after given NS by gavage), paraquat poisoning group (n=18, the rabbits were given 50 mg/kg 20% paraquat solution by gavage) , hemoperfusion treatment group (n=18, the rabbits were given 2 hours hemoperfusion once within 1 hour after 20% paraquat solution espoused). The last 3 groups were divided into 3 observation time groups (1, 3, 7 day), contained 6 rabbits each group. On days 1, 3, 7 all groups rabbits were anesthetized and sacrificed, and their kidney tissues collected. The levels of NF-κB mRNA by RT-PCR, and the expression of NF-κB protein was measured by Western blotting,The expression levels of TNF-α, IL-6, iNOS measured by chemical colorimetric method to to observe inflammatory injury.
RESULTSCompared with the normal control group rabbits, there were no changes in the TNF-α, IL-6, iNOS, NF-κB mRNA and protein of blank control group (P>0.05), while the expression of TNF-α, IL-6, NF-κB mRNA and protein in the kidney tissue of PQ group and were significantly increased (P<0.05). The pathological results of kidney tissues were no abnormalities onnormal control group and blank control group.
CONCLUSIONHP significantly increase resistance to PQ-induced inflammation injury in the rabbit kidney and exert a protective effect on PQ-induced kidney injury.
Animals ; Hemoperfusion ; Inflammation ; chemically induced ; therapy ; Interleukin-6 ; metabolism ; Kidney ; drug effects ; physiopathology ; Male ; NF-kappa B ; metabolism ; Nitric Oxide Synthase Type II ; metabolism ; Paraquat ; toxicity ; RNA, Messenger ; metabolism ; Rabbits ; Tumor Necrosis Factor-alpha ; metabolism