No abstract available.
E-Selectin* ; Humans ; P-Selectin*

BACKGROUND: Since the management of atopic dermatitis often needs prolonged administration of medication, the laboratory index reflecting disease severity is necessary for optimal treatment for this disease. OBJECTIVES: The purpose of this study is to evaluate peripheral eosinophil counts, serum total IgE, eosinophilic cationic protein(ECP), IL-4 and soluble E-selectin as severity indices or disease marker. METHOD: A total of 21 patients with atopic dermatitis and 21 normal controls were evaluated for the symptoms and signs according to SCORAD index and measured for peripheral eosinophil counts, serum total IgE, ECP, IL-4 and soluble E-selectin. RESULTS: Peripheral eosinophil counts, serum total IgE, ECP and soluble E-selectin levels of patients with atopic dermatitis were significantly increased compared with those of normal control(p<0.05). Moreover, serum ECP and soluble E-selectin correlated with SCORAD score. Serum IL-4 levels of patients with atopic dermatitis were slightly increased compared with those of normal control but not statistically significant(p>0.05). CONCLUSION: ECP and soluble E-selectin were good serum marker reflecting the severity of atopic dermatitis.
Biomarkers ; Dermatitis, Atopic* ; E-Selectin* ; Eosinophil Cationic Protein* ; Eosinophils* ; Humans ; Immunoglobulin E* ; Interleukin-4*

PURPOSE: Augmentation in the expression of E-selectin on activated vascular endothelial cells regulates leukocyte migration into the tissue. These molecules are also shed into the circulation as a soluble form. The level of soluble forms in the serum has been known to be correlated with those expressed on the endothelial cells, thus it can be used as a marker of inflammation in that tissue. The purpose of this study is to compare the serum levels of soluble E-selectin(sE-selectin) in patients with atopic dermatitis(AD), atopic asthmatics, and healthy non-atopics, and to determine whether sE-selectin levels are correlated with disease activity in patients with atopic dermatitis. METHODS: We examined serum sE-selectin levels, serum total IgE levels, and total eosinophil counts from 18 children with AD, 15 atopic asthmatics and 15 healthy non-atopics. The severity of AD was assessed by clinical scoring(SCORAD index). We compared the sE-selectin levels among the three groups and investigated the correlations with SCORAD index. RESULTS: The children with AD had significantly higher levels of sE-selectin than those of atopic asthmatics(P<0.05) and of healthy non-atopics(P<0.05). There was no difference in the serum sE-selectin levels between the groups of atopic asthmatics and healthy non- atopics. Serum sE-selectin levels were correlated with SCORAD index in patients with AD, (P<0.05) but not significantly correlated with serum total IgE levels and total eosinophil counts. There were no significant correlations among SCORAD index, serum total IgE levels, and total eosinophil counts. CONCLUSION: The serum sE-selectin level is elevated only in patients with AD, not in atopic asthmatics. Therefore, sE-selectin could be considered as a useful marker of the disease activity in AD.
Biomarkers* ; Child ; Dermatitis, Atopic* ; E-Selectin* ; Endothelial Cells ; Eosinophils ; Humans ; Immunoglobulin E ; Inflammation ; Leukocytes

OBJECTIVETo investigate the changes of adhesion molecules, cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate (cGMP) in divers post 480 heliox saturation diving.

METHODSFour divers were compressed within 96 hours to depths of 480 m with heliox-oxygen and held at the designated depth for 49 hours, excursion to 493 m during their saturation stay, then decompressed within 302 hours to the surface. The blood samples were collected before compression and post decompression, the expression level of intercellular adhesion molecule-1(ICAM-1), E-selectin, P-selectin, cAMP, cGMP were detected with ELISA analysis box.

RESULTSCompared with the levels of CAMs before compression, the levels of ICAM-1, E-selectin, P-selectin and cGMP in the serum were changed post decompression (P > 0.05). The levels of cAMP were significantly elevated post decompression (629.91 +/- 75.01) nmol/L vs (66.72 +/- 83.15) (P < 0.05).

CONCLUSIONThe decompression schedule in this heliox saturation diving is safe, the decompression sickness pathology in this diving has not been induced. But the stress response of divers are enhanced by this great depth saturation diving.

Cyclic AMP ; blood ; Diving ; physiology ; E-Selectin ; blood ; Helium ; Humans ; Intercellular Adhesion Molecule-1 ; blood ; Oxygen ; P-Selectin ; blood

BACKGROUND: Cyclosporine effectively suppresses immune responses and inhibits skin homing T cell responses in psoriasis. E-selectin is known to be up-regulated on vascular endothelium of inflammatory skin lesions such as psoriasis. PURPOSE: Based on our previous study that cyclosporine decreased lesional cutaneous lymphocyte antigen (CLA)+ T cells in psoriatic patients, we tried to find any change of CLA+ T cells in peripheral blood in psoriatic patients, since psoriasis is a disease of systemic T cell activation. Subjects and Methods: Peripheral blood of 8 patients with chronic plaque type psoriasis at 0, 3, 6, 12, 18 weeks after cyclosporine was examined by flow cytometry using anti-CLA antibody. Five skin biopsy samples at 0, 3, 6, 12, 18 weeks were immunohistochemically stained with anti E-selectin antibody. RESULTS: Our results demonstrate that the number of CD3+ CLA+ and CD4+CLA+ T cells was significantly reduced in the peripheral blood at week 3, but gradually increased to the level of baseline at 18 weeks. In psoriatic skin lesions, with decrease of PASI score and CLA+ T cells number, the expression of E-selectin on the endothelial cells was gradually decreased throughout 18 weeks of therapy. CONCLUSION: These results suggest that cyclosporine suppresses the migration of skin homing T cells to psoriatic skin lesions, in part, through the inhibition of E-selectin on the endothelial cells.
Biopsy ; Cyclosporine* ; E-Selectin ; Endothelial Cells ; Endothelium, Vascular ; Flow Cytometry ; Humans ; Lymphocytes ; Psoriasis ; Skin* ; T-Lymphocytes

BACKGROUND AND OBJECTIVES: Blood levels of soluble CD30 and soluble E-selectin are related to allergic diseases such as atopic dermatitis or asthma. However, these levels have not been well investigated in allergic rhinitis. We evaluated the association of blood concentrations of these cytokines in allergic rhinitis. MATERIALS AND METHODS: The concentrations of soluble CD30 and soluble E-selectin in blood were analyzed by enzyme linked immunosorbant assay. The levels of these cytokines were compared between allergic rhinitis patients (n=48) and age-matched healthy controls (n=27), and a relationship was found with symptom severity. RESULT: The concentration of soluble CD30 was significantly increased in allergic rhinitis patients compared to that in normal healthy people (p=0.042). However, soluble E-selectin was not significantly increased in allergic rhinitis patients (p>0.05). CONCLUSION: These results suggest a possible role of soluble CD30 in the blood of allergic rhinitis.
Asthma ; Cytokines ; Dermatitis, Atopic ; E-Selectin ; Humans ; Rhinitis ; Rhinitis, Allergic, Perennial

BACKGROUND: Psoriasis is a chronic skin disease characterized histologically by prominent keratinocyte hyperplasia and an early inflammatory cell infiltrate. However, the pathogenesis is not fully understood yet. Recently, the evidence that T lymphocytes play a key role in the pathogenesis of psoriasis is compelling. OBJECTIVE: The purpose of this study is to evaluate serum soluble E-selectin, MCP-1 and RANTES as severity indices or disease marker. In this study, we investigated a possible correlation between disease activity in psoriasis and serum soluble E-selectin, MCP-1 and RANTES, respectively. METHODS: Fifteen patients with psoriasis and fifteen normal controls were included in this study. The patients were evaluated for the symptoms and signs according to PASI score. We measured soluble E-selectin, MCP-1 and RANTES levels with blood samples drawn from patients and normal controls. RESULTS: 1. Soluble E-selectin serum levels were significantly increased in psoriatic patients compared with normal controls (p<0.05). Moreover, a significant correlation was demonstrated between serum soluble E-selectin values and PASI scores (r=0.54, p<0.05). 2. Serum MCP-1 levels were significantly increased in psoriatic patients compared with normal conrols (p<0.05). However, no significant correlation was observed between MCP-1 values and PASI scores (r=-0.12). 3. Serum RANTES levels were significantly increased in psoriatic patients compared with normal controls (p<0.05). Moreover, a positive correlation was observed between serum RANTES levels and PASI scores (r=0.63, p<0.05). CONCLUSION: These results suggest that serum levels of soluble E-selectin and RANTES could be used as marker of the disease activity in psoriatic patients.
Chemokine CCL5* ; E-Selectin* ; Humans ; Hyperplasia ; Keratinocytes ; Psoriasis* ; Skin Diseases ; T-Lymphocytes

OBJECTIVETo investigate the effect of E-selectin A561C (S128R) polymorphism on blood pressure.

METHODS347 essential hypertensive patients (male 163 and female 184, age 46.08 +/- 12.49 y, and body mass index 26.13 +/- 3.14 kg/m(2)) and 315 normal controls (male 93 and female 222, age 42.21 +/- 14.67 y, and body mass index 25.66 +/- 3.19 kg/m(2)) were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Phenotypic measurements included blood pressure, blood glucose, serum triglycerides, serum total, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol, serum uric acid, blood urea nitrogen, nitric oxide, endothelin, angiotensin I, and II and plasma aldosterone.

RESULTSThe frequencies of the AC and CC genotypes (6.92%) and C allele (4.76%) were significantly (P = 0.029 and 0.013) higher in hypertensive patients than normal controls (3.17% and 2.22%). The relative risk of hypertension associated with the C allele was 2.197 (95% CI: 1.164-4.144). Both diastolic blood pressure and mean arterial pressure were higher in C allele carriers than AA subjects (P = 0.049 and 0.024). Furthermore, C allele carriers, compared with AA subjects, had higher concentrations of blood glucose, and total and LDL cholesterol (P = 0.031, 0.046, and 0.003) and lower concentrations of nitric oxide (P = 0.036).

CONCLUSIONThe E-selectin A561C (S128R) polymorphism might affect blood pressure in Chinese.

Adult ; E-Selectin ; genetics ; Female ; Genotype ; Humans ; Hypertension ; genetics ; Male ; Middle Aged ; Polymorphism, Single Nucleotide

BACKGROUNDS: Recent experimental studies demonstrate massive leukocytes extravasation at sites of cerebral ischemia even with the first hours of disease. Leukocytes are now considered to potentiate ischemic neural damage by microvasculature obstruction and generation of neurotoxic substances. Adhesion molecules mediate adhesion between endothelial cells and leukocytes as a precondition for extravasation of leukocytes at sites of tissue injury. We conducted a prospective study to investigate the serum level of ICAM-1, P-selectin, and E-selectin in patients with acute ischemic stroke, and with atherosclerosis. METHODS: Serum was sampled from patients within 24 hrs of acute ischemic stroke(n=20), from patients with previous (> 1 month) transient or persistent ischemic neurologic deficit associated with atherosclerosis(n=22), and control patients without a history of vascular disease(n=20). Concentrations of soluble ICAM-1(sICAM-1), P-selectin(sP-selectin), and E-electin(sE-selectin) were measured by enzyme-linked immunosorbent assay(ELISA). RESULTS: Compared with control subjects, sICAM-1 and sE-selectin were significantly elevated in patients with acute ischemic stroke and in previous symptomatic atherosclerosis(p=0.0001 and p=0.004). The serum level of sP-selectin in patients with acute ischemic stroke was higher than that in patients with previous symptomatic atherosclerosis and control subjects(p=0.0004). CONCLUSIONS: The results suggest a chronic elevation of ICAM-1 and E-selectin in patients with previous symptomatic atherosclerosis and also acute changes of them in patients with acute ischemic stroke. These findings indicate that acute changes of serum P-selectin occurred in response to acute ischemic stroke.
Atherosclerosis ; Brain Ischemia ; Cell Adhesion Molecules ; E-Selectin ; Endothelial Cells ; Humans ; Intercellular Adhesion Molecule-1 ; Leukocytes ; Microvessels ; Neurologic Manifestations ; P-Selectin ; Prospective Studies ; Stroke*

BACKGROUNDAdhesion molecules play an important role in the development and progression of coronary atherosclerosis. The aim of this study was to compare concentrations of soluble forms of adhesion molecules in patients with different clinical presentations of coronary artery disease (CAD).

METHODSOne hundred and twenty-eight patients with CAD were divided into three groups; the first group was acute myocardial infarction group (AMI group, n = 45), the second group was unstable angina pectoris group (UAP group, n = 48), the third group was stable angina pectoris group (SAP group, n = 35). We compared them with patients with normal coronary arteries (control group, n = 31). The serum levels of vascular cell adhesion molecule (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), E-selectin and P-selectin were measured in all subjects.

RESULTSThe serum level of VCAM-1 in the AMI group was significantly higher than in the UAP, SAP and control groups (P < 0.01). The level in the UAP group was significantly higher than the SAP group and control group (P < 0.01) and the level in the SAP group was significantly higher than in the control group (P < 0.01). The serum ICAM-1 level was significantly elevated in the AMI, UAP and SAP groups as compared to the control group (P < 0.01). The levels of serum E-selectin and P-selectin in the AMI and UAP groups were significantly higher than in the SAP and control groups (P < 0.01).

CONCLUSIONSIncreased levels of VCAM-1 and ICAM-1, E-selectin and P-selectin, as markers of inflammation, showed the importance of inflammatory processes in the development of atherosclerosis and clinical expression of CAD. Soluble ICAM-1, VCAM-1, E-selectin and P-selectin concentrations are useful indicators of the presence of atherosclerosis and the severity of CAD clinical presentation.

Coronary Artery Disease ; blood ; pathology ; E-Selectin ; blood ; Female ; Humans ; Intercellular Adhesion Molecule-1 ; blood ; Male ; Middle Aged ; P-Selectin ; blood ; Vascular Cell Adhesion Molecule-1 ; blood