Objective To investigate the influence of recombinant neuregulin-1 beta (rhNRG-1β) on neural stem cell proliferation through extracellular regulated protein kinases (ERK) signaling pathway in oxygen and glucose deprivation (OGD) environment.Methods Neural stem cells were obtained from embryonic brain of mice pregnant for 14-17 d,cultured and identified by immunochemical staining through detection of the indicator nestin using the SABC-FITC (POD)double standard kit.Neural stem cells were divided into three experiment groups (OGD group,control group and OGD + rhNRG-1β group).Control group:identified neural stem cells,2 × 107,were cultured for 3 h in the 24-hole culture plate with DMEM/F12 complete culture medium;OGD group:neural stem cells,2 × 107,were cultured in the 24-hole culture plate deprived glucose DMEM/F12 in a wet airtight container (37 ℃ constant temperature),cells were cultured with mixed gas of nitrogen (950 ml/L) and oxygen (50 ml/L) for 1 h,and then the culture medium was replaced with complete culture medium and cultured for 3 h;OGD + rhNRG-1β group:before OGD intervention,100 μg/L rhNRG-1β was given for 3 h.Neurospheres formation:the three groups of stem cells were dispersed into single cells,1 × 106/ml cells were inoculated to culture plates containing cover slips coated with poly lysine,and cultivated for 7 d,and neurospheres formation of the 3 groups of neural stem cells was observed under microscope,which was aimed to record neural stem cells proliferation changes.Colony formation:the three groups of stem cells,vaccinated in 60 mm in a petri dish,2 × 107 in number,were cultivated in complete culture medium for 24 h.The colony formation of the three groups of cells was observed under microscope,and neural stem cells proliferation changes were observed.Western blotting:the change of phosphorylation ERK (pERK) protein of the three groups of stem cells was determined,and the effect of pERK protein expression regulated by rhNRG-1β in mice neural stem cells proliferation through ERK signaling pathway was observed.Results Microscopically the primary cultured stem cells grew in single or in pairs,in a round shape;neural stem cell proliferated in clumps or colony;neural stem cells expressed the specificity of nestin protein markers with fluorescent yellow-green color.The differences in the aspects of the average diameter of neurospheres and neurospheres quantity in the neural stem cells between groups were statistically significant (F =693.66,1 002.09,all P ＜ 0.01),and among them the neuropheres formation of OGD group was significantly suppressed.Formation quantity and average diameter in OGD group [(88.78 ± 7.14) numbers,(62.12 ± 2.52) μm] were significantly lower than those in the control group [(246.34 ± 8.67) numbers,(128.45 ± 2.33) μm] and those in OGD + rhNRG-1β group [(237.87 ± 6.61) numbers,(118.37 ± 2.71) μm,all P ＜ 0.01].The difference of colony formation rate of neural stem cell was statistically significant (F =132.03,P ＜ 0.01),and among them colony formation of OGD group significantly suppressed.Formation rate in OGD group [(11.65 ± 0.94)％] was significantly lower than that in the control group [(33.23 ± 2.93)％] and that in OGD + rhNRG-1β group [(31.42 ± 2.61)％,all P ＜ 0.01].Western blotting showed that the difference of pERK protein expression of neural stem cells between groups was statistically significant (F =63.76,P ＜ 0.01).Relative expression of the pERK protein in OGD group (0.487 ± 0.072) was significantly lower than that in the control group (1.013 ± 0.112) and that in OGD + rh-NRG-1β group (1.752 ± 0.278,all P ＜ 0.01).Conclusion rhNRG-1β preserves neural stem cell proliferation with phosphorylation ERK protein expression up-regulated in oxygen and glucose deprivation environment.

This paper described the practice of using the bank card as a medical card.This practice is designed to fully leverage the financial service system of major banks in China and premium medical resources,as it may optimize medical service flows of registration,doctors' visit and charging at the hospital.This reform aims at creating an information integrating bank cards and hospital cards featuring practicality,convenience,security and high-tech,so as to improve medical service and provide the patients with a better experience in seeing doctors.

Objective To establish better reaction factors for rep-PCR and to investigate if the extended spectrum ?-lactamase(ESBL)+ Escherichia coli isolated from different patients in the respiratory ward have the same origin by using rep-PCR.Methods The ESBL confirmation was taken by double disc confirmatory test.The susceptibility testing was performed with K-B test.By applying the widespread enterobacter repetitive intergenic consensus as a primer,the stains were typed by rep-PCR following electrophoresis in agarose gel.Results The analysis of the PCR productions indicated that it would create useful DNA ban-prints and all these 22 ESBL(+)Escherichia coli strains were of three origins.Conclusion The method of rep-PCR is practical for epidemiological research in nosocomial infection.

Objective To review the clinical results of percutaneous laser disc decompression(PLDD)in the treatment of cervical disc herniation.Methods PLDD was adopted in the treatment of cer-vi cal disc herniation in32patients.There were19males and13females with an average age of 54years,ranging from33to67years.The lesions were located at the levels of C 3,4 in4cases,C 4,5 in11cases,C 5,6 in13cases and C 6,7 in7cases.The laser fiber was introduced into the herniated disc space with the needle puncturing percu taneously in anterior surface of neck under fluoroscopic guidance.The ND:YAG laser was of 1060nm wave-length,and the diameter of the fiber was400?m.The laser output power was15w with1s emission and5s interval.The compression of spinal cord and surrounding tissue was eliminated by reducing the intra-disc pressure through the vaporization of the disc nucleus.The total laser output power was decid ed depending on the age of the patients,degenerative degree of the disc and the reactive process of heat.The total powers of the group were360J to1100J.Results The mean follow-up was8months,ranging from3to15months.The clinical evaluations were classified as excellent in16cases(50%),good8cases(25%),fair4cases(12.5%)and poor 4cases(12.5%).The general effective ratio was87.5%,the ex cellent and good ratio was75%.The remission ratio of the symptoms was80.7%for the neck and shoulder pain,84.2%for ra-diating pain of the nerve roots of upper extremities,60%for symptoms of the lower ex tremities and78.5%for cervi cal dizziness.No complications occured.Conclusion PLDD could relieve the symptoms and signs of patients cervical disc herniaton.PLDD is easy to manipulate,safe,mini-invasive,and the patients recov-ered sooner with less complications.

Objective To investigate the status quo of formaldehyde pollution in indoor air of the decorated houses. Methods 54 apartments that have been decorated for one month to almost two years and 52 roughcast apartments were selected and the concentrations of formaldehyde were determined by acetylacetone spectrophotometry. Results The results showed that in the same time after decorating, there was significant difference of formaldehyde concentrations between higher and lower temperature periods (P

The Neutral detergent method (AACC method) for determining insoluble dietary fiber in cereals and cereal foods has been used in this experiment. The results showed that the suitable size of the sample was 40-mesh and the better period for digestion in hot neutral detergent solution was one hour for determination. Decahydronaphthalene as a defoaming agent was better than n-octane. The average recoveries of two different ways were from 94.1% to 100.4%. The coefficient of variation for eight wheat samples was 1.8%. Because of its simple equipment, easy operation and good accuracy, this method would be suitable for determination of cereals and cereal foods.

The changes of pathophysiology of perihematomal tissue after intracerebral hemor- rhage are extremely complicated.Studies in recent years have suggested that perihematomal tissue does exist many changes of pathophysiology and molecular biology,such as mass effect of hematoma,hematoma components damage to perihematomal tissue,hemodynamic changes, neuropeptide Y and matrix metalloproteinase changes,etc.

OBJECTIVE: To study the characteristics and regularity of adverse drug reactions (ADR) occurred in our hospital. METHODS: A total of 241 ADR cases occurred in our hospital form Jan. 2006 to Jun. 2009 were analyzed statistically in respect of patients’ status, category of drug, route of administration, organs and system involved in ADR and clinical manifestations. RESULTS: Of the total 241 ADR cases, 69.71%ADR cases were induced by antibacterials. 71.78% ADR cases were induced via intravenous administration. ADR mainly appeared as lesion of skin and appendants (50.92%). CONCLUSION: It is necessary to intervene and guide clinical use of drug, strengthen the monitoring of ADR and reduce the occurrences of ADR.

AIM: To evaluate the diagnostic accuracy of macular ganglion cell - inner plexiform layer ( GCIPL ) measurements using high- definition optical coherence tomography ( Cirrus HD - OCT ) ganglion cell analysis algorithm for detecting early and moderate to severe glaucoma.
METHODS:Twenty normal control persons, 26 patients with early glaucoma and 29 patients with moderate to severe glaucoma were enrolled in this study. Macular GCIPL, optic nerve head ( ONH ) parameters and peripapillary retinal nerve fiber layer ( RNFL ) thickness were measured in each subject. Then all measured results of each parameter were calculated using SPSS17. 0. Areas under the receiver operating characteristic curves ( AUC) of each parameter were calculated to compare the diagnostic accuracy for detecting early and moderate to severe glaucoma.
RESULTS: For detecting early glaucoma, AUC of average RNFL and seven clock value of RNFL were the biggest ( 0. 871 and 0. 896 respectively ), the AUC of parameters in GCIPL were also significant, among them,
the average GCIPL showed bigger AUC(0. 847) than the minimum GCIPL (0. 812). For diagnosing moderate to severe glaucoma, the AUC of rim area was 0. 992, which was bigger than that of average RNFL ( 0. 991 ). The minimum GCIPL showed bigger AUC ( 0. 983 ) than the average GCIPL (0. 967). For early glaucoma diagnosis, the sensitivity of average RNFL was the highest (76. 9%), while the average GCIPL has the highest specificity (93. 5%).
CONCLUSION: AS a new diagnostic parameter for detecting glaucoma, GCIPL shows similar diagnostic potential compared with RNFL. For early glaucoma diagnosis, average RNFL is the most important parameter, while screening early glaucoma, average GCIPL should be paid more attention.

Objective Try to know the prevalence rate of ESBL-s produced by Eseherichia coil in our hospital from 2003 to 2007.Methods Antibiotic susceptibility were determined by K-B disc diffusion.Double-disk synergy tests performed on Mueller-Hinton agar plates was used for ESBL confirmation.Results There was 23.8% of our isolates can produce ESBLs in 2003,25.4% in 2004,31.3% in 2005,36.6% in 2006 and 44.9% in 2007,respectively.The situation of antibiotic resistance is more severe.Conclusion The antibiotic resistance should be well surveyed and the ESBLs must be routinely assayed.