Objective To analyze the expression of microRNA-451(miR-451)in children with microcytic anemia and chronic infectious anemia,and to explore the correlation between miR-451 and erythrocyte-related parameters and its diagnostic value for anemia in children.Methods The expres-sion levels of miR-451 in serum were detected in children with microcytic anemia(n=80),chronic infectious anemia(n=80),and healthy controls(n=80)using real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The correlation between serum miR-451 and erythrocyte-re-lated parameters[red blood cell distribution width(RDW),red blood cells(RBC),mean corpuscu-lar hemoglobin concentration(MCHC),hemoglobin(Hb),mean corpuscular hemoglobin content(MCH),mean corpuscular volume(MCV)]was analyzed using Pearson correlation analysis.The re-ceiver operating characteristic(ROC)curve was plotted to analyze the diagnostic value of miR-451 for microcytic anemia,chronic infectious anemia,and the severity of anemia.Multivariate Logistic regression analysis was used to investigate the influencing factors for the occurrence of microcytic a-nemia and chronic infectious anemia in children.Results The expression levels of serum miR-451 in the microcytic anemia group and chronic infectious anemia group were lower than those in the healthy control group(P＜0.05).The miR-451 was negatively correlated with RDW(r=-0.388,P＜0.05),and positively correlated with Hb,RBC,MCHC,MCH,and MCV(r=0.402,0.393,0.391,0.360,0.323,P＜0.05).ROC curve analysis showed that the areas under the curve of serum miR-451 for diagnosing microcytic anemia and chronic infectious anemia was 0.718 and 0.735,respectively,which was higher than that of erythrocyte-related parameters such as RDW,Hb,RBC,MCHC,MCH,and MCV(P＜0.05).Multivariate Logistic regression analysis showed that body mass index,RDW,Hb,RBC,MCHC,MCH,MCV,and miR-451 were influencing fac-tors for the occurrence of microcytic anemia and chronic infectious anemia in children(P＜0.05).With the aggravation of anemia in children,the expression level of serum miR-451 gradually de-creased(P＜0.05).ROC curve analysis showed that the level of serum miR-451 had a high diag-nostic value for the severity of anemia in children.Conclusion The expression of miR-451 is re-duced in serum of children with microcytic anemia and chronic infectious anemia,and its expression level is correlated with erythrocyte-related parameter levels.Serum miR-451 has a high diagnostic value for microcytic anemia,chronic infectious anemia,and the severity of anemia.

Objective To analyze the expression of microRNA-451(miR-451)in children with microcytic anemia and chronic infectious anemia,and to explore the correlation between miR-451 and erythrocyte-related parameters and its diagnostic value for anemia in children.Methods The expres-sion levels of miR-451 in serum were detected in children with microcytic anemia(n=80),chronic infectious anemia(n=80),and healthy controls(n=80)using real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The correlation between serum miR-451 and erythrocyte-re-lated parameters[red blood cell distribution width(RDW),red blood cells(RBC),mean corpuscu-lar hemoglobin concentration(MCHC),hemoglobin(Hb),mean corpuscular hemoglobin content(MCH),mean corpuscular volume(MCV)]was analyzed using Pearson correlation analysis.The re-ceiver operating characteristic(ROC)curve was plotted to analyze the diagnostic value of miR-451 for microcytic anemia,chronic infectious anemia,and the severity of anemia.Multivariate Logistic regression analysis was used to investigate the influencing factors for the occurrence of microcytic a-nemia and chronic infectious anemia in children.Results The expression levels of serum miR-451 in the microcytic anemia group and chronic infectious anemia group were lower than those in the healthy control group(P＜0.05).The miR-451 was negatively correlated with RDW(r=-0.388,P＜0.05),and positively correlated with Hb,RBC,MCHC,MCH,and MCV(r=0.402,0.393,0.391,0.360,0.323,P＜0.05).ROC curve analysis showed that the areas under the curve of serum miR-451 for diagnosing microcytic anemia and chronic infectious anemia was 0.718 and 0.735,respectively,which was higher than that of erythrocyte-related parameters such as RDW,Hb,RBC,MCHC,MCH,and MCV(P＜0.05).Multivariate Logistic regression analysis showed that body mass index,RDW,Hb,RBC,MCHC,MCH,MCV,and miR-451 were influencing fac-tors for the occurrence of microcytic anemia and chronic infectious anemia in children(P＜0.05).With the aggravation of anemia in children,the expression level of serum miR-451 gradually de-creased(P＜0.05).ROC curve analysis showed that the level of serum miR-451 had a high diag-nostic value for the severity of anemia in children.Conclusion The expression of miR-451 is re-duced in serum of children with microcytic anemia and chronic infectious anemia,and its expression level is correlated with erythrocyte-related parameter levels.Serum miR-451 has a high diagnostic value for microcytic anemia,chronic infectious anemia,and the severity of anemia.

Objective:By establishing the reference intervals of free thyroxine (FT 4), thyroid stimulating hormone (TSH) and thyroid peroxidase antibody (TPOAb) of pregnant women in different pregnancy in Yangzhou, and analyzing the dynamic trends of each indicator, so as to provide a basis for timely and accurate diagnosis of thyroid disease during pregnancy, and promote prenatal and postnatal care. Methods:Clinical data of 3 726 healthy early (1 747 cases), middle (1 481 cases), and late (498 cases) pregnant women were collected from the Department of Perinatal and Health Care in Yangzhou Women and Children Hospital, the Affiliated Hospital of Yangzhou University Medical College from October 2017 to October 2018. At the same time, data of 407 non-pregnant women in the same period were collected as normal controls. The levels of serum FT 4, TSH and TPOAb in each stage of pregnancy were detected by Beckman automatic chemiluminescence analyzer. The reference interval was established by using the 95% reference value of the bilateral limit, and the differences of early, middle and late pregnancy were compared. Results:There were significant differences in FT 4 levels between early, middle and late stages of pregnancy ( H = 82.56, P < 0.01), with the early stage higher than the middle stage ( P < 0.01) and the middle stage higher than the late stage( P < 0.01). The difference of TSH levels was statistically significant ( H = 91.27, P < 0.01), in which the early stage was lower than the middle stage ( P < 0.01), and the middle stage was lower than the late stage ( P < 0.01). There was a statistically significant difference in TPOAb levels ( H = 30.36, P < 0.01). There was no significant difference between the early stage and the middle stage ( P > 0.05), and the early and middle stages were higher than the late stage ( P < 0.01). The reference intervals of thyroid function index in different pregnancies were, early pregnancy: FT 4 8.28 - 15.66 pmol/L, TSH 0.11 - 4.23 mU/L, TPOAb 0.10 - 16.46 U/ml; middle pregnancy: FT 4 7.38 - 14.36 pmol/L, TSH 0.13 - 4.67 mU/L, TPOAb 0.10 - 18.97 U/ml; and late pregnancy: FT 4 6.33 - 11.39 pmol/L, TSH 0.40 - 3.96 mU/L, TPOAb 0.10 - 6.17 U/ml. Conclusions:There are significant differences in serum thyroid function indicators in different pregnant women. Establishing reference intervals of thyroid function indicators in different stages of pregnancy have important clinical significance for diagnosis of thyroid disease and eugenics.

AIM:To investigate the role of Ywhab in the growth of mouse B-cell lymphoma,and to explore the potential underlying mechanisms.METHODS:The correlation between Ywhab and human diffuse large B-cell lymphoma(DLBCL)was investigated by bioinformatics analysis.Infection with retroviral vector was performed to establish stable mouse B-cell lymphoma 38B9 cell line with overexpression of Ywhab gene,which was verified by RT-qPCR and Western blot.The impact of Ywhab overexpression on 38B9 cell growth both in vitro and in vivo was detected by cell counting,CCK-8 assay,and subcutaneous tumor loading experiments.The expression of apoptosis-related proteins was detected by RT-qPCR and Western blot.Co-immunoprecipitation combined with mass spectrometry(CoIP-MS)was employed to search for proteins specifically binding to Ywhab gene product 14-3-3β,which was confirmed by Western blot and molecu-lar docking analysis.RESULTS:The Ywhab gene exhibited low expression in DLBCL,which was correlated with poor clinical prognosis of DLBCL patients.Compared with normal mouse bone marrow B cells,Ywhab expression was low in 38B9 cells.Overexpression of Ywhab induced apoptosis of 38B9 cells both in vitro and in vivo,promoted the expression of pro-apoptotic proteins Puma,Noxa and Bax at both mRNA and protein levels,and inhibited the mRNA and protein expres-sion of anti-apoptotic protein Bcl2(P＜0.05).The 14-3-3β protein specifically bound to Hsp90aa1 and reduced Hsp90aa1 protein levels,thereby suppressing the growth of 38B9 cells.CONCLUSION:Ywhab promotes the apoptosis of B-cell lymphoma cells by binding to Hsp90aa1 and thereby inhibiting the function of Hsp90aa1.

AIM:This study aims to investigate the expression differences of the ultraconserved RNA gene cluster uc.102-uc.106 and its host gene Obg-like ATPase 1(OLA1)in various tissues of C57BL/6J mice.METHODS:Five healthy male C57BL/6J mice aged 8～10 weeks were selected,and under normal physiological conditions,various tis-sues and organs,including liver,testis,bone marrow,brain,kidney,blood,lung,colon,thymus,spleen,stomach,heart,lymph nodes and bladder,were collected.Simultaneously,magnetic bead separation technology was employed to extract bone marrow blood cells,including B cells,macrophages,erythroid precursor,and mature red blood cells.The ex-pression levels of uc.102-uc.106 and OLA1 mRNA were determined by RT-qPCR with GAPDH as the internal reference.RESULTS:The OLA1 mRNA exhibited high expression levels in the brain,lymph nodes,testis and thymus of C57BL/6J mice,and low expression levels in the liver,spleen,lung,colon and peripheral blood(P<0.05).The expression trend of the uc.102-uc.106 gene cluster was generally opposite to that of OLA1 mRNA,except for both exhibiting high expression levels in testicular tissue(P<0.05).In blood cells,the OLA1 mRNA and the uc.102-uc.106 gene cluster showed the highest expression levels in B cells and the lowest expression levels in mature red blood cells(P<0.05).CONCLU-SION:This study indicates that OLA1 gene and uc.102-uc.106 gene cluster are expressed in different tissues and blood cells of C57BL/6J mice.Notably,the high expression levels of OLA1 gene and uc.102-uc.106 gene cluster in the mouse testis suggest a potential association with male reproductive functions.