Objective To investigate the influence of the serum vascular endothelial growth factor (VEGF) in the glioma patients by the malignant degress.Methods Quantitative analysis the serum VEGF levels with the double antibody sandwich assay (ELISA) for 35 cases of glioma patients and 10 cases of healthy human and analyze the correlation of them.Results Mean serum VEGF expression in glioma patients was 120.71 ±45.99 pg/ml,normal value of the healthy people was 63.70 ± 6.50,the difference was statistically significant (P ＜0.05); serum VEGF expression was 156.43 ± 14.90 pg/ml in high-grade glioma patients (Ⅲ-Ⅳ grade) and 67.14 ± 6.12 pg/ml in low-grade glioma patients (Ⅰ-Ⅱ grade),the difference was statistically significant (P ＜ 0.05) ; there' s no different from low-grade glioma patients (Ⅰ-Ⅱ grade) and nomal(P ＞ 0.05).Conclusion The serum VEGF levels in the glioma patients was influence by the malignant degress.