1.Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43.
Hyun Kyoung LEE ; Byoung Hee LEE ; Seung Hyeok SEOK ; Min Won BAEK ; Hui Young LEE ; Dong Jae KIM ; Yi Rang NA ; Kyoung Jin NOH ; Sung Hoon PARK ; Dutta Noton KUMAR ; Hiroaki KARIWA ; Mina NAKAUCHI ; Suk Jin HEO ; Jae Hak PARK
Journal of Veterinary Science 2010;11(2):165-167
Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.
Antibodies, Viral/*immunology
;
Blotting, Western
;
Coronavirus 229E, Human/*immunology
;
Coronavirus OC43, Human/*immunology
;
Cross Reactions
;
Humans
;
Nucleocapsid Proteins/genetics/*immunology
;
Recombinant Proteins/immunology
;
SARS Virus/genetics/*immunology
;
Severe Acute Respiratory Syndrome/diagnosis/*immunology
2.NaCl plus chitosan as a dietary salt to prevent the development of hypertension in spontaneously hypertensive rats.
Sung Hoon PARK ; Noton Kumar DUTTA ; Min Won BAEK ; Dong Jae KIM ; Yi Rang NA ; Seung Hyeok SEOK ; Byoung Hee LEE ; Ji Eun CHO ; Geon Sik CHO ; Jae Hak PARK
Journal of Veterinary Science 2009;10(2):141-146
The effect of NaCl plus 3% chitosan on the systolic blood pressure of spontaneously hypertensive rats (SHR) were evaluated and compared with NaCl plus KCl (NaCl, 49.36% + KCl 49.36%) and chitosan or NaCl treatment alone. In SHR, administration of NaCl plus chitosan (44 mM Na/day) for two months significantly decreased the systolic blood pressure greater than of NaCl plus KCl and NaCl alone. NaCl plus chitosan resulted, though not statistically significant, in decreased urinary Na+ excretion and decreased blood urea nitrogen levels. Urinary creatinine of NaCl plus chitosan was slightly decreased compared to 3 treated groups. Serum electrolytes levels, however, remained unchanged. The combination of NaCl and chitosan may be superior to the conventional use of NaCl plus KCl or NaCl alone in the prevention of hypertension. Even though these supplementary diets have demonstrated potential anti-hypertensive effects in the experimental animal model, further research is needed before any recommendations can be made.
Angiotensin I/blood
;
Angiotensin II/biosynthesis
;
Animals
;
Blood Pressure/*drug effects/physiology
;
Blood Urea Nitrogen
;
Body Weight/drug effects
;
Chitosan/*administration & dosage
;
Chlorides/blood/urine
;
Creatinine/urine
;
Heart/physiology
;
Histocytochemistry
;
Hypertension/*prevention & control
;
Kidney/physiology
;
Male
;
Potassium/blood/urine
;
Potassium Chloride/administration & dosage
;
Random Allocation
;
Rats
;
Rats, Inbred SHR
;
Sodium/blood/urine
;
Sodium Chloride, Dietary/*administration & dosage
;
Systole/drug effects/physiology