1.Serum-free Aggregate Perfusion Culture of CHO Cells:an Ultrasonic and Sedimentation Column Combined Perfusion System
Zhi LI ; Cheng-Zu XIAO ; Qin YANG ; Xiao-Le HUANG ; Qian-Ru LIANG ; Xiao-Fei CHEN ; Dun-Wu ZHENG ; Xiao-Ming CHEN ;
China Biotechnology 2006;0(04):-
Using the character of natural aggregation of CHO cells, and an ultrasonic and sedimentation column combined perfusion system to promote cells aggregation and retention into bioreactor,recombinant CHO cell strain MK3-A2 was cultured,which could secrete rhTNK-tPA, by a serum-free perfusion culture system. The culture periods in this two experiments were as long as 77 and 110 days respectively. The cells density reached 2?107 cells /ml. The average volumetric productivity of rhTNK-tPA was 89 mg/L?d, and the highest one was 216mg/L?d.The cells aggregation rate was approximately 90%, and the diameters of most of them were 285~570?m. During the perfusion culture the cells retention rate almost kept in 95% and the viability of cells was more than 85%.Thus, it means that aggregation culture with such perfusion system could be used to scale up produce biopharmaceuticals instead of microcarrier culture system.
2.Spatial analysis on the distribution of hemorrhagic fever with renal syndrome by geographic information system in Haidian district, Beijing.
Li-qun FANG ; Wu-chun CAO ; Zhe DUN ; Xiao-ming WU ; Pei-yuan SUN ; Martin KULLDORFF ; Bing-cai WANG ; Hong YANG ; Xiao-wen LI
Chinese Journal of Epidemiology 2003;24(11):1020-1023
OBJECTIVETo analyze the spatial distribution of hemorrhagic fever with renal syndrome (HFRS) in Haidian district, Beijing and to explore the geographical characteristics of HFRS in highly endemic areas.
METHODSAdministration boundary layer was established under the background of 1:100,000 map in the ArcInfo 8.1 software. The HFRS cases from 1997 to 2002 were positioned on the map. Highly endemic areas were identified by spatial cluster analysis using SaTScan 3.0 software. Distribution of HFRS cases was shown in different colors and contours by spatial analysis of geographic information system (GIS).
RESULTSSpatial Cluster Analysis of confirmed cases of HFRS identified in 1997 - 2002 in Haidian district showed that HFRS patients were not randomly distributed. The highly endemic areas were founded in Sujiatuo township, Yongfeng township, Shangzhuang township, Wenquan town and Bei'anhe township (relative risk = 4.43, P = 0.001). A thematic map of HFRS in haidian district was set up.
CONCLUSIONHFRS infections were not randomly distributed, since the distribution was related to geographic-environmental factors.
China ; epidemiology ; Hemorrhagic Fever with Renal Syndrome ; epidemiology ; Humans ; Urban Health ; statistics & numerical data ; Urbanization
3.Molecular Epidemiology and Sequencing of the G-L Intergenic Region of Rabies Viruses Isolated in China
Sheng-Li, MENG ; Ge-Lin, XU ; Jia-Xin, YAN ; Ping-Gang, MING ; Jie, WU ; Xiao-Ming, YANG ; He-Tian, MING ; Feng-Cai, ZHU ; Dun-Jin, ZHOU ; QI-You, XIAO ; Guan-Mu, DONG
Virologica Sinica 2007;22(1):26-33
A group of 25 rabies viruses (RABVs),recovered from 24 dogs and one human case,were collected from various areas in China between 2004 and 2006.Genetic and phylogenetic analyses of the G-L intergenic region were carried out in 25 street RABV isolates and CTN vaccine strains of 7 generations.The study was based on the comparison of a 519 bp nucleotide sequence,encompassing the G-L intergenic region.The nucleotide sequence homologies of Chinese street strains were from 95.5% to 100%.The phylogenetic analysis showed that all Chinese isolates clearly supported the placement of all Chinese viruses in Lyssavirus genotype 1 and they were distributed according to their geographical origins.All of the Chinese strains were closely related but they could still be divided into two groups:group of street strains and group of CTN strains.This study presents details about the molecular epidemiology of rabies viruses based on the sequences of the G-L Intergenic region.
4.Study on the association between hantavirus infection and Rattus norvegicus.
Jia-fu JIANG ; Xiao-ming WU ; Shu-qing ZUO ; Ri-ming WANG ; Li-quan CHEN ; Bing-cai WANG ; Zhe DUN ; Pan-he ZHANG ; Tian-yu GUO ; Wu-chun CAO
Chinese Journal of Epidemiology 2006;27(3):196-199
OBJECTIVETo further understand the association of hantavirus (HV) harbored and transmitted in wild brown rats.
METHODSRattus norvegicus (n = 570) were trapped in 10 sites in Beijing. RT-PCR was used to test rodent lung samples for hantavirus infection. Unconditional multivariate logistic regression analysis was performed, with PCR positive as the dependent variable and the characteristics of Rattus norvegicus population as independent variables.
RESULTSThe overall HV prevalence in Rattus norvegicus was 9.1% (52/570). Significant association between HV infection in Rattus norvegicus and some biological characteristics of host population was observed. Adult Rattus norvegicus had a higher HV prevalence than juveniles. Males in the reproduction periods and rats with wounds were more likely to be infected with HV than others.
CONCLUSIONIt was further confirmed that there existed parallel transmission of HV in Rattus norvegicus hosts. Aggression might be the primary mode of HV transmission among male Rattus norvegicus.
Aggression ; Animals ; Animals, Wild ; injuries ; virology ; China ; epidemiology ; Female ; Hantavirus ; isolation & purification ; Hantavirus Infections ; epidemiology ; transmission ; veterinary ; virology ; Logistic Models ; Lung ; virology ; Male ; Prevalence ; Rats ; injuries ; virology ; Reproduction ; Reverse Transcriptase Polymerase Chain Reaction ; Risk Factors ; Rodent Diseases ; epidemiology ; transmission
5.Study on the molecular epidemiology of hantaviruse carried by hosts in northern suburb of Beijing.
Shu-qing ZUO ; Xiao-ming WU ; Pei-yuan SUN ; Pan-he ZHANG ; Bing-cai WANG ; Fang TANG ; Zhe DUN ; Wei CAI ; Qi LIU ; Fan-xin ZENG ; Jia-fu JIANG ; Wei LIU ; Wu-chun CAO
Chinese Journal of Epidemiology 2004;25(5):421-424
OBJECTIVETo investigate hantanvirus infection of captured rodents in Haidian district and Changping district of Beijing and to type hantavirus using molecular technique.
METHODSThe captured mice were classified and the density of distribution was calculated. Reverse transcription-polymerase chain reaction (RT-PCR) technique was used to amplify the partial M fragnments of hantaviruse. Several representative positive samples were sequenced and analysed by ClustalX (5.0) and DNAClub software.
RESULTSA total of 414 animals were captured, among which Battus norvegicus was the dominant group. In Haidian district, the median infection rates with hantavirus were 13.14% in Battus norvegicus and 0 in Mus musculus Linnaeus. In Changping district, the average infection rates were 17.46% in Battus norvegicus and 3.57% in Mus musculus Linnaeus. Nucleotide sequences analysis showed that the virus detected all belonged to SEO-type. They clustered with Z37 virus and could be branched into 2 different subclades.
CONCLUSIONThe major hosts of hantavirus in Haidian and Changping district were Battus norvegicus and the epidemic strains in the two districts of Beijing were genotyped as SEO-type. Nucleotide sequence and deduced amino acid sequence from different rodents were highly homologous, while nucleotide mutation had also been observed. Further studies are required to explore the possible virus sequence mutation.
Animals ; China ; epidemiology ; DNA, Viral ; genetics ; Disease Reservoirs ; Fluorescent Antibody Technique ; Hantavirus ; classification ; genetics ; isolation & purification ; Hantavirus Infections ; epidemiology ; veterinary ; virology ; Hemorrhagic Fever with Renal Syndrome ; epidemiology ; veterinary ; virology ; Mice ; Molecular Epidemiology ; Phylogeny ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; Rodent Diseases ; epidemiology ; virology
6.Clinical efficacy and safety of controlled-release dinoprostone insert: a multicenter retrospective study.
Xue-Yuan LI ; Yue-Wen GUO ; Yan-Wen XU ; Bin ZHU ; Xue-Xue WU ; Xiang CHEN ; Xiao-Yi WANG ; Dun-Jin CHEN ; Hui CHEN ; Jian-Ping ZHANG ; Zhi-Jian WANG ; Sheng-Li AN
Journal of Southern Medical University 2017;37(1):18-23
OBJECTIVETo analyze the effectiveness and safety of controlled-release dinoprostone insert for term labor induction in the Pearl River Delta of Guangdong province.
METHODSTwenty hospitals using controlled-release dinoprostone insert for term labor induction in the Pearl River Delta of Guangdong province were stratified into provincial hospitals and municipal hospitals, and three hospitals of each level were selected as research units. According to the inclusion and exclusion criteria, 1390 pregnant women receiving term labor induction using controlled-release dinoprostone insert were retrospectively analyzed to evaluate the the effectiveness and safety with another 957 pregnant women with induced abortion using oxytocin as the control group.
RESULTSCompared with the control group, the controlled-release dinoprostone insert group showed a significantly longer length of the latent phase of labor (4.06∓2.65 vs 3.20∓2.08 h, P=0.003, 95%CI [0.182, 0.920]) and shorter lengths of the active phase (1.73∓1.32 vs 2.22∓1.75 h, P=0.000, 95%CI [-0.795, -0.363]) and the second stage of labor (0.49∓0.37 vs 0.54∓0.43 h, P=0.003, 95%CI [-0.137, -0.028]). No significant differences were found in the length of the first stage of labor, the vaginal delivery rate, adverse reactions, or fetal outcomes between the two groups.
CONCLUSIONControlled-release dinoprostone insert is effective and safe for labor induction at term.
7.Distribution and interaction of bone marrow mesenchymal stem cells and cord blood CIK/NK cells infused via different ways at different time periods in NOD/SCID mice.
Yang LI ; Xiao-Ping WANG ; Hai-Xia GUO ; Ke HUANG ; Jing WEI ; Dun-Hua ZHOU ; Wen-Ge HUANG ; Yan-Feng WU ; Shao-Liang HUANG
Journal of Experimental Hematology 2010;18(1):140-145
The study was aimed to explore the distribution and interaction mechanism of human bone marrow mesenchymal stem cells (MSC) and cord blood cytokine-induced killer (CIK)/natural killer (NK) cells infused via different ways at different times in NOD/SCID mice. 5 microl 1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethylindocarbocyanine perchlorate (DiI) dye(red) was added in suspension of MSC per ml, and 1 microl carboxyfluorescein diacetate, succinimidyl ester(CFDA SE) dye(green) was added in suspension of CIK/NK cells per ml. The amounts of MSC and CIK/NK cells infused in each 6 NOD/SCID mouse were 1 x 10(6) (0.1 ml) and 1 x 10(7) (0.1 ml) respectively. All mice were divided into 4 groups, each group consisted of 6 mice. Group A: MSC (intravenous infusion, iv) + CIK/NK cells (iv) at the same time, group B: MSC (iv) + CIK/NK cells (iv) at 48 hours after infusion of MSC; group C: MSC (intramedullary infusion, im) + CIK/NK cells (iv) at the same time; group D: MSC (im) + CIK/NK cells (iv) at 48 hours after infusion of MSC. 3 NOD/SCID mice were sacrificed per batch at 24 hours and 48 hours after infused CIK/NK cells. Frozen sections of liver, spleen, lung and kidney were prepared, and then followed by counting the amounts of red and green fluorescence cells under fluorescence microscope, and calculating the ratio of MSC to CIK/NK cells for reflecting the interaction of MSC and CIK/NK cells in mice, and for showing the suppressive intensity of MSCs on CIK/NK cells. The results showed that the sums of average ratios of MSC to CIK/NK cells in lung, liver and spleen of group A and B were higher than that in group C and D at 24 hours and 48 hours respectively after infusing CIK/NK cells. The sum of average ratios of MSC to CIK/NK cells in group A was slightly higher than that in group B at 24 hours and 48 hours after infusing CIK/NK cells, but there was no significant difference between them. The sum of average ratios of MSC to CIK/NK cells in lung, liver and spleen in group C was slightly lower than that in group D at 24 hours after infusing CIK/NK cells, but reversed at 48 hours later and there was no significant difference between them. The sums of average ratios of MSC to CIK/NK cells in lung, liver and spleen in group A, B, C and D were all higher than those in kidney at 24 and 48 hours respectively after infusing CIK/NK cells. It is concluded, the MSC and CIK/NK cells may interact if they are infused via the same way and at the same time, the location where the suppression of MSC on CIK/NK cells occur in vivo may be reticulo-endothelial systems in lungs and livers.
Animals
;
Bone Marrow Transplantation
;
Cell Communication
;
Cytokine-Induced Killer Cells
;
transplantation
;
Fetal Blood
;
cytology
;
Humans
;
Killer Cells, Natural
;
transplantation
;
Liver
;
cytology
;
Lung
;
cytology
;
Mesenchymal Stem Cell Transplantation
;
Mice
;
Mice, Inbred NOD
;
Mice, SCID
;
Transplantation, Heterologous
8.Competency Model of Physical Therapists in China: An Exploratory Research
Dun-wu XIAO ; Nan CHEN ; Yong WANG ; Zhuo-ying QIU ; Li-qi YI ; Ping ZHU
Chinese Journal of Rehabilitation Theory and Practice 2021;27(3):341-348
Objective:To construct the competency model of physical therapists in China. Methods:A set of physical therapists' competency elements was established based on literature review and expert interviews. A questionnaire was designed based on the set, and 641 rehabilitation practitioners were investigated (521 valid questionnaires returned) with the questionnaire. Exploration factor analysis was used to construct the competency model of physical therapists, and confirmatory factor analysis was used to confirmed. Results:The set of physical therapists' competency elements included 74 elements in four dimensions. There were 44 competency elements in the competency model of physical therapists, including five dimensions that were Professionalism and Moral Conduct, Knowledge and Skills of Evaluation and Intervention, Abilities of Educational-Research and Advocate, Abilities of Cooperation and Management, and Basic Knowledge of Discipline, covering 74.41% of the total variance. The Cronbach's α coefficient of the whole questionnaire and each dimension was more than 0.8. Model fitness indexes of confirmatory factor analysis were as follow:
9.Effects of Aquatic Therapeutic Exercise on Motor Function and Activities for Children and Youth with Cerebral Palsy: A Systematic Review and Meta-Analysis
Yao CUI ; Dun-wu XIAO ; Lin DING ; Fu-bing QIU ; Fang CONG ; Zhuo-ying QIU
Chinese Journal of Rehabilitation Theory and Practice 2021;27(1):79-92
Objective:To evaluate the intervention effects of aquatic therapeutic exercise on functioning and quality of life for children and youth with cerebral palsy within framework of World Health Organization Family International Classifications using systematic review. Methods:Literatures were retrieved and reviewed from the databases of Cochrane Library, PEDro, PubMed, EMBASE, Web of Knowledge, Web of Science, OVID, EBSCO, CMCI, CNKI, Wangfang and VIP until May, 2020. The randomized controlled trials (RCTs) about aquatic therapeutic exercise for children and youth with cerebral palsy for functioning were systematically reviewed. The data were analyzed with RevMan 5.3. Results:Nine RCTs were selected, including 162 children and youth. The score of Gross Motor Function Measure improved significantly after aquatic exercise (WMD = 6.31, 95 %CI 2.57 to 10.06,
10.Effects of Aquatic Exercise on Lower-limb Motor Function and Activities of Daily Living for Patients with Stroke: A Meta-analysis
Yao CUI ; Wei JIA ; Ming ZENG ; Fang CONG ; Long JIN ; Feng-shan SI ; Bin YAO ; Dun-wu XIAO ; Kai ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2020;26(3):263-277
Objective:To evaluate the effects of aquatic exercise on lower-limb motor function and activities of daily living for patients with stroke. Methods:The randomized controlled trials (RCTs) about effects of aquatic therapeutic exercise on stroke patients were recalled from the databases of Cochrane Library, PEDro, PubMed, EMBASE, Web of Knowledge, Web of Science, OVID, EBSCO, CMCI, CNKI, Wanfang and VIP. The methodological quality of the included RCTs was evaluated. The data were extracted, and analysed with RevMan 5.3. Results:A total of 23 RCTs that represented 861 participants were evaluated. Compared with the control group, aquatic exercise significantly improved the performance of Berg Balance Scale (WMD = 2.69, 95%CI 1.21 to 4.16,